Home LiteratureArticle Details
PMID: 7896767 Published · ppublish English Journal Article Review

Cation transport systems in mitochondria: Na+ and K+ uniports and exchangers.

Journal of bioenergetics and biomembranes ·Vol. 26 ·No. 5 ·1994-10-00 ·Pages 519-26

Brierley GP, Baysal K, Jung DW

Abstract

It is now well established that mitochondria contain three antiporters that transport monovalent cations. A latent, allosterically regulated K+/H+ antiport appears to serve as a cation-extruding device that helps maintain mitochondrial volume homeostasis. An apparently unregulated Na+/H+ antiport keeps matrix [Na+] low and the Na(+)-gradient equal to the H(+)-gradient. A Na+/Ca2+ antiport provides a Ca(2+)-extruding mechanism that permits the mitochondrion to regulate matrix [Ca2+] by balancing Ca2+ efflux against influx on the Ca(2+)-uniport. All three antiports have well-defined physiological roles and their molecular properties and regulatory features are now being determined. Mitochondria also contain monovalent cation uniports, such as the recently described ATP- and glibenclamide-sensitive K+ channel and ruthenium red-sensitive uniports for Na+ and K+. A physiological role of such uniports has not been established and their properties are just beginning to be defined.

MeSH Terms
Animals Carrier Proteins/metabolism Cations, Monovalent/metabolism Mitochondria/metabolism Potassium/metabolism Sodium/metabolism Sodium-Calcium Exchanger Sodium-Hydrogen Exchangers/metabolism
Chemicals
Carrier Proteins Cations, Monovalent Sodium-Calcium Exchanger Sodium-Hydrogen Exchangers Sodium Potassium
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Brierley G P
Department of Medical Biochemistry, Ohio State University, Columbus 43210.
Baysal K
Jung D W
References (63)
63 references, click to expand
  1. Purification of a reconstitutively active K+/H+ antiporter from rat liver mitochondria.
    J Biol Chem. 1990 Sep 5;265(25):15316-22 PMID: 2394723
  2. Evidence for the existence of regulatory sites for Ca2+ on the Na+/Ca2+ carrier of cardiac mitochondria.
    Biochem J. 1982 Feb 15;202(2):509-18 PMID: 7092829
  3. A mitochondrial protein fraction catalyzing transport of the K+ analog T1+.
    FEBS Lett. 1990 Oct 29;273(1-2):215-8 PMID: 1699808
  4. Reconstitution of the beef heart and rat liver mitochondrial K+/H+ (Na+/H+) antiporter. Quantitation of K+ transport with the novel fluorescent probe, PBFI.
    J Biol Chem. 1990 Jun 25;265(18):10522-6 PMID: 2162352
  5. Purification and patch clamp analysis of a 40-pS channel from rat liver mitochondria.
    Biochemistry. 1992 Mar 3;31(8):2223-9 PMID: 1371695
  6. Translocation of some anions cations and acids in rat liver mitochondria.
    Eur J Biochem. 1969 Jun;9(2):149-55 PMID: 5804494
  7. On the regulation of K+ uniport in intact mitochondria by adenine nucleotides and nucleotide analogs.
    J Biol Chem. 1993 Jan 15;268(2):997-1004 PMID: 8380420
  8. Ba2+ ions inhibit the release of Ca2+ ions from rat liver mitochondria.
    Biochim Biophys Acta. 1985 Sep 19;809(2):160-6 PMID: 2412581
  9. Changes in permeability to protons and other cations at high proton motive force in rat liver mitochondria.
    Biochem J. 1986 Feb 15;234(1):75-81 PMID: 3010957
  10. The cardiac Na(+)-Ca2+ exchanger: relative rates of calcium and sodium movements and their modulation by protonation-deprotonation of the carrier.
    Biochemistry. 1994 Jan 11;33(1):312-9 PMID: 8286352
  11. Reconstitution, identification, purification, and immunological characterization of the 110-kDa Na+/Ca2+ antiporter from beef heart mitochondria.
    J Biol Chem. 1992 Sep 5;267(25):17983-9 PMID: 1517231
  12. A gated pathway for electrophoretic Na+ fluxes in rat liver mitochondria. Regulation by surface Mg2+.
    Eur J Biochem. 1990 Feb 22;188(1):91-7 PMID: 2156695
  13. Matrix magnesium and the permeability of heart mitochondria to potassium ion.
    J Biol Chem. 1986 May 15;261(14):6408-15 PMID: 3084482
  14. Evidence for the allosteric regulation of the mitochondrial K+/H+ antiporter by matrix protons.
    J Biol Chem. 1990 Feb 15;265(5):2538-45 PMID: 2154450
  15. Mitochondrial transport of K+ and Mg2+.
    Biochim Biophys Acta. 1987;895(3):155-65 PMID: 3333013
  16. Respiration-dependent contraction of swollen heart mitochondria: participation of the K+/H+ antiporter.
    J Bioenerg Biomembr. 1988 Apr;20(2):229-42 PMID: 3372495
  17. Na+/H+ exchange in mitochondria as monitored by BCECF fluorescence.
    FEBS Lett. 1989 Jul 17;251(1-2):49-52 PMID: 2546827
  18. Regulation of mitochondrial K+/H+ antiport activity by hydrogen ions.
    Arch Biochem Biophys. 1991 Aug 1;288(2):358-67 PMID: 1898035
  19. Regulation of the mitochondrial matrix volume in vivo and in vitro. The role of calcium.
    Biochem J. 1986 Jun 15;236(3):779-87 PMID: 2431681
  20. A role for the mitochondrial Na(+)-Ca2+ exchanger in the regulation of oxidative phosphorylation in isolated heart mitochondria.
    J Biol Chem. 1993 Jan 15;268(2):938-47 PMID: 8419373
  21. Na(+)-dependent Ca2+ efflux mechanism of heart mitochondria is not a passive Ca2+/2Na+ exchanger.
    Am J Physiol. 1994 Mar;266(3 Pt 1):C800-8 PMID: 8166244
  22. Reconstitution and partial purification of the glibenclamide-sensitive, ATP-dependent K+ channel from rat liver and beef heart mitochondria.
    J Biol Chem. 1992 Dec 25;267(36):26062-9 PMID: 1464617
  23. Transmembrane gradients of free Na+ in isolated heart mitochondria estimated using a fluorescent probe.
    Am J Physiol. 1992 Apr;262(4 Pt 1):C1047-55 PMID: 1566810
  24. Kinetics of inhibition and binding of dicyclohexylcarbodiimide to the 82,000-dalton mitochondrial K+/H+ antiporter.
    J Biol Chem. 1986 Sep 15;261(26):12300-5 PMID: 3745188
  25. Reconstitution of the mitochondrial non-selective Na+/H+ (K+/H+) antiporter into proteoliposomes.
    J Biol Chem. 1989 Apr 5;264(10):5846-51 PMID: 2538430
  26. The calcium-induced and sodium-induced effluxes of calcium from heart mitochondria. Evidence for a sodium-calcium carrier.
    Eur J Biochem. 1977 Oct 3;79(2):549-58 PMID: 923566
  27. Quinine inhibition of Na+ and K+ transport provides evidence for two cation/H+ exchangers in rat liver mitochondria.
    J Biol Chem. 1982 Aug 25;257(16):9252-4 PMID: 6286609
  28. The uptake and extrusion of monovalent cations by isolated heart mitochondria.
    Mol Cell Biochem. 1976 Jan 31;10(1):41-63 PMID: 2858
  29. The permeability of uncoupled heart mitochondria to potassium ion.
    J Biol Chem. 1984 Jun 10;259(11):6904-11 PMID: 6202687
  30. Functional properties of the reconstituted phosphate carrier from bovine heart mitochondria: evidence for asymmetric orientation and characterization of three different transport modes.
    Biochim Biophys Acta. 1993 Jun 18;1149(1):40-8 PMID: 8318530
  31. Activation of latent K+ uniport in mitochondria treated with the ionophore A23187.
    J Biol Chem. 1989 Nov 15;264(32):18902-6 PMID: 2509471
  32. Characterization of the mitochondrial Na+-H+ exchange. The effect of amiloride analogues.
    Biochim Biophys Acta. 1988 Oct 20;944(3):383-90 PMID: 2846061
  33. Sodium/proton antiporter of rat liver mitochondria.
    FEBS Lett. 1980 Aug 11;117(1):39-43 PMID: 6250900
  34. Kinetic properties of the K+/H+ antiport of heart mitochondria.
    Biochemistry. 1990 Jan 16;29(2):408-15 PMID: 2105743
  35. Measurement of matrix free Mg2+ concentration in rat heart mitochondria by using entrapped fluorescent probes.
    Biochem J. 1990 Nov 1;271(3):627-34 PMID: 2244870
  36. The regulation of the matrix volume of mammalian mitochondria in vivo and in vitro and its role in the control of mitochondrial metabolism.
    Biochim Biophys Acta. 1989 Mar 23;973(3):355-82 PMID: 2647140
  37. Energy-dependent contraction of swollen heart mitochondria--activation by butacaine.
    Arch Biochem Biophys. 1979 Mar;193(1):76-87 PMID: 36855
  38. Swelling and contraction of potato mitochondria.
    Plant Physiol. 1979 Dec;64(6):948-53 PMID: 16661111
  39. K+/H+ antiport in heart mitochondria.
    J Biol Chem. 1984 Dec 10;259(23):14672-8 PMID: 6438102
  40. The Ba2+ sensitivity of the Na+-induced Ca2+ efflux in heart mitochondria: the site of inhibitory action.
    Biochim Biophys Acta. 1986 Jun 13;858(1):125-34 PMID: 3707957
  41. Regulation of the mitochondrial Na+/Ca2+ antiport by matrix pH.
    Arch Biochem Biophys. 1991 Dec;291(2):383-9 PMID: 1952951
  42. ATP-sensitive K+ channel in the mitochondrial inner membrane.
    Nature. 1991 Jul 18;352(6332):244-7 PMID: 1857420
  43. Reconstitution and partial purification of the Na(+)-selective Na+/H+ antiporter of beef heart mitochondria.
    J Biol Chem. 1991 Apr 5;266(10):6518-23 PMID: 1848857
  44. Reconstitution of transmembrane K+ transport with a 53 kilodalton mitochondrial protein.
    Biochem Biophys Res Commun. 1988 May 31;153(1):224-30 PMID: 3377787
  45. The stoichiometry of the exchange catalysed by the mitochondrial calcium/sodium antiporter.
    Biochem J. 1985 Jul 1;229(1):161-6 PMID: 3929768
  46. The cycling of calcium, sodium, and protons across the inner membrane of cardiac mitochondria.
    Eur J Biochem. 1978 Nov 15;91(2):599-608 PMID: 32035
  47. On the mechanism by which dicyclohexylcarbodiimide and quinine inhibit K+ transport in rat liver mitochondria.
    J Biol Chem. 1986 Feb 5;261(4):1529-35 PMID: 3944099
  48. Evidence for the existence of an inner membrane anion channel in mitochondria.
    Biochim Biophys Acta. 1986;853(3-4):187-204 PMID: 2441746
  49. The K+ conductance of the inner mitochondrial membrane. A study of the inducible uniport for monovalent cations.
    J Biol Chem. 1991 May 25;266(15):9465-70 PMID: 1903386
  50. Evidence for three different electrophoretic pathways in yeast mitochondria: ion specificity and inhibitor sensitivity.
    J Bioenerg Biomembr. 1993 Dec;25(6):671-8 PMID: 8144494
  51. N,N'-Dicyclohexylcarbodiimide inhibits monovalent cation influx but not cation/proton exchange in heart mitochondria.
    J Biol Chem. 1980 Jan 25;255(2):408-12 PMID: 7356623
  52. Energy-dependent contraction of swollen heart mitochondria.
    J Biol Chem. 1977 Nov 25;252(22):7932-9 PMID: 21185
  53. The Na+/H+ exchanger: an update on structure, regulation and cardiac physiology.
    Biochem J. 1993 Dec 1;296 ( Pt 2):273-85 PMID: 8257412
  54. Electroneutral H+-K+ exchange in liver mitochondria. Regulation by membrane potential.
    Biochim Biophys Acta. 1983 Aug 31;724(2):212-23 PMID: 6309221
  55. K+/H+ antiport in mitochondria.
    J Bioenerg Biomembr. 1988 Apr;20(2):193-209 PMID: 3286632
  56. Mechanisms by which mitochondria transport calcium.
    Am J Physiol. 1990 May;258(5 Pt 1):C755-86 PMID: 2185657
  57. The plasma membrane sodium-hydrogen exchanger and its role in physiological and pathophysiological processes.
    Circ Res. 1985 Jun;56(6):773-88 PMID: 2988813
  58. On the nature of ion leaks in energy-transducing membranes.
    Biochim Biophys Acta. 1989 Sep 28;976(2-3):109-20 PMID: 2675980
  59. On the regulation of Na+/H+ and K+/H+ antiport in yeast mitochondria: evidence for the absence of an Na(+)-selective Na+/H+ antiporter.
    Biochim Biophys Acta. 1993 Oct 4;1144(3):367-73 PMID: 8399283
  60. On the mechanism of regulation of the mitochondrial K+/H+ exchanger.
    J Biol Chem. 1980 Dec 10;255(23):11273-9 PMID: 7440541
  61. Ruthenium red inhibits mitochondrial Na+ and K+ uniports induced by magnesium removal.
    J Biol Chem. 1990 Oct 25;265(30):18063-6 PMID: 1698784
  62. The Na(+)-independent Ca2+ efflux mechanism of liver mitochondria is not a passive Ca2+/2H+ exchanger.
    J Biol Chem. 1991 Nov 15;266(32):21640-8 PMID: 1939193
  63. Kinetic properties of the Na+/H+ antiport of heart mitochondria.
    Biochemistry. 1989 May 16;28(10):4347-54 PMID: 2548576
Article Info
Journal
Journal of bioenergetics and biomembranes
Abbr.
J Bioenerg Biomembr
ISSN
0145-479X
DOI
10.1007/BF00762736
Published
1994-10-00
Pages
519-26
Language
English
Region
United States
NLM ID
7701859
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00762736 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2