The stoichiometry of the exchange catalysed by the mitochondrial calcium/sodium antiporter.

The Biochemical journal ·Vol. 229 ·No. 1 ·1985-07-01 ·Pages 161-6

Brand MD

Abstract

Rat heart mitochondria respiring on succinate in the presence of Ruthenium Red (to inhibit uptake on the Ca2+ uniporter) released Ca2+ on the calcium/sodium antiporter until a steady state was reached. Addition of the ionophore A23187 (which catalyses Ca2+/2H+ exchange) did not perturb this steady state. Thermodynamic analysis showed that if a Ca2+/nNa+ exchange with any value of n other than 2 was at equilibrium, addition of A23187 would cause an obvious change in extramitochondrial free [Ca2+]. Therefore the endogenous calcium/sodium antiporter of mitochondria catalyses electroneutral Ca2+/2Na+ exchange.

MeSH Terms

Animals Calcimycin/pharmacology Calcium/metabolism Carrier Proteins/metabolism Hydrogen-Ion Concentration In Vitro Techniques Membrane Proteins/metabolism Methylamines/metabolism Mitochondria, Heart/drug effects,metabolism Onium Compounds/metabolism Rats Sodium/metabolism Sodium-Calcium Exchanger Thermodynamics Trityl Compounds/metabolism

Chemicals

Carrier Proteins Membrane Proteins Methylamines Onium Compounds Sodium-Calcium Exchanger Trityl Compounds triphenylmethylphosphonium Calcimycin Sodium methylamine Calcium

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Brand M D

References (26)

26 references, click to expand

Ruthenium red and violet. I. Chemistry, purification, methods of use for electron microscopy and mechanism of action.
Anat Rec. 1971 Nov;171(3):347-68 PMID: 4108333
A23187: a divalent cation ionophore.
J Biol Chem. 1972 Nov 10;247(21):6970-7 PMID: 4263618
Some effects of ionophore A23187 on energy utilization and the distribution of cations and anions in mitochondria.
Biochemistry. 1976 Jun 15;15(12):2690-7 PMID: 820370
The calcium-induced and sodium-induced effluxes of calcium from heart mitochondria. Evidence for a sodium-calcium carrier.
Eur J Biochem. 1977 Oct 3;79(2):549-58 PMID: 923566
The interrelations between the transport of sodium and calcium in mitochondria of various mammalian tissues.
Eur J Biochem. 1978 Jan 2;82(1):25-31 PMID: 23291
Calcium transport and porton electrochemical potential gradient in mitochondria from guinea-pig cerebral cortex and rat heart.
Biochem J. 1978 Mar 15;170(3):511-22 PMID: 348200
The cycling of calcium, sodium, and protons across the inner membrane of cardiac mitochondria.
Eur J Biochem. 1978 Nov 15;91(2):599-608 PMID: 32035
The regulation of extramitochondrial free calcium ion concentration by rat liver mitochondria.
Biochem J. 1978 Nov 15;176(2):463-74 PMID: 33670
The resolution of calcium fluxes in heart and liver mitochondria using the lanthanide series.
FEBS Lett. 1979 Aug 15;104(2):352-4 PMID: 477998
Mitochondrial calcium transport.
FEBS Lett. 1980 Mar 10;111(2):261-8 PMID: 6987089
Na+ releases Ca2+ from liver, kidney and lung mitochondria.
FEBS Lett. 1980 Feb 11;110(2):216-8 PMID: 7371826
Effects of monovalent cations on Ca2 transport in mitochondria; a comparison between brown fat and liver mitochondria from rat.
Acta Chem Scand B. 1980;34(2):149-51 PMID: 7415742
The Ca2+-Na+ antiporter of heart mitochondria operates electroneutrally.
Biochem Biophys Res Commun. 1980 Jul 16;95(1):193-6 PMID: 7417249
On the role of the calcium transport cycle in heart and other mammalian mitochondria.
FEBS Lett. 1980 Sep 22;119(1):1-8 PMID: 7000543
Stimulation of calcium-ion efflux from liver mitochondria by sodium ions and its response to ADP and energy state.
Biochem J. 1981 Mar 15;194(3):925-9 PMID: 6171263
Selective inhibition of Na+-induced Ca2+ release from heart mitochondria by diltiazem and certain other Ca2+ antagonist drugs.
J Biol Chem. 1982 Jun 10;257(11):6000-2 PMID: 6176579
Evidence for the existence of regulatory sites for Ca2+ on the Na+/Ca2+ carrier of cardiac mitochondria.
Biochem J. 1982 Feb 15;202(2):509-18 PMID: 7092829
Determination of the matrix free Ca2+ concentration and kinetics of Ca2+ efflux in liver and heart mitochondria.
J Biol Chem. 1982 Aug 10;257(15):8696-704 PMID: 6807979
Evidence for beta-adrenergic activation of Na+-dependent efflux of Ca2+ from isolated liver mitochondria.
Biochem J. 1982 Apr 15;204(1):369-71 PMID: 7115328
Mitochondrial calcium transport.
Biochim Biophys Acta. 1982 Sep 1;683(1):57-88 PMID: 6291604
The stimulation of the release of Ca2+ from mitochondria by sodium ions and its inhibition.
Arch Biochem Biophys. 1982 Oct 15;218(2):531-9 PMID: 7159098
The activation of Na+-dependent efflux of Ca2+ from liver mitochondria by glucagon and beta-adrenergic agonists.
Biochem J. 1983 Feb 15;210(2):463-72 PMID: 6134523
The stoichiometry of the cardiac sodium-calcium exchange system.
J Biol Chem. 1984 Jun 25;259(12):7733-9 PMID: 6736024
Na+-dependent regulation of extramitochondrial Ca2+ by rat-liver mitochondria.
Eur J Biochem. 1984 Oct 1;144(1):159-68 PMID: 6479169
Thermodynamic control of electron flux through mitochondrial cytochrome bc1 complex.
Biochem J. 1985 Jan 15;225(2):399-405 PMID: 2983670
Electroneutral efflux of Ca2+ from liver mitochondria.
Biochem J. 1985 Jan 15;225(2):413-9 PMID: 2983672

Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2290161

Published

1985-07-01

Pages

161-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1145162

Subset

External Links

PubMed source DOI: 10.1042/bj2290161 PMC Full Text View journal details

Analysis Services

The stoichiometry of the exchange catalysed by the mitochondrial calcium/sodium antiporter.

Contact

WeChat Official Account