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PMID: 7894010 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Cytoplasmic HSP70 homologues of pea: differential expression in vegetative and embryonic organs.

Plant molecular biology ·Vol. 27 ·No. 3 ·1995-02-00 ·Pages 441-56

DeRocher A, Vierling E

Abstract

Eukaryotes express several cytoplasmic HSP70 genes, and their encoded proteins participate in diverse cellular processes. Three cDNAs encoding highly expressed cytoplasmic HSP70 homologues from Pisum sativum were cloned and characterized. They were designated PsHSP71.2, PsHSC71.0, and PsHSP70b. These HSP70 genes have different expression profiles in leaves: PsHSP71.2 is observed only in response to heat stress, PsHSC71.0 is present constitutively, and PsHSP70b is weakly constitutively expressed, but induced strongly in response to heat stress. In addition to being heat induced, the PsHSP71.2 mRNA is also expressed in zygotic, but not maternal organs of developing pea seeds, while PsHSC71.0 and PsHSP70b mRNAs are present in maternal and zygotic organs throughout seed development. Immunoblot analysis of parallel protein samples detects a 70 kDa polypeptide in all samples, and a 72 kDa polypeptide that corresponds to the PsHSP71.2 gene product is observed in cotyledons beginning at mid-maturation and in axes beginning between late maturation and desiccation. This polypeptide is not detected in the seed coat. The 72 kDa polypeptide remains abundant in both cotyledons and axes through germination, but declines substantially between 48 and 72 h after the onset of inbibition. Differential control of HSP70 expression during heat stress, seed maturation, and germination is consistent with the hypothesis that there are functional distinctions between cytoplasmic HSP70s.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Cytoplasm/metabolism DNA, Complementary/genetics DNA, Plant/genetics Gene Expression Regulation, Plant Genes, Plant/genetics Germination HSP70 Heat-Shock Proteins/biosynthesis,genetics Molecular Sequence Data Peas/genetics,growth & development,metabolism RNA, Messenger/biosynthesis RNA, Plant/biosynthesis Seeds/metabolism Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
DNA, Complementary DNA, Plant HSP70 Heat-Shock Proteins RNA, Messenger RNA, Plant
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
DeRocher A
Department of Molecular and Cellular Biology, University of Arizona, Tucson 85721.
Vierling E
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00019312
Published
1995-02-00
Pages
441-56
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
Databases
GENBANK
U08820, U08848, Z32537
External Links
PubMed source DOI: 10.1007/BF00019312 View journal details
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