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PMID: 7865877 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

TGN38 recycles basolaterally in polarized Madin-Darby canine kidney cells.

Molecular biology of the cell ·Vol. 5 ·No. 10 ·1994-10-00 ·Pages 1093-103

Rajasekaran AK, Humphrey JS, Wagner M, Miesenböck G, Le Bivic A, Bonifacino JS, Rodriguez-Boulan E

Abstract

Sorting of newly synthesized plasma membrane proteins to the apical or basolateral surface domains of polarized cells is currently thought to take place within the trans-Golgi network (TGN). To explore the relationship between protein localization to the TGN and sorting to the plasma membrane in polarized epithelial cells, we have expressed constructs encoding the TGN marker, TGN38, in Madin-Darby canine kidney (MDCK) cells. We report that TGN38 is predominantly localized to the TGN of these cells and recycles via the basolateral membrane. Analyses of the distribution of Tac-TGN38 chimeric proteins in MDCK cells suggest that the cytoplasmic domain of TGN38 has information leading to both TGN localization and cycling through the basolateral surface. Mutations of the cytoplasmic domain that disrupt TGN localization also lead to nonpolarized delivery of the chimeric proteins to both surface domains. These results demonstrate an apparent equivalence of basolateral and TGN localization determinants and support an evolutionary relationship between TGN and plasma membrane sorting processes.

Related Genes
TGN38
MeSH Terms
Amino Acid Sequence Animals Biomarkers Cell Line Cell Membrane/metabolism Cell Polarity Dogs Fluorescent Antibody Technique Glycoproteins Golgi Apparatus/metabolism Kidney/cytology,metabolism Membrane Glycoproteins/genetics,metabolism Membrane Proteins Molecular Sequence Data Recombinant Fusion Proteins/genetics,metabolism
Chemicals
Biomarkers Glycoproteins Membrane Glycoproteins Membrane Proteins Recombinant Fusion Proteins
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Rajasekaran A K
Department of Cell Biology and Anatomy, Cornell University Medical College, New York, New York 10021.
Humphrey J S
Wagner M
Miesenböck G
Le Bivic A
Bonifacino J S
Rodriguez-Boulan E
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Article Info
Journal
Molecular biology of the cell
Abbr.
Mol Biol Cell
ISSN
1059-1524
DOI
10.1091/mbc.5.10.1093
Published
1994-10-00
Pages
1093-103
Language
English
Region
United States
NLM ID
9201390
PMCID
PMC301133
Subset
IM
Grants
NIGMS NIH HHS · R01 GM034107 · United States
NIGMS NIH HHS · GM-34107 · United States
External Links
PubMed source DOI: 10.1091/mbc.5.10.1093 PMC Full Text View journal details
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