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PMID: 1860878 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A single amino acid change in the cytoplasmic domain alters the polarized delivery of influenza virus hemagglutinin.

The Journal of cell biology ·Vol. 114 ·No. 3 ·1991-08-00 ·Pages 413-21

Brewer CB, Roth MG

Abstract

In the polarized kidney cell line MDCK, the influenza virus hemagglutinin (HA) has been well characterized as a model for apically sorted membrane glycoproteins. Previous work from our laboratory has shown that a single amino acid change in the cytoplasmic sequence of HA converts it from a protein that is excluded from coated pits to one that is efficiently internalized. Using trypsin or antibodies to mark protein on the surface, we have shown in MDCK cells that HA containing this mutation is no longer transported to the apical surface but instead is delivered directly to the basolateral plasma membrane. We propose that a cytoplasmic feature similar to an endocytosis signal can cause exclusive basolateral delivery.

MeSH Terms
Animals Antibodies/immunology Biological Transport Cell Line Cytoplasm/chemistry,metabolism Endocytosis Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/chemistry,metabolism Kinetics Methionine/metabolism Mutation Precipitin Tests Transfection Transferrin/metabolism Trypsin/metabolism Tyrosine/chemistry,immunology
Chemicals
Antibodies Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral Transferrin Tyrosine Methionine Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brewer C B
Department of Biochemistry, University of Texas Southwestern Medical Center, Dallas 75235-9038.
Roth M G
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44 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.114.3.413
Published
1991-08-00
Pages
413-21
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2289095
Subset
IM
Grants
NIGMS NIH HHS · GM37547 · United States
NIGMS NIH HHS · GM41050 · United States
External Links
PubMed source DOI: 10.1083/jcb.114.3.413 PMC Full Text View journal details
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