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PMID: 3279048 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Posttranslational oligomerization and cooperative acid activation of mixed influenza hemagglutinin trimers.

The Journal of cell biology ·Vol. 106 ·No. 3 ·1988-03-00 ·Pages 629-39

Boulay F, Doms RW, Webster RG, Helenius A

Abstract

The influenza virus hemagglutinin (HA) is a well-characterized integral membrane glycoprotein composed of three identical subunits. We have analyzed the formation of mixed trimers in cells expressing two different HA gene products. The results show efficient and essentially random assembly of functional hybrid trimers provided that the HAs are from the same HA subtype. Trimerization is thus a posttranslational event, and subunits are recruited randomly from a common pool of monomers in the endoplasmic reticulum. Mixed trimers were not observed between HAs derived from different subtypes, indicating that the trimerization event is sequence specific. Mixed trimers containing mutant subunits were, moreover, used to establish that the acid-induced conformational change involved in the membrane fusion activity of HA is a highly cooperative event.

MeSH Terms
Animals Cell Fusion/drug effects Cell Line Electrophoresis, Polyacrylamide Gel Fluorescent Antibody Technique Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral/genetics,metabolism Hydrogen-Ion Concentration Immunoassay Influenza A virus/genetics,metabolism Macromolecular Substances Protein Conformation Protein Processing, Post-Translational
Chemicals
Hemagglutinin Glycoproteins, Influenza Virus Hemagglutinins, Viral Macromolecular Substances
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Boulay F
Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510.
Doms R W
Webster R G
Helenius A
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.106.3.629
Published
1988-03-00
Pages
629-39
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2115101
Subset
IM
Grants
NIAID NIH HHS · AI-08831 · United States
NIAID NIH HHS · AI-18599 · United States
External Links
PubMed source DOI: 10.1083/jcb.106.3.629 PMC Full Text View journal details
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