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PMID: 7862157 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of the Runt domain-encoding PEBP2 alpha genes in T cells during thymic development.

Molecular and cellular biology ·Vol. 15 ·No. 3 ·1995-03-00 ·Pages 1662-70

Satake M, Nomura S, Yamaguchi-Iwai Y, Takahama Y, Hashimoto Y, Niki M, Kitamura Y, Ito Y

Abstract

The PEBP2 alpha A and PEBP2 alpha B genes encode the DNA-binding subunit of a murine transcription factor, PEBP2, which is implicated as a T-cell-specific transcriptional regulator. These two related genes share the evolutionarily conserved region encoding the Runt domain. PEBP2 alpha B is the murine counterpart of human AML1, which is located at the breakpoints of the 8;21 and 3;21 chromosome translocations associated with acute myeloid leukemia. Northern (RNA) blots of various adult mouse tissues revealed that the levels of expression of both genes were most prominent in the thymus. Furthermore, transcripts of PEBP2 alpha A and mouse AML1/PEBP2 alpha B were detected in T lymphocytes in the thymuses from day 16 embryos and newborns, as well as 4-week-old adult mice, by in situ hybridization. The expression of the genes persisted in peripheral lymph nodes of adult mice. The transcripts were detected in all the CD4- CD8-, CD4+ CD8+, CD4+ CD8-, and CD4- CD8+ cell populations. The results indicated that both genes are expressed in T cells throughout their development, supporting the notion that PEBP2 is a T-cell-specific transcription factor. Transcripts of mouse AML1/PEBP2 alpha B were also detected in day 12 fetal hematopoietic liver and in the bone marrow cells of newborn mice. The implication of mouse AML1/PEBP2 alpha B expression in hematopoietic cells other than those of T-cell lineage is discussed in relation to myeloid leukemogenesis.

Related Genes
AML1 PEBP2&agr PEBP2&agr A PEBP2&agr B
MeSH Terms
Acute Disease Aging/metabolism Animals Animals, Newborn Antisense Elements (Genetics) Base Sequence Biological Evolution Blotting, Northern Bone Marrow/metabolism CD4-Positive T-Lymphocytes/metabolism CD8-Positive T-Lymphocytes/metabolism Chromosomes, Human, Pair 21 Chromosomes, Human, Pair 3 Chromosomes, Human, Pair 8 Conserved Sequence Core Binding Factor Alpha 2 Subunit Core Binding Factor alpha Subunits DNA-Binding Proteins/biosynthesis,genetics Embryo, Mammalian Humans Leukemia, Myeloid/genetics Liver/metabolism Lymph Nodes/metabolism Male Mice Mice, Inbred C57BL Mice, Inbred ICR Mice, Nude Neoplasm Proteins/biosynthesis,genetics Organ Specificity Proto-Oncogene Proteins T-Lymphocytes/metabolism,physiology Thymus Gland/growth & development,metabolism Transcription Factor AP-2 Transcription Factors/biosynthesis,genetics Transcription, Genetic Translocation, Genetic
Chemicals
Antisense Elements (Genetics) Core Binding Factor Alpha 2 Subunit Core Binding Factor alpha Subunits DNA-Binding Proteins Neoplasm Proteins Proto-Oncogene Proteins RUNX1 protein, human Runx1 protein, mouse Transcription Factor AP-2 Transcription Factors
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Satake M
Department of Viral Oncology, Kyoto University, Japan.
Nomura S
Yamaguchi-Iwai Y
Takahama Y
Hashimoto Y
Niki M
Kitamura Y
Ito Y
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/MCB.15.3.1662
Published
1995-03-00
Pages
1662-70
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC230390
Subset
IM
External Links
PubMed source DOI: 10.1128/MCB.15.3.1662 PMC Full Text View journal details
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