Home LiteratureArticle Details
PMID: 7816621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

RAP1 stimulates single- to double-strand association of yeast telomeric DNA: implications for telomere-telomere interactions.

Nucleic acids research ·Vol. 22 ·No. 24 ·1994-12-11 ·Pages 5310-20

Gilson E, Müller T, Sogo J, Laroche T, Gasser SM

Abstract

Repressor Activator Protein 1 (RAP1) of Saccharomyces cerevisiae is an abundant nuclear protein implicated in telomere length maintenance, transactivation, and in the establishment of silent chromatin domains. The RAP1 binding site 5' of the yeast HIS4 gene is also a region of hyperrecombination in meiosis. We report here that as RAP1 binds its recognition consensus, it appears to untwist double-stranded DNA, which we detect as the introduction of a negative supercoil in circularization assays. Coincident with the RAP1-dependent untwisting, we observe stimulation of the association of a single-stranded yeast telomeric sequence with its homologous double-stranded sequence in a supercoiled plasmid. This unusual distortion of the DNA double helix by RAP1 may contribute to the RAP1-dependent enhancement of recombination rates and promote non-duplex strand interactions at telomeres.

MeSH Terms
Base Sequence DNA, Fungal/chemistry,metabolism DNA, Single-Stranded/metabolism DNA, Superhelical/metabolism DNA-Binding Proteins/isolation & purification,metabolism,physiology Molecular Sequence Data Nucleic Acid Conformation Plasmids/metabolism Saccharomyces cerevisiae/cytology,metabolism Telomere/metabolism Transcription Factors/isolation & purification,metabolism,physiology
Chemicals
DNA, Fungal DNA, Single-Stranded DNA, Superhelical DNA-Binding Proteins Transcription Factors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gilson E
I SREC (Swiss Institute for Experimental Cancer Research), Epalinges/Lausanne.
Müller T
Sogo J
Laroche T
Gasser S M
References (51)
51 references, click to expand
  1. Plasmid permutation vectors to monitor DNA bending.
    Nucleic Acids Res. 1987 Dec 10;15(23):10060 PMID: 3697072
  2. Interstitial telomeres are hotspots for illegitimate recombination with DNA molecules injected into the macronucleus of Paramecium primaurelia.
    EMBO J. 1992 Feb;11(2):725-32 PMID: 1311256
  3. Direct visualization of supercoiled DNA molecules in solution.
    EMBO J. 1990 Dec;9(13):4551-4 PMID: 2265618
  4. Association of poly(CA).poly(TG) DNA fragments into four-stranded complexes bound by HMG1 and 2.
    Science. 1994 Apr 15;264(5157):433-6 PMID: 8153633
  5. The chromosome end in yeast: its mosaic nature and influence on recombinational dynamics.
    Genetics. 1994 Mar;136(3):789-802 PMID: 8005434
  6. Closed circular DNA as a probe for protein-induced structural changes.
    Trends Biochem Sci. 1992 Jan;17(1):7-12 PMID: 1585455
  7. Further studies on chiasma distribution and interference in the human male.
    Ann Hum Genet. 1985 Jul;49(Pt 3):203-14 PMID: 4073834
  8. The beta subunit of Oxytricha telomere-binding protein promotes G-quartet formation by telomeric DNA.
    Cell. 1993 Sep 10;74(5):875-85 PMID: 8374954
  9. The yeast telomere-binding protein RAP1 binds to and promotes the formation of DNA quadruplexes in telomeric DNA.
    EMBO J. 1994 May 15;13(10):2411-20 PMID: 8194531
  10. Homologous recognition promoted by RecA protein via non-Watson-Crick bonds between identical DNA strands.
    Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6646-50 PMID: 8341681
  11. Transcription factors are required for the meiotic recombination hotspot at the HIS4 locus in Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1993 Jul 15;90(14):6621-5 PMID: 8341678
  12. The yeast KEM1 gene encodes a nuclease specific for G4 tetraplex DNA: implication of in vivo functions for this novel DNA structure.
    Cell. 1994 Jul 1;77(7):1083-92 PMID: 8020096
  13. Crystal structure of four-stranded Oxytricha telomeric DNA.
    Nature. 1992 Mar 12;356(6365):126-31 PMID: 1545863
  14. RAP1 is required for BAS1/BAS2- and GCN4-dependent transcription of the yeast HIS4 gene.
    Mol Cell Biol. 1991 Jul;11(7):3642-51 PMID: 1904543
  15. A yeast telomere binding activity binds to two related telomere sequence motifs and is indistinguishable from RAP1.
    Curr Genet. 1989 Oct;16(4):225-39 PMID: 2697465
  16. Strand separation required for initiation of replication at the chromosomal origin of E.coli is facilitated by a distant RNA--DNA hybrid.
    EMBO J. 1990 Jul;9(7):2341-8 PMID: 1694129
  17. Saccharomyces telomeres acquire single-strand TG1-3 tails late in S phase.
    Cell. 1993 Jan 15;72(1):51-60 PMID: 8422682
  18. An unusual DNA structure detected in a telomeric sequence under superhelical stress and at low pH.
    Nature. 1989 Jun 22;339(6226):634-7 PMID: 2733795
  19. Energetics of DNA twisting. I. Relation between twist and cyclization probability.
    J Mol Biol. 1983 Nov 15;170(4):957-81 PMID: 6315955
  20. Kinetic trapping of H-DNA by oligonucleotide binding.
    Nucleic Acids Res. 1992 Apr 25;20(8):1903-8 PMID: 1579491
  21. Molecular characterization of a pericentric inversion in mouse chromosome 8 implicates telomeres as promoters of meiotic recombination.
    Chromosoma. 1993 Jan;102(2):112-20 PMID: 8432192
  22. Recombination occurs during telomere formation in yeast.
    Nature. 1989 Feb 2;337(6206):429-33 PMID: 2536898
  23. Formation of joint DNA molecules by two eukaryotic strand exchange proteins does not require melting of a DNA duplex.
    J Biol Chem. 1989 Mar 25;264(9):5089-97 PMID: 2538473
  24. Genetic evidence that the meiotic recombination hotspot at the HIS4 locus of Saccharomyces cerevisiae does not represent a site for a symmetrically processed double-strand break.
    Genetics. 1993 May;134(1):5-19 PMID: 8514148
  25. Structure of the DNA gyrase-DNA complex as revealed by transient electric dichroism.
    J Mol Biol. 1987 Feb 5;193(3):555-69 PMID: 3035196
  26. S1 nuclease sensitivity of a double-stranded telomeric DNA sequence.
    Nucleic Acids Res. 1987 Aug 11;15(15):6273-92 PMID: 2819822
  27. Distribution of non-telomeric sites of the (TTAGGG)n telomeric sequence in vertebrate chromosomes.
    Chromosoma. 1990 Apr;99(1):3-10 PMID: 2340757
  28. DNA-binding protein RAP1 stimulates meiotic recombination at the HIS4 locus in yeast.
    Proc Natl Acad Sci U S A. 1991 Nov 1;88(21):9755-9 PMID: 1946399
  29. Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Nov;8(11):4642-50 PMID: 3062364
  30. Helix opening transitions in supercoiled DNA.
    Biochim Biophys Acta. 1992 May 7;1131(1):1-15 PMID: 1581350
  31. Structure and function of telomeres.
    Annu Rev Genet. 1989;23:579-604 PMID: 2694944
  32. Distortion of the DNA double helix by RAP1 at silencers and multiple telomeric binding sites.
    J Mol Biol. 1993 May 20;231(2):293-310 PMID: 8510148
  33. Formation of parallel four-stranded complexes by guanine-rich motifs in DNA and its implications for meiosis.
    Nature. 1988 Jul 28;334(6180):364-6 PMID: 3393228
  34. A "hot spot" of recombination coincides with an interstitial telomeric sequence in the Armenian hamster.
    Cytogenet Cell Genet. 1993;62(2-3):169-71 PMID: 8428518
  35. Position effect at S. cerevisiae telomeres: reversible repression of Pol II transcription.
    Cell. 1990 Nov 16;63(4):751-62 PMID: 2225075
  36. Functional reintroduction of human telomeres into mammalian cells.
    Proc Natl Acad Sci U S A. 1991 Aug 15;88(16):7006-10 PMID: 1871116
  37. Human telomeres: fusion and interstitial sites.
    Trends Genet. 1989 Oct;5(10):326-31 PMID: 2692239
  38. Rapid "footprinting" on supercoiled DNA.
    Proc Natl Acad Sci U S A. 1985 May;82(10):3078-81 PMID: 3889902
  39. A tetrameric DNA structure with protonated cytosine.cytosine base pairs.
    Nature. 1993 Jun 10;363(6429):561-5 PMID: 8389423
  40. A highly conserved repetitive DNA sequence, (TTAGGG)n, present at the telomeres of human chromosomes.
    Proc Natl Acad Sci U S A. 1988 Sep;85(18):6622-6 PMID: 3413114
  41. Targeted breakage of a human chromosome mediated by cloned human telomeric DNA.
    Nat Genet. 1992 Dec;2(4):283-7 PMID: 1303280
  42. Instability of simple sequence DNA in Saccharomyces cerevisiae.
    Mol Cell Biol. 1992 Jun;12(6):2749-57 PMID: 1588966
  43. Intramolecular folding of a fragment of the cytosine-rich strand of telomeric DNA into an i-motif.
    Nucleic Acids Res. 1994 May 11;22(9):1600-6 PMID: 8202359
  44. An alternative pathway for yeast telomere maintenance rescues est1- senescence.
    Cell. 1993 Apr 23;73(2):347-60 PMID: 8477448
  45. Formation of base triplets by non-Watson-Crick bonds mediates homologous recognition in RecA recombination filaments.
    Proc Natl Acad Sci U S A. 1994 Jun 21;91(13):6161-5 PMID: 8016131
  46. SIR3 and SIR4 proteins are required for the positioning and integrity of yeast telomeres.
    Cell. 1993 Nov 5;75(3):543-55 PMID: 8221893
  47. Telomeres and the functional architecture of the nucleus.
    Trends Cell Biol. 1993 Apr;3(4):128-34 PMID: 14731767
  48. Origin of human chromosome 2: an ancestral telomere-telomere fusion.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9051-5 PMID: 1924367
  49. Telomere-telomere recombination provides an express pathway for telomere acquisition.
    Nature. 1990 May 31;345(6274):456-8 PMID: 2111466
  50. Human telomeric C-strand tetraplexes.
    Nat Struct Biol. 1994 Feb;1(2):83-8 PMID: 7656022
  51. Parallel DNA triplexes, homologous recombination, and other homology-dependent DNA interactions.
    Cell. 1993 Apr 23;73(2):217-23 PMID: 8477443
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/22.24.5310
Published
1994-12-11
Pages
5310-20
Language
English
Region
England
NLM ID
0411011
PMCID
PMC332076
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/22.24.5310 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2