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PMID: 3062364 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Characterization of two telomeric DNA processing reactions in Saccharomyces cerevisiae.

Molecular and cellular biology ·Vol. 8 ·No. 11 ·1988-11-00 ·Pages 4642-50

Murray AW, Claus TE, Szostak JW

Abstract

We have investigated two reactions that occur on telomeric sequences introduced into Saccharomyces cerevisiae cells by transformation. The elongation reaction added repeats of the yeast telomeric sequence C1-3A to telomeric sequences at the end of linear DNA molecules. The reaction worked on the Tetrahymena telomeric sequence C4A2 and also on the simple repeat CA. The reaction was orientation specific: it occurred only when the GT-rich strand ran 5' to 3' towards the end of the molecule. Telomere elongation occurred by non-template-directed DNA synthesis rather than any type of recombination with chromosomal telomeres, because C1-3A repeats could be added to unrelated DNA sequences between the CA-rich repeats and the terminus of the transforming DNA. The elongation reaction was very efficient, and we believe that it was responsible for maintaining an average telomere length despite incomplete replication by template-directed DNA polymerase. The resolution reaction processed a head-to-head inverted repeat of telomeric sequences into two new telomeres at a frequency of 10(-2) per cell division.

MeSH Terms
Animals DNA Replication DNA, Fungal/genetics,metabolism Plasmids Repetitive Sequences, Nucleic Acid Saccharomyces cerevisiae/genetics,metabolism Tetrahymena/genetics
Chemicals
DNA, Fungal
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Murray A W
Department of Molecular Biology, Massachusetts General Hospital, Boston 02114.
Claus T E
Szostak J W
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.8.11.4642-4650.1988
Published
1988-11-00
Pages
4642-50
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365553
Subset
IM
Grants
NIGMS NIH HHS · GM32039 · United States
External Links
PubMed source DOI: 10.1128/mcb.8.11.4642-4650.1988 PMC Full Text View journal details
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