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PMID: 7815514 Published · ppublish English Journal Article

Two E2 binding sites alone are sufficient to function as the minimal origin of replication of human papillomavirus type 18 DNA.

Journal of virology ·Vol. 69 ·No. 2 ·1995-02-00 ·Pages 1319-23

Sverdrup F, Khan SA

Abstract

Replication of papillomaviruses requires an origin of replication and two virus-encoded proteins, E1 and E2. Using a transient replication assay for human papillomavirus type 18 (HPV-18) DNA, we have found that two adjacent sequences present within the origin of replication can independently support replication. The first, a 77-bp region, contains one E2 binding site (E2BS) and a 16-bp inverted repeat element that probably corresponds to the E1 binding site (E1BS). The other, an 81-bp region, includes two E2BS but lacks the putative E1BS. A synthetic 33-bp oligonucleotide containing two high-affinity E2BS was also found to function as an origin of replication. Replication of all these plasmids was absolutely dependent on the presence of the HPV-18 E1 and E2 proteins. The HPV-1a E1 and E2 proteins were also found to support replication of a plasmid containing the complete HPV-18 origin but failed to replicate a plasmid containing two E2BS alone. Our results suggest that the E2 protein can target E1 to the origin through the formation of an E1-E2 complex which is likely to be involved the initiation of replication.

MeSH Terms
Base Sequence Binding Sites DNA Replication Molecular Sequence Data Oncogene Proteins, Viral/physiology Papillomaviridae/genetics Plasmids Virus Replication
Chemicals
Oncogene Proteins, Viral oncogene protein E1, Human papillomavirus type 18 oncogene protein E2, Human papillomavirus type 18
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sverdrup F
Department of Molecular Genetics and Biochemistry, University of Pittsburgh School of Medicine, Pennsylvania 15261.
Khan S A
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.2.1319-1323.1995
Published
1995-02-00
Pages
1319-23
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC188713
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.69.2.1319-1323.1995 PMC Full Text View journal details
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