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PMID: 7814605 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of smooth muscle cell scavenger receptor expression in vivo by atherogenic diets and in vitro by cytokines.

The Journal of clinical investigation ·Vol. 95 ·No. 1 ·1995-01-00 ·Pages 122-33

Li H, Freeman MW, Libby P

Abstract

Scavenger receptor (ScR)-mediated uptake of modified lipoproteins may contribute to the transformation of smooth muscle cells into lipid-laden foam cells during atherogenesis. This study examined the in vivo expression of ScRs in aortas, with or without balloon injury, taken from hypercholesterolemic or normocholesterolemic rabbits. Numerous intimal cells in the rabbit aortic lesions expressed ScRs as detected by immunocytochemical staining with a goat anti-rabbit ScR antibody. Single immunostaining for cell identification markers in serial sections, as well as double staining, confirmed the expression of ScRs by both intimal smooth muscle cells and macrophages. To explore potential inducers of ScR expression by smooth muscle cells in vivo, we studied the regulation of ScR expression in vitro by cytokines known to be present in atherosclerotic lesions. Tumor necrosis factor-alpha (TNF-alpha) or interferon-gamma (IFN-gamma) increased ScR mRNA levels, protein expression, and AcLDL degradative activity in cultured rabbit aortic smooth muscle cells. The induction of ScR expression in intimal smooth muscle cells in vivo could be a useful marker of smooth muscle cell activation during atherogenesis and may contribute to foam cell formation by this cell type following balloon injury and/or hypercholesterolemia. Cytokines, such as TNF-alpha or IFN-gamma, may stimulate some of the phenotypic changes that characterize the alteration in gene expression of intimal smooth muscle cells in rabbit atherosclerotic lesions.

MeSH Terms
Animals Aorta/injuries,metabolism,pathology Arteriosclerosis/metabolism Cell Differentiation Cells, Cultured Cytokines/pharmacology Diet, Atherogenic Foam Cells Gene Expression Regulation Hypercholesterolemia/metabolism Immunohistochemistry Interferon-gamma/pharmacology Lipoproteins, LDL/metabolism Male Membrane Proteins Muscle, Smooth, Vascular/cytology,metabolism Rabbits Receptors, Immunologic/isolation & purification,metabolism Receptors, Lipoprotein Receptors, Scavenger Scavenger Receptors, Class B Tumor Necrosis Factor-alpha/pharmacology Tunica Intima/metabolism
Chemicals
Cytokines Lipoproteins, LDL Membrane Proteins Receptors, Immunologic Receptors, Lipoprotein Receptors, Scavenger Scarb1 protein, mouse Scavenger Receptors, Class B Tumor Necrosis Factor-alpha acetyl-LDL Interferon-gamma
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Li H
Department of Pathology, Brigham and Women's Hospital, Boston, Massachusetts 02115.
Freeman M W
Libby P
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI117628
Published
1995-01-00
Pages
122-33
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC295387
Subset
IM
Grants
NHLBI NIH HHS · HL-45098 · United States
NHLBI NIH HHS · HL-47840 · United States
NHLBI NIH HHS · P0-1-HL-48743 · United States
External Links
PubMed source DOI: 10.1172/JCI117628 PMC Full Text View journal details
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