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PMID: 7790025 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Superantigen-induced peripheral T-cell deletion: the effects of chemical modification of antigen-presenting cells, interleukin-4 and glucocorticoid hormones.

Immunology ·Vol. 84 ·No. 4 ·1995-04-00 ·Pages 528-35

Ayroldi E, Cannarile L, D'Adamio F, Riccardi C

Abstract

Experiments were performed to evaluate the role of antigen-presenting cells (APC) and the effect of interleukin-4 (IL-4) and glucocorticoid hormone (GCH) exposure on the in vitro deletion of CD4+ CD8- and CD8+ CD4- T cells by staphylococcal enterotoxin B (SEB). APC fixation with the chemical cross-linker 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide (ECDI) inhibited their capacity to induce SEB-specific deletion of mature T lymphocytes. Deletion was not influenced by treatment with anti-CD28 antibodies, which modulate T-cell activation. However, it was augmented by IL-4, known to counteract anti-CD3- and GCH-induced thymocyte apoptosis, and was inhibited by dexamethasone (DEX). These results indicate that metabolically active APC are required for deletion of antigen-specific mature T cells and suggest that IL-4 and GCH can modulate this phenomenon in vitro.

MeSH Terms
Animals Antigen-Presenting Cells/drug effects,immunology Apoptosis/drug effects,immunology CD28 Antigens/immunology Cell Division/immunology Cells, Cultured Dendritic Cells/immunology Dexamethasone/pharmacology Enterotoxins/immunology Ethyldimethylaminopropyl Carbodiimide/pharmacology Interleukin-4/immunology Mice Mice, Inbred C3H Superantigens/immunology T-Lymphocyte Subsets/immunology
Chemicals
CD28 Antigens Enterotoxins Superantigens Interleukin-4 enterotoxin B, staphylococcal Dexamethasone Ethyldimethylaminopropyl Carbodiimide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Ayroldi E
Department of Clinical Medicine, Pathology and Pharmacology, Perugia University Medical School, Italy.
Cannarile L
D'Adamio F
Riccardi C
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Article Info
Journal
Immunology
Abbr.
Immunology
ISSN
0019-2805
Published
1995-04-00
Pages
528-35
Language
English
Region
England
NLM ID
0374672
PMCID
PMC1415147
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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