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PMID: 7716245 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

The effects of mastoparan on the carrot cell plasma membrane polyphosphoinositide phospholipase C.

Plant physiology ·Vol. 107 ·No. 3 ·1995-03-00 ·Pages 845-56

Cho MH, Tan Z, Erneux C, Shears SB, Boss WF

Abstract

When [3H]inositol-labeled carrot (Daucus carota L.) cells were treated with 10 or 25 microM wasp venom peptide mastoparan or the active analog Mas-7 there was a rapid loss of more than 70% of [3H]phosphatidylinositol-4-monophosphate (PIP) and [3H]phosphatidylinositol-4,5-bisphosphate (PIP2) and a 3- and 4-fold increase in [3H]inositol-1,4-P2 and [3H]inositol-1,4,5-P3, respectively. The identity of [3H]inositol-1,4,5-P3 was confirmed by phosphorylation with inositol-1,4,5-P3 3-kinase and co-migration with inositol-1,3,4,5-P4. The changes in phosphoinositides were evident within 1 min. The loss of [3H]PIP was evident only when cells were treated with the higher concentrations (10 and 25 microM) of mastoparan or Mas-7. At 1 microM Mas-7, [3H]PIP increased. The inactive mastoparan analog Mas-17 had little or no effect on [3H]PIP or [3H]PIP2 hydrolysis in vivo. Neomycin (100 microM) inhibited the uptake of Mas-7 and thereby inhibited the Mas-7-stimulated hydrolysis of [3H]PIP and [3H]PIP2. Plasma membranes isolated from mastoparan-treated cells had increased PIP-phospholipase C (PLC) activity. However, when Mas-7 was added to isolated plasma membranes from control cells, it had no effect on PIP-PLC activity at low concentrations and inhibited PIP-PLC at concentrations greater than 10 microM. In addition, guanosine-5'-O-(3-thiotriphosphate) had no effect on the PIP-PLC activity when added to plasma membranes isolated from either the Mas-7-treated or control cells. The fact that Mas-7 did not stimulate PIP-PLC activity in vitro indicated that the Mas-7-induced increase in PIP-PLC in vivo required a factor that was lost from the membrane during isolation.

MeSH Terms
Amino Acid Sequence Calcium Channel Blockers/pharmacology Cell Membrane/drug effects,enzymology Cells, Cultured Daucus carota/cytology,drug effects,enzymology Enzyme Activation Guanosine 5'-O-(3-Thiotriphosphate)/pharmacology Inositol Phosphates/metabolism Intercellular Signaling Peptides and Proteins Lanthanum/pharmacology Molecular Sequence Data Neomycin/pharmacology Peptides Phosphoinositide Phospholipase C Phosphoric Diester Hydrolases/drug effects Wasp Venoms/pharmacology
Chemicals
Calcium Channel Blockers Inositol Phosphates Intercellular Signaling Peptides and Proteins Peptides Wasp Venoms Guanosine 5'-O-(3-Thiotriphosphate) Lanthanum mastoparan Phosphoric Diester Hydrolases Phosphoinositide Phospholipase C Neomycin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Cho M H
Department of Botany, North Carolina State University, Raleigh 27695-7612.
Tan Z
Erneux C
Shears S B
Boss W F
References (52)
52 references, click to expand
  1. Inositol 1,4,5,6-tetrakisphosphate is phosphorylated in rat liver by a 3-kinase that is distinct from inositol 1,4,5-trisphosphate 3-kinase.
    J Biol Chem. 1994 Feb 11;269(6):4337-42 PMID: 8308002
  2. Polyphosphoinositide Phospholipase C in Plasma Membranes of Wheat (Triticum aestivum L.) : Orientation of Active Site and Activation by Ca and Mg.
    Plant Physiol. 1992 Nov;100(3):1296-303 PMID: 16653120
  3. Inositol lipids in cellular signalling mechanisms.
    Trends Biochem Sci. 1992 Aug;17(8):274-6 PMID: 1412699
  4. Mechanisms of mastoparan-stimulated surfactant secretion from isolated pulmonary alveolar type 2 cells.
    J Biol Chem. 1991 Apr 15;266(11):6859-65 PMID: 1849893
  5. Wind-induced plant motion immediately increases cytosolic calcium.
    Proc Natl Acad Sci U S A. 1992 Jun 1;89(11):4967-71 PMID: 11536497
  6. Association of Phosphatidylinositol Kinase, Phosphatidylinositol Monophosphate Kinase, and Diacylglycerol Kinase with the Cytoskeleton and F-Actin Fractions of Carrot (Daucus carota L.) Cells Grown in Suspension Culture : Response to Cell Wall-Degrading Enzymes.
    Plant Physiol. 1992 Dec;100(4):2116-20 PMID: 16653250
  7. Mastoparan stimulates exocytosis at a Ca(2+)-independent late site in stimulus-secretion coupling. Studies with the RINm5F beta-cell line.
    J Biol Chem. 1993 Nov 5;268(31):23297-306 PMID: 8226853
  8. Polyamines and neomycin inhibit the purified plasma-membrane Ca2+ pump by interacting with associated polyphosphoinositides.
    Biochem J. 1989 Aug 1;261(3):1055-8 PMID: 2552987
  9. Inositol-lipid-specific phospholipase C isoenzymes and their differential regulation by receptors.
    Biochem J. 1992 Nov 15;288 ( Pt 1):1-14 PMID: 1332691
  10. Identification of an ARF type low molecular mass GTP-binding protein in pea (Pisum sativum).
    Biochem Biophys Res Commun. 1993 Jun 30;193(3):809-13 PMID: 8323556
  11. Neomycin inhibits platelet functions and inositol phospholipid metabolism upon stimulation with thrombin, but not with ionomycin or 12-O-tetradecanoyl-phorbol 13-acetate.
    Eur J Biochem. 1988 Oct 15;177(1):219-23 PMID: 2846297
  12. Signal transduction and the actin cytoskeleton: the roles of MARCKS and profilin.
    Trends Biochem Sci. 1992 Oct;17(10):438-43 PMID: 1455513
  13. Mastoparan, a novel mitogen for Swiss 3T3 cells, stimulates pertussis toxin-sensitive arachidonic acid release without inositol phosphate accumulation.
    J Cell Biol. 1991 May;113(4):943-50 PMID: 1709171
  14. Inositol phospholipids activate plasma membrane ATPase in plants.
    Biochem Biophys Res Commun. 1989 Aug 15;162(3):1295-301 PMID: 2548494
  15. Plant Phosphoinositides and Intracellular Signaling.
    Plant Physiol. 1993 Jul;102(3):705-709 PMID: 12231858
  16. Purification of polyphosphoinositides by chromatography on immobilized neomycin.
    J Lipid Res. 1978 Nov;19(8):1063-7 PMID: 215685
  17. Regulation of phospholipase C by G proteins.
    Trends Biochem Sci. 1992 Dec;17(12):502-6 PMID: 1335185
  18. Inositol phospholipid metabolism may trigger flagellar excision in Chlamydomonas reinhardtii.
    J Cell Biol. 1992 Feb;116(3):737-44 PMID: 1309818
  19. Mastoparan induces oscillations of cytosolic Ca2+ in rat pancreatic acinar cells.
    Biochem Biophys Res Commun. 1991 May 31;177(1):159-65 PMID: 2043103
  20. Communication between two globular domains of calmodulin in the presence of mastoparan or caldesmon fragment. Ca2+ binding and 1H NMR.
    J Biol Chem. 1987 Aug 15;262(23):10951-4 PMID: 3611098
  21. Characterization of a G-protein-regulated outward K+ current in mesophyll cells of vicia faba L.
    Proc Natl Acad Sci U S A. 1993 Jan 1;90(1):262-6 PMID: 8419932
  22. Diversity of G proteins in signal transduction.
    Science. 1991 May 10;252(5007):802-8 PMID: 1902986
  23. Characterization of a Polyphosphoinositide Phospholipase C from the Plasma Membrane of Avena sativa.
    Plant Physiol. 1989 Dec;91(4):1275-9 PMID: 16667176
  24. Neomycin inhibits inositol phosphate formation in human platelets stimulated by thrombin but not other agonists.
    FEBS Lett. 1986 Oct 20;207(1):53-7 PMID: 3770193
  25. Changes in the levels of inositol phosphates after agonist-dependent hydrolysis of membrane phosphoinositides.
    Biochem J. 1983 May 15;212(2):473-82 PMID: 6309146
  26. Regulation of polyphosphoinositide-specific phospholipase C activity by purified Gq.
    Science. 1991 Feb 15;251(4995):804-7 PMID: 1846707
  27. Phosphoinositide hydrolysis in permeabilized SH-SY5Y human neuroblastoma cells is inhibited by mastoparan.
    FEBS Lett. 1989 Apr 24;247(2):341-4 PMID: 2541020
  28. Evidence for G-Protein Regulation of Inward K+ Channel Current in Guard Cells of Fava Bean.
    Plant Cell. 1991 Sep;3(9):1037-1044 PMID: 12324626
  29. Effects of mastoparan on catecholamine release from chromaffin cells.
    FEBS Lett. 1989 Apr 24;247(2):239-41 PMID: 2714434
  30. Small GTP-binding proteins in vesicular transport.
    Trends Biochem Sci. 1990 Dec;15(12):473-7 PMID: 2077687
  31. Phosphatidylinositol specific phospholipase C of plant stems : membrane associated activity concentrated in plasma membranes.
    Plant Physiol. 1987 Dec;85(4):1151-5 PMID: 16665820
  32. Identification of Ca2+-stimulated polyphosphoinositide phospholipase C in isolated plant plasma membranes.
    FEBS Lett. 1987 Oct 19;223(1):87-91 PMID: 2822482
  33. Mastoparan promotes exocytosis and increases intracellular cyclic AMP in human platelets. Evidence for the existence of a Ge-like mechanism of secretion.
    Biochem J. 1992 Jan 15;281 ( Pt 2):465-72 PMID: 1310599
  34. Inositol phosphates and cell signalling.
    Nature. 1989 Sep 21;341(6239):197-205 PMID: 2550825
  35. Preparation of solutions with free calcium concentration in the nanomolar range using 1,2-bis(o-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid.
    Anal Biochem. 1991 Feb 15;193(1):61-71 PMID: 1904204
  36. Mapping of the mastoparan-binding site on G proteins. Cross-linking of [125I-Tyr3,Cys11]mastoparan to Go.
    J Biol Chem. 1991 Jul 5;266(19):12655-61 PMID: 1905730
  37. Phosphatidylinositol-4,5-bisphosphate may antecede diacylglycerol as activator of protein kinase C.
    Biochem Biophys Res Commun. 1988 Aug 30;155(1):18-23 PMID: 2843176
  38. Regulation of phosphatidylinositol 4-kinase by the protein activator PIK-A49. Activation requires phosphorylation of PIK-A49.
    J Biol Chem. 1994 Feb 4;269(5):3852-7 PMID: 8106430
  39. Insulin releasing effects of mastoparan and amphiphilic substance P receptor antagonists on RINm5F insulinoma cells.
    Mol Cell Biochem. 1992 Feb 12;109(2):133-8 PMID: 1378173
  40. Intracellular translocation of a 28 kDa GTP-binding protein during osmotic shock-induced cell volume regulation in Dunaliella salina.
    Biochim Biophys Acta. 1993 Oct 7;1179(1):11-22 PMID: 8399350
  41. Two distinct, calcium-mediated, signal transduction pathways can trigger deflagellation in Chlamydomonas reinhardtii.
    J Cell Biol. 1994 Mar;124(5):807-15 PMID: 8120101
  42. Phosphatidylinositol 4,5-bisphosphate competitively inhibits phorbol ester binding to protein kinase C.
    Biochemistry. 1989 Jun 13;28(12):4952-6 PMID: 2548596
  43. Phosphatidylinositol phosphodiesterase in higher plants.
    Biochem J. 1980 Oct 15;192(1):279-83 PMID: 6272700
  44. Inositol-containing lipids in higher plants.
    Prog Lipid Res. 1992;31(1):53-63 PMID: 1641396
  45. Mastoparan, a wasp venom, stimulates insulin release by pancreatic islets through pertussis toxin sensitive GTP-binding protein.
    Biochem Biophys Res Commun. 1989 Feb 15;158(3):712-6 PMID: 2493246
  46. Facilitation of phospholipase A2 activity by mastoparans, a new class of mast cell degranulating peptides from wasp venom.
    J Biol Chem. 1983 Nov 25;258(22):13697-702 PMID: 6643447
  47. Activation of exocytosis by the heterotrimeric G protein Gi3.
    Science. 1993 Dec 3;262(5139):1569-72 PMID: 7504324
  48. Inhibition of polyphosphoinositide phosphodiesterase by aminoglycoside antibiotics.
    Neurochem Res. 1985 Aug;10(8):1019-24 PMID: 2997640
  49. The profilin multigene family of maize: differential expression of three isoforms.
    Plant J. 1993 Oct;4(4):631-41 PMID: 8252067
  50. Activation of the beta 1 isozyme of phospholipase C by alpha subunits of the Gq class of G proteins.
    Nature. 1991 Apr 11;350(6318):516-8 PMID: 1707501
  51. Regulation of Gi and Go by mastoparan, related amphiphilic peptides, and hydrophobic amines. Mechanism and structural determinants of activity.
    J Biol Chem. 1990 Aug 25;265(24):14176-86 PMID: 2117607
  52. Cloning and expression in Escherichia coli of a rat brain cDNA encoding a Ca2+/calmodulin-sensitive inositol 1,4,5-trisphosphate 3-kinase.
    Biochem J. 1990 Nov 15;272(1):107-12 PMID: 2176078
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.107.3.845
Published
1995-03-00
Pages
845-56
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC157201
Subset
IM
External Links
PubMed source DOI: 10.1104/pp.107.3.845 PMC Full Text View journal details
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