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PMID: 7650177 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Geographic distribution of human rotavirus VP4 genotypes and VP7 serotypes in five South African regions.

Journal of clinical microbiology ·Vol. 33 ·No. 6 ·1995-06-00 ·Pages 1516-9

Steele AD, van Niekerk MC, Mphahlele MJ

Abstract

The rotavirus outer capsid proteins elicit the production of neutralizing antibodies and are known to play a role in inducing resistance to disease. In this study, cDNA probes directed at the six most common human rotavirus VP7 serotypes (G1 to G4, G8, and G9) and five human rotavirus VP4 genotypes (P4, P6, P8, P9, and P10) were utilized. Hybridization analysis of 572 human rotavirus strains collected from five regions in South Africa was performed to determine the distribution of the VP7 serotypes and VP4 genotypes in nature. VP7 serotype G1 was identified most frequently, occurring in 51% of the rotavirus strains tested. VP7 serotypes G2 and G4 occurred in similar numbers, although their distribution varied regionally. Few serotype G3 strains and no G8 or G9 strains were identified. The P8 VP4 genotype occurred most frequently overall (66%), and the P4 genotype was detected next most frequently. The P6 genotype was identified in 28 symptomatically infected neonates and in 8 symptomatic infants. Few P9 strains were identified. The potential for reassortment events was demonstrated by dual infections with different viruses.

MeSH Terms
Antigens, Viral Capsid/genetics,immunology Capsid Proteins Child, Preschool DNA, Complementary Genotype Humans Polymerase Chain Reaction/statistics & numerical data Rotavirus/classification,genetics,isolation & purification Rotavirus Infections/epidemiology,virology Sensitivity and Specificity Serotyping South Africa/epidemiology
Chemicals
Antigens, Viral Capsid Proteins DNA, Complementary VP4 protein, Rotavirus VP7 protein, Rotavirus
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Steele A D
van Niekerk M C
Mphahlele M J
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34 references, click to expand
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.33.6.1516-1519.1995
Published
1995-06-00
Pages
1516-9
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC228206
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.33.6.1516-1519.1995 PMC Full Text View journal details
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