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PMID: 2984247 Published · ppublish English Journal Article

Serotypic characterization of rotaviruses derived from asymptomatic human neonatal infections.

Journal of clinical microbiology ·Vol. 21 ·No. 3 ·1985-03-00 ·Pages 425-30

Hoshino Y, Wyatt RG, Flores J, Midthun K, Kapikian AZ

Abstract

Nineteen rotavirus strains derived from asymptomatic neonates (seven from England, five from Australia, two from Venezuela, and five from Sweden) were successfully cultivated in primary African green monkey kidney cell cultures, serotyped by plaque reduction neutralization tests, subgrouped by indirect enzyme-linked immunosorbent assay, and electropherotyped by polyacrylamide gel electrophoresis. All 19 strains were shown to fall into one of the four known human serotypes; serotype 1 (all Venezuelan strains), serotype 2 (all Swedish strains), serotype 3 (all Australian strains), or serotype 4 (all English strains). Hyperimmune guinea pig serum raised against the Venezuelan strain (M37) neutralized not only serotype 1 (strain Wa) but also serotype 4 (strain St. Thomas no. 3) viruses to a similar degree. The English, Australian, and Venezuelan isolates were found to belong to subgroup 2, and the Swedish strains were subgroup 1 viruses. The potential importance of these rotaviruses obtained from neonates as possible vaccine candidates is discussed.

MeSH Terms
Antigens, Viral/analysis Electrophoresis, Polyacrylamide Gel Humans Infant, Newborn Nucleic Acid Hybridization RNA, Double-Stranded/analysis RNA, Viral/analysis Rotavirus/classification,genetics,pathogenicity Rotavirus Infections/microbiology Serotyping Viral Plaque Assay Virulence
Chemicals
Antigens, Viral RNA, Double-Stranded RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Hoshino Y
Wyatt R G
Flores J
Midthun K
Kapikian A Z
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Article Info
Journal
Journal of clinical microbiology
Abbr.
J Clin Microbiol
ISSN
0095-1137
DOI
10.1128/jcm.21.3.425-430.1985
Published
1985-03-00
Pages
425-30
Language
English
Region
United States
NLM ID
7505564
PMCID
PMC271678
Subset
IM
External Links
PubMed source DOI: 10.1128/jcm.21.3.425-430.1985 PMC Full Text View journal details
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