New opportunities for development of safe, effective live virus vaccines.

The Yale journal of biology and medicine ·Vol. 55 ·No. 3-4 ·1982-00-00 ·Pages 361-7

Chanock RM

Abstract

Effective vaccines are not available for most viral diseases. This situation may soon change when the full force of contemporary molecular biology is applied to immunoprophylaxis. In certain viral diseases, particularly those affecting the respiratory and gastrointestinal tracts, live attenuated vaccines are needed to confer effective protection. Until now the major obstacle to success has been genetic instability. It may be possible to construct stable, satisfactorily attenuated mutants by cloning viral DNA or RNA and then subjecting the cloned DNA to enzyme surgery to create viable deletion mutations. Modified cloned DNA derived from positive-strand viral RNA could then be transferred back into its virus by transfection of cells. Conversion of mutant cloned DNA into negative-strand RNA and transfer into its virus will require a more elaborate type of rescue.

MeSH Terms

Adenoviruses, Human/genetics,immunology Cloning, Molecular Genes, Viral Humans Influenza A virus/genetics,pathogenicity Influenza Vaccines Mutation Poliovirus/genetics Recombination, Genetic Rotavirus/genetics,immunology Transfection Vaccines, Attenuated Viral Vaccines

Chemicals

Influenza Vaccines Vaccines, Attenuated Viral Vaccines

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Chanock R M

References (13)

13 references, click to expand

Immunization by selective infection with type 4 adenovirus grown in human diploid tissue culture. II. specific protective effect against epidemic disease.
JAMA. 1966 Feb 7;195(6):453-9 PMID: 4285433
Rotaviral immunity in gnotobiotic calves: heterologous resistance to human virus induced by bovine virus.
Science. 1979 Feb 9;203(4380):548-50 PMID: 216077
Isolation of adenovirus type 5 host range deletion mutants defective for transformation of rat embryo cells.
Cell. 1979 Jul;17(3):683-9 PMID: 476833
Human rotavirus type 2: cultivation in vitro.
Science. 1980 Jan 11;207(4427):189-91 PMID: 6243190
Cloning DNA sequences from influenza viral RNA segments.
Proc Natl Acad Sci U S A. 1980 Jan;77(1):210-4 PMID: 6244544
Conservation and variation in Orthopoxvirus genome structure.
J Gen Virol. 1979 Dec;45(3):683-701 PMID: 232137
Variable defectiveness for lytic growth of the dl 54/59 mutants of simian virus 40.
J Virol. 1980 Mar;33(3):1208-10 PMID: 6245271
Rescue of noncultivatable human rotavirus by gene reassortment during mixed infection with ts mutants of a cultivatable bovine rotavirus.
Proc Natl Acad Sci U S A. 1981 Jan;78(1):420-4 PMID: 6264442
Identification of point mutations in the genome of the poliovirus Sabin vaccine LSc 2ab, and catalogue of RNase T1- and RNase A-resistant oligonucleotides of poliovirus type 1 (Mahoney) RNA.
Virology. 1981 Jul 15;112(1):217-27 PMID: 6264681
Growth and persistence of polyoma early region deletion mutants in mice.
J Virol. 1981 Sep;39(3):958-62 PMID: 6270362
Functional expression in primate cells of cloned DNA coding for the hemagglutinin surface glycoprotein of influenza virus.
Proc Natl Acad Sci U S A. 1981 Sep;78(9):5488-92 PMID: 6272305
Cloned poliovirus complementary DNA is infectious in mammalian cells.
Science. 1981 Nov 20;214(4523):916-9 PMID: 6272391
Isolation of human rotavirus in cell cultures: brief report.
Arch Virol. 1981;69(2):155-60 PMID: 6171239

Article Info

Journal

The Yale journal of biology and medicine

Abbr.

Yale J Biol Med

ISSN

0044-0086

Published

1982-00-00

Pages

361-7

Language

English

Region

United States

NLM ID

0417414

PMCID

PMC2596472

Subset

External Links

PubMed source PMC Full Text View journal details

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