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PMID: 7592492 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Evidence that the immunity protein inactivates colicin 5 immediately prior to the formation of the transmembrane channel.

Journal of bacteriology ·Vol. 177 ·No. 23 ·1995-12-00 ·Pages 6966-72

Pilsl H, Braun V

Abstract

Determination and analysis of the nucleotide sequences of the activity, immunity, and lysis genes of colicin 5 assigned colicin 5 to the subclass of pore-forming colicins to which colicins 10, E1, Ia, Ib, and K belong. Mutational analysis of colicin 5 and exchange of DNA fragments between the most closely related colicins, colicins 5 and 10, and between their immunity proteins localized the regions that determine the reaction specificity between colicin 5 and its immunity protein to residues 405 to 424 of colicin 5, the region corresponding to the amphiphilic alpha-helix 6 of the similar colicins E1 and Ia. The specificity-conferring residues 55 to 58 and 68 to 75 of the immunity protein were localized in the cytoplasmic loop and the inner leaflet of the cytoplasmic membrane. The localization of the reactive regions of the immunity protein and the colicin close to the inner side of the cytoplasmic membrane suggests that the immunity protein inactivates colicin 5 shortly before the lethal colicin pores in the cytoplasmic membrane are opened.

MeSH Terms
Amino Acid Sequence Bacterial Proteins/metabolism Bacteriocin Plasmids/genetics Base Sequence Binding Sites Cell Membrane/metabolism Cloning, Molecular Colicins/genetics,metabolism DNA Mutational Analysis Escherichia coli/genetics Escherichia coli Proteins Genes, Bacterial Ion Channels/genetics,metabolism Models, Molecular Molecular Sequence Data Protein Binding Protein Conformation Sequence Homology, Amino Acid
Chemicals
Bacterial Proteins Col10 protein, E coli Colicins Escherichia coli Proteins Imm protein, E coli Ion Channels colicin 5 protein, E coli colicin immunity proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Pilsl H
Universität Tübingen, Germany.
Braun V
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35 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.23.6966-6972.1995
Published
1995-12-00
Pages
6966-72
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177567
Subset
IM
Databases
GENBANK
X87835
External Links
PubMed source DOI: 10.1128/jb.177.23.6966-6972.1995 PMC Full Text View journal details
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