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PMID: 6953432 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Nucleotide sequence of the structural gene for colicin E1 and predicted structure of the protein.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 9 ·1982-05-00 ·Pages 2827-31

Yamada M, Ebina Y, Miyata T, Nakazawa T, Nakazawa A

Abstract

We have determined the nucleotide sequence of the structural gene for colicin E1, which consists of 1,566 base pairs. The amino acid sequence (522 residues) of the protein was derived from the DNA sequence, and the molecular weight was calculated to be 57,279. From the analysis of the predicted secondary structure, there appear to be three consecutive long alpha-helices in the NH2-terminal half of the polypeptide, spanning 40, 100, and 35 amino acid residues. In addition, there is a polypeptide region near the COOH terminus that shows homology to the NH2-terminal signal portions of outer membrane lipoprotein in Escherichia coli and beta-lactamase in Bacillus licheniformis. Most of the homologous amino acids are located in the region where either alpha-helix or beta-sheet would be expected to occur, as determined from the amino acid sequence. These characteristics of the predicted protein structure might correspond to properties of colicin E1 as an ionophore in its antimicrobial action and also as an exported protein during its induced synthesis.

MeSH Terms
Amino Acid Sequence Base Sequence Colicins/genetics DNA, Bacterial/genetics Genes Genes, Bacterial Protein Conformation Protein Precursors/metabolism Structure-Activity Relationship
Chemicals
Colicins DNA, Bacterial Protein Precursors
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Yamada M
Ebina Y
Miyata T
Nakazawa T
Nakazawa A
References (37)
37 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.9.2827
Published
1982-05-00
Pages
2827-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346299
Subset
IM
Databases
GENBANK
J01563
External Links
PubMed source DOI: 10.1073/pnas.79.9.2827 PMC Full Text View journal details
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