Home LiteratureArticle Details
PMID: 7592367 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

bvg Repression of alcaligin synthesis in Bordetella bronchiseptica is associated with phylogenetic lineage.

Journal of bacteriology ·Vol. 177 ·No. 21 ·1995-11-00 ·Pages 6058-63

Giardina PC, Foster LA, Musser JM, Akerley BJ, Miller JF, Dyer DW

Abstract

Recent studies have shown that Bordetella bronchiseptica utilizes a siderophore-mediated transport system for acquisition of iron from the host iron-binding proteins lactoferrin and transferrin. We recently identified the B. bronchiseptica siderophore as alcaligin, which is also produced by B. pertussis. Alcaligin production by B. bronchiseptica is repressed by exogenous iron, a phenotype of other microbes that produce siderophores. In this study, we report that alcaligin production by B. bronchiseptica RB50 and GP1SN was repressed by the Bordetella global virulence regulator, bvg, in addition to being Fe repressed. Modulation of bvg locus expression with 50 mM MgSO4 or inactivation of bvg by deletion allowed strain RB50 to produce alcaligin. In modulated organisms, siderophore production remained Fe repressed. These observations contrasted with our previous data indicating that alcaligin production by B. bronchiseptica MBORD846 and B. pertussis was repressed by Fe but bvg independent. Despite bvg repression of alcaligin production, strain RB50 was still able to acquire Fe from purified alcaligin, suggesting that expression of the bacterial alcaligin receptor was not repressed by bvg. We tested 114 B. bronchiseptica strains and found that bvg repression of alcaligin production was strongly associated with Bordetella phylogenetic lineage and with host species from which the organisms were isolated.

MeSH Terms
Bacterial Proteins/genetics Bordetella bronchiseptica/classification,genetics Gene Expression Regulation, Bacterial Hydroxamic Acids Iron/metabolism,pharmacology Lactoferrin/metabolism Magnesium Sulfate/pharmacology Phylogeny Siderophores/biosynthesis Species Specificity Trans-Activators/genetics
Chemicals
Bacterial Proteins Bvg accessory factor protein, Bordetella pertussis Hydroxamic Acids Siderophores Trans-Activators alcaligin Magnesium Sulfate Iron Lactoferrin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Giardina P C
Department of Microbiology and Immunology, University of Oklahoma Health Sciences Center, Oklahoma City 73190, USA.
Foster L A
Musser J M
Akerley B J
Miller J F
Dyer D W
References (48)
48 references, click to expand
  1. Characterization of vir-activated TnphoA gene fusions in Bordetella pertussis.
    Infect Immun. 1991 Sep;59(9):3273-9 PMID: 1652562
  2. Pneumonia caused by Bordetella bronchiseptica in a patient with a thoracic trauma.
    Infection. 1991 Jan-Feb;19(1):46-8 PMID: 2013509
  3. Siderophore production and membrane alterations by Bordetella pertussis in response to iron starvation.
    Infect Immun. 1992 Jan;60(1):117-23 PMID: 1309510
  4. A vir-repressed gene of Bordetella pertussis is required for virulence.
    Infect Immun. 1992 Feb;60(2):571-7 PMID: 1730491
  5. Constitutive sensory transduction mutations in the Bordetella pertussis bvgS gene.
    J Bacteriol. 1992 Feb;174(3):970-9 PMID: 1732230
  6. The bvgAS locus negatively controls motility and synthesis of flagella in Bordetella bronchiseptica.
    J Bacteriol. 1992 Feb;174(3):980-90 PMID: 1370665
  7. Bordetella bronchiseptica pneumonia in a patient with Down syndrome: a case report and review.
    Pediatrics. 1992 Jun;89(6 Pt 2):1262-5 PMID: 1534404
  8. Bordetella and Mycoplasma respiratory infections in dogs and cats.
    Vet Clin North Am Small Anim Pract. 1992 Sep;22(5):1173-86 PMID: 1523788
  9. Isolation and characterization of Bordetella bronchiseptica mutants deficient in siderophore activity.
    J Bacteriol. 1993 Feb;175(4):1144-52 PMID: 8381782
  10. A siderophore production mutant of Bordetella bronchiseptica cannot use lactoferrin as an iron source.
    Infect Immun. 1993 Jun;61(6):2698-702 PMID: 8500910
  11. Flagellin gene transcription in Bordetella bronchiseptica is regulated by the BvgAS virulence control system.
    J Bacteriol. 1993 Jun;175(11):3468-79 PMID: 8501051
  12. Pertussis toxin export requires accessory genes located downstream from the pertussis toxin operon.
    Mol Microbiol. 1993 May;8(3):429-34 PMID: 8326857
  13. Nucleotide sequences of the 23S rRNA genes from Bordetella pertussis, B.parapertussis, B.bronchiseptica and B.avium, and their implications for phylogenetic analysis.
    Nucleic Acids Res. 1993 Jul 11;21(14):3320 PMID: 7688118
  14. Chromosomal DNA from both flagellate and non-flagellate Bordetella species contains sequences homologous to the Salmonella H1 flagellin gene.
    FEMS Microbiol Lett. 1993 Aug 1;111(2-3):225-31 PMID: 8405931
  15. Immunogens of Pasteurella.
    Vet Microbiol. 1993 Nov;37(3-4):353-68 PMID: 8116191
  16. BvgAS-mediated signal transduction: analysis of phase-locked regulatory mutants of Bordetella bronchiseptica in a rabbit model.
    Infect Immun. 1994 Aug;62(8):3381-90 PMID: 8039908
  17. Bordetella bronchiseptica pneumonia in a patient with AIDS.
    Thorax. 1994 Jul;49(7):719-20 PMID: 8066571
  18. Microbial iron transport.
    Annu Rev Microbiol. 1994;48:743-72 PMID: 7826025
  19. Identification of alcaligin as the siderophore produced by Bordetella pertussis and B. bronchiseptica.
    J Bacteriol. 1995 Feb;177(4):1116-8 PMID: 7860593
  20. Ectopic expression of the flagellar regulon alters development of the Bordetella-host interaction.
    Cell. 1995 Feb 24;80(4):611-20 PMID: 7867068
  21. Studies on infectious atrophic rhinitis. V. Concept that several agents may cause turbinate atrophy.
    Am J Vet Res. 1956 Jul;17(64):478-84 PMID: 13340110
  22. Autophosphorylation and phosphotransfer in the Bordetella pertussis BvgAS signal transduction cascade.
    Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):1163-7 PMID: 8302847
  23. Nucleotide sequence homology to pertussis toxin gene in Bordetella bronchiseptica and Bordetella parapertussis.
    Infect Immun. 1987 Mar;55(3):497-501 PMID: 3818081
  24. Universal chemical assay for the detection and determination of siderophores.
    Anal Biochem. 1987 Jan;160(1):47-56 PMID: 2952030
  25. Clonal diversity and host distribution in Bordetella bronchiseptica.
    J Bacteriol. 1987 Jun;169(6):2793-803 PMID: 3584070
  26. Bordetella parapertussis and Bordetella bronchiseptica contain transcriptionally silent pertussis toxin genes.
    J Bacteriol. 1987 Jun;169(6):2847-53 PMID: 3584073
  27. The calmodulin-sensitive adenylate cyclase of Bordetella pertussis: cloning and expression in Escherichia coli.
    Mol Microbiol. 1988 Jan;2(1):19-30 PMID: 2897067
  28. Filamentous hemagglutinin of Bordetella pertussis: nucleotide sequence and crucial role in adherence.
    Proc Natl Acad Sci U S A. 1989 Apr;86(8):2637-41 PMID: 2539596
  29. Genetics of pertussis toxin.
    Mol Microbiol. 1989 Jan;3(1):119-24 PMID: 2654538
  30. Use of the promoter fusion transposon Tn5 lac to identify mutations in Bordetella pertussis vir-regulated genes.
    Infect Immun. 1989 Sep;57(9):2674-82 PMID: 2569447
  31. Interactions between Bordetella bronchiseptica and toxigenic Pasteurella multocida in atrophic rhinitis of pigs.
    Res Vet Sci. 1989 Jul;47(1):48-53 PMID: 2772406
  32. Infectious porcine atrophic rhinitis: a review.
    Can Vet J. 1966 Nov;7(11):243-51 PMID: 5342450
  33. A simple chemically defined medium for the production of phase I Bordetella pertussis.
    J Gen Microbiol. 1970 Oct;63(2):211-20 PMID: 4324651
  34. Bordetella bronchiseptica: a re-assessment of its role in canine respiratory disease.
    Vet Rec. 1973 Nov 3;93(18):486-7 PMID: 4594840
  35. Experimental respiratory disease in dogs due to Bordetella bronchiseptica.
    Res Vet Sci. 1976 Jan;20(1):16-23 PMID: 1257623
  36. Iron and infection.
    Microbiol Rev. 1978 Mar;42(1):45-66 PMID: 379572
  37. Biology of Bordetella bronchiseptica.
    Microbiol Rev. 1980 Dec;44(4):722-38 PMID: 7010115
  38. Microbial iron compounds.
    Annu Rev Biochem. 1981;50:715-31 PMID: 6455965
  39. Production and properties of Bordetella pertussis heat-labile toxin.
    J Med Microbiol. 1984 Feb;17(1):91-103 PMID: 6319705
  40. Iron, siderophores, and the pursuit of virulence: independence of the aerobactin and enterochelin iron uptake systems in Escherichia coli.
    Infect Immun. 1986 Mar;51(3):942-7 PMID: 2936686
  41. Genetic diversity and relationships in populations of Bordetella spp.
    J Bacteriol. 1986 Apr;166(1):230-7 PMID: 3957867
  42. Growth and siderophore production by Bordetella pertussis under iron-restricted conditions.
    FEMS Microbiol Lett. 1990 Jan 1;54(1-3):101-5 PMID: 2138989
  43. Autogenous regulation of the Bordetella pertussis bvgABC operon.
    Proc Natl Acad Sci U S A. 1990 May;87(10):3763-7 PMID: 2111016
  44. Adherence of Bordetella bronchiseptica and Pasteurella multocida to swine nasal ciliated epithelial cells in vitro.
    APMIS. 1990 May;98(5):453-61 PMID: 2357345
  45. Fimbrial phase variation in Bordetella pertussis: a novel mechanism for transcriptional regulation.
    EMBO J. 1990 Sep;9(9):2803-9 PMID: 1975238
  46. Positive transcriptional feedback at the bvg locus controls expression of virulence factors in Bordetella pertussis.
    Proc Natl Acad Sci U S A. 1990 Sep;87(17):6753-7 PMID: 1697687
  47. Cellular iron metabolism in health and disease.
    Drug Metab Rev. 1990;22(5):531-79 PMID: 2078994
  48. Human infections associated with Bordetella bronchiseptica.
    Clin Microbiol Rev. 1991 Jul;4(3):243-55 PMID: 1889042
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.177.21.6058-6063.1995
Published
1995-11-00
Pages
6058-63
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC177442
Subset
IM
Grants
PHS HHS · A131548 · United States
External Links
PubMed source DOI: 10.1128/jb.177.21.6058-6063.1995 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2