Home LiteratureArticle Details
PMID: 7559767 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Heparin is an adhesive ligand for the leukocyte integrin Mac-1 (CD11b/CD1).

The Journal of cell biology ·Vol. 130 ·No. 6 ·1995-09-00 ·Pages 1473-82

Diamond MS, Alon R, Parkos CA, Quinn MT, Springer TA

Abstract

Previous studies have demonstrated that the leukocyte integrin Mac-1 adheres to several cell surface and soluble ligands including intercellular adhesion molecule-1, fibrinogen, iC3b, and factor X. However, experiments with Mac-1-expressing transfectants, purified Mac-1, and mAbs to Mac-1 indicate the existence of additional ligands. In this paper, we demonstrate a direct interaction between Mac-1 and heparan sulfate glycans. Heparin affinity resins immunoprecipitate Mac-1, and neutrophils and transfectant cells that express Mac-1 bind to heparin and heparan sulfate, but not to other sulfated glycosaminoglycans. Inhibition studies with mAbs and chemically modified forms of heparin suggest the I domain as a recognition site on Mac-1 for heparin, and suggest that either N- or O-sulfation is sufficient for heparin to bind efficiently to Mac-1. Under conditions of continuous flow in which heparins and E-selectin are cosubstrates, neutrophils tether to E-selectin and form firm adhesions through a Mac-1-heparin interaction.

MeSH Terms
Animals CHO Cells Cell Adhesion Cricetinae Heparin/metabolism Humans Ligands Macrophage-1 Antigen/metabolism Middle Aged Neutrophils/metabolism Sensitivity and Specificity
Chemicals
Ligands Macrophage-1 Antigen Heparin
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Diamond M S
Center for Blood Research, Harvard Medical School, Boston, Massachusetts 02115, USA.
Alon R
Parkos C A
Quinn M T
Springer T A
References (69)
69 references, click to expand
  1. Differential ligand binding specificities of recombinant CD11b/CD18 integrin I-domain.
    J Biol Chem. 1994 Jun 24;269(25):17075-9 PMID: 7516332
  2. Cell surface syndecan-1 on distinct cell types differs in fine structure and ligand binding of its heparan sulfate chains.
    J Biol Chem. 1994 Jul 22;269(29):18881-90 PMID: 8034644
  3. Mac-1 (CD11b/CD18) and CD45 mediate the adhesion of hematopoietic progenitor cells to stromal cell elements via recognition of stromal heparan sulfate.
    Blood. 1994 Aug 1;84(3):739-52 PMID: 8043862
  4. Identification of the complement iC3b binding site in the beta 2 integrin CR3 (CD11b/CD18).
    Proc Natl Acad Sci U S A. 1994 Oct 25;91(22):10680-4 PMID: 7524101
  5. Syndecans, cell surface heparan sulfate proteoglycans, are induced by a proline-rich antimicrobial peptide from wounds.
    Proc Natl Acad Sci U S A. 1994 Nov 8;91(23):11035-9 PMID: 7972004
  6. Perlecan, basal lamina proteoglycan, promotes basic fibroblast growth factor-receptor binding, mitogenesis, and angiogenesis.
    Cell. 1994 Dec 16;79(6):1005-13 PMID: 7528102
  7. Members of the syndecan family of heparan sulfate proteoglycans are expressed in distinct cell-, tissue-, and development-specific patterns.
    Mol Biol Cell. 1994 Jul;5(7):797-805 PMID: 7812048
  8. Drosophila integrins and their ligands.
    Curr Opin Cell Biol. 1994 Oct;6(5):734-9 PMID: 7833052
  9. Rolling of lymphocytes and neutrophils on peripheral node addressin and subsequent arrest on ICAM-1 in shear flow.
    Eur J Immunol. 1995 Apr;25(4):1025-31 PMID: 7537667
  10. The dynamic regulation of integrin adhesiveness.
    Curr Biol. 1994 Jun 1;4(6):506-17 PMID: 7922371
  11. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 1978 May;14(1):9-20 PMID: 667938
  12. Glycosaminoglycans in human neutrophils and leukemic myeloblasts: ultrastructural, cytochemical, immunologic, and biochemical characterization.
    Blood. 1983 Feb;61(2):257-66 PMID: 6401432
  13. Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.
    J Exp Med. 1982 Oct 1;156(4):1000-9 PMID: 7153706
  14. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93 PMID: 6984191
  15. Inhibition of phagocytosis of complement C3- or immunoglobulin G-coated particles and of C3bi binding by monoclonal antibodies to a monocyte-granulocyte membrane glycoprotein (Mol).
    J Clin Invest. 1983 Jul;72(1):171-9 PMID: 6874946
  16. Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies.
    Proc Natl Acad Sci U S A. 1983 Sep;80(18):5699-703 PMID: 6225125
  17. The distribution of the CR3 receptor on human cells and tissue as revealed by a monoclonal antibody.
    Clin Immunol Immunopathol. 1984 Jun;31(3):371-89 PMID: 6232029
  18. Membrane complement receptor type three (CR3) has lectin-like properties analogous to bovine conglutinin as functions as a receptor for zymosan and rabbit erythrocytes as well as a receptor for iC3b.
    J Immunol. 1985 May;134(5):3307-15 PMID: 2984286
  19. Identification of a cell-surface glycoprotein mediating adhesion in human granulocytes.
    Scand J Immunol. 1985 Dec;22(6):619-31 PMID: 2418494
  20. Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.
    J Immunol. 1986 Jul 1;137(1):15-27 PMID: 3519773
  21. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    J Immunol. 1986 Nov 1;137(9):2891-900 PMID: 2428876
  22. Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins.
    Annu Rev Med. 1987;38:175-94 PMID: 3555290
  23. Co-sedimentation of chondroitin sulfate A glycosaminoglycans and proteoglycans with the cytolytic secretory granules of rat large granular lymphocyte (LGL) tumor cells, and identification of a mRNA in normal and transformed LGL that encodes proteoglycans.
    J Immunol. 1987 Aug 1;139(3):863-8 PMID: 3110286
  24. Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface.
    J Clin Invest. 1987 Aug;80(2):535-44 PMID: 3038962
  25. Specificity of membrane complement receptor type three (CR3) for beta-glucans.
    Complement. 1987;4(2):61-74 PMID: 3040332
  26. Purified cytochrome b from human granulocyte plasma membrane is comprised of two polypeptides with relative molecular weights of 91,000 and 22,000.
    J Clin Invest. 1987 Sep;80(3):732-42 PMID: 3305576
  27. The saturable high affinity association of factor X to ADP-stimulated monocytes defines a novel function of the Mac-1 receptor.
    J Biol Chem. 1988 May 25;263(15):7007-15 PMID: 2835359
  28. Neutrophil and monocyte cell surface p150,95 has iC3b-receptor (CR4) activity resembling CR3.
    J Clin Invest. 1988 Aug;82(2):640-51 PMID: 2969921
  29. Complement receptor type three (CD11b/CD18) of human polymorphonuclear leukocytes recognizes fibrinogen.
    Proc Natl Acad Sci U S A. 1988 Oct;85(20):7734-8 PMID: 2971974
  30. Oligospecificity of the cellular adhesion receptor Mac-1 encompasses an inducible recognition specificity for fibrinogen.
    J Cell Biol. 1988 Nov;107(5):1893-900 PMID: 3053736
  31. Structure and biology of proteoglycans.
    Annu Rev Cell Biol. 1988;4:229-55 PMID: 3143379
  32. CR3 (CD11b/CD18) expresses one binding site for Arg-Gly-Asp-containing peptides and a second site for bacterial lipopolysaccharide.
    J Exp Med. 1989 Jan 1;169(1):175-83 PMID: 2462607
  33. Characterization of the major heparan sulfate proteoglycan secreted by bovine aortic endothelial cells in culture. Homology to the large molecular weight molecule of basement membranes.
    J Biol Chem. 1989 Feb 25;264(6):3514-23 PMID: 2521637
  34. The cell surface glycoprotein Mac-1 (CD11b/CD18) mediates neutrophil adhesion and modulates degranulation independently of its quantitative cell surface expression.
    J Immunol. 1989 May 15;142(10):3537-45 PMID: 2565926
  35. Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro.
    J Clin Invest. 1989 Jun;83(6):2008-17 PMID: 2566624
  36. Neutrophil Mac-1 and MEL-14 adhesion proteins inversely regulated by chemotactic factors.
    Science. 1989 Sep 15;245(4923):1238-41 PMID: 2551036
  37. The leukocyte integrins.
    Adv Immunol. 1989;46:149-82 PMID: 2551146
  38. Two leukocyte receptors (CD11a/CD18 and CD11b/CD18) mediate transient adhesion to endothelium by binding to different ligands.
    J Immunol. 1989 Nov 15;143(10):3325-9 PMID: 2809204
  39. Identification of a human peripheral lymph node homing receptor: a rapidly down-regulated adhesion molecule.
    Proc Natl Acad Sci U S A. 1990 Mar;87(6):2244-8 PMID: 2179952
  40. Sequence of human syndecan indicates a novel gene family of integral membrane proteoglycans.
    J Biol Chem. 1990 Apr 25;265(12):6884-9 PMID: 2324102
  41. Neutrophil adherence to isolated adult canine myocytes. Evidence for a CD18-dependent mechanism.
    J Clin Invest. 1990 May;85(5):1497-506 PMID: 1970581
  42. Adhesion receptors of the immune system.
    Nature. 1990 Aug 2;346(6283):425-34 PMID: 1974032
  43. ICAM-1 (CD54): a counter-receptor for Mac-1 (CD11b/CD18).
    J Cell Biol. 1990 Dec;111(6 Pt 2):3129-39 PMID: 1980124
  44. Molecular cloning of a phosphatidylinositol-anchored membrane heparan sulfate proteoglycan from human lung fibroblasts.
    J Cell Biol. 1990 Dec;111(6 Pt 2):3165-76 PMID: 2148568
  45. Leukocyte integrin P150,95 (CD11c/CD18) functions as an adhesion molecule binding to a counter-receptor on stimulated endothelium.
    J Immunol. 1991 Jan 15;146(2):648-55 PMID: 1702811
  46. CD11c/CD18 on neutrophils recognizes a domain at the N terminus of the A alpha chain of fibrinogen.
    Proc Natl Acad Sci U S A. 1991 Feb 1;88(3):1044-8 PMID: 1671533
  47. Leukocytes roll on a selectin at physiologic flow rates: distinction from and prerequisite for adhesion through integrins.
    Cell. 1991 May 31;65(5):859-73 PMID: 1710173
  48. Binding of the integrin Mac-1 (CD11b/CD18) to the third immunoglobulin-like domain of ICAM-1 (CD54) and its regulation by glycosylation.
    Cell. 1991 Jun 14;65(6):961-71 PMID: 1675157
  49. Characterization of ICAM-2 and evidence for a third counter-receptor for LFA-1.
    J Exp Med. 1991 Jul 1;174(1):253-67 PMID: 1676048
  50. Neutrophil migration across a cultured intestinal epithelium. Dependence on a CD11b/CD18-mediated event and enhanced efficiency in physiological direction.
    J Clin Invest. 1991 Nov;88(5):1605-12 PMID: 1682344
  51. The complete sequence of perlecan, a basement membrane heparan sulfate proteoglycan, reveals extensive similarity with laminin A chain, low density lipoprotein-receptor, and the neural cell adhesion molecule.
    J Biol Chem. 1991 Dec 5;266(34):22939-47 PMID: 1744087
  52. Regulated expression and function of CD11c/CD18 integrin on human B lymphocytes. Relation between attachment to fibrinogen and triggering of proliferation through CD11c/CD18.
    J Exp Med. 1991 Dec 1;174(6):1313-22 PMID: 1683891
  53. Leukocyte-endothelial cell recognition: three (or more) steps to specificity and diversity.
    Cell. 1991 Dec 20;67(6):1033-6 PMID: 1760836
  54. Integrins: versatility, modulation, and signaling in cell adhesion.
    Cell. 1992 Apr 3;69(1):11-25 PMID: 1555235
  55. Cell surface heparan sulfate proteoglycans from human vascular endothelial cells. Core protein characterization and antithrombin III binding properties.
    J Biol Chem. 1992 Oct 5;267(28):20435-43 PMID: 1400362
  56. Endothelial cells interact with the core protein of basement membrane perlecan through beta 1 and beta 3 integrins: an adhesion modulated by glycosaminoglycan.
    J Cell Biol. 1992 Nov;119(4):945-59 PMID: 1385448
  57. Expression of the serglycin gene in human leukemic cell lines.
    Leukemia. 1992 Nov;6(11):1143-7 PMID: 1434796
  58. Selectins: interpreters of cell-specific carbohydrate information during inflammation.
    Science. 1992 Nov 6;258(5084):964-9 PMID: 1439808
  59. Biology of the syndecans: a family of transmembrane heparan sulfate proteoglycans.
    Annu Rev Cell Biol. 1992;8:365-93 PMID: 1335744
  60. A subpopulation of Mac-1 (CD11b/CD18) molecules mediates neutrophil adhesion to ICAM-1 and fibrinogen.
    J Cell Biol. 1993 Jan;120(2):545-56 PMID: 7678422
  61. The I domain is a major recognition site on the leukocyte integrin Mac-1 (CD11b/CD18) for four distinct adhesion ligands.
    J Cell Biol. 1993 Feb;120(4):1031-43 PMID: 7679388
  62. A novel divalent cation-binding site in the A domain of the beta 2 integrin CR3 (CD11b/CD18) is essential for ligand binding.
    Cell. 1993 Mar 26;72(6):857-67 PMID: 8458080
  63. Cloning and expression of intercellular adhesion molecule 3 reveals strong homology to other immunoglobulin family counter-receptors for lymphocyte function-associated antigen 1.
    J Exp Med. 1993 Apr 1;177(4):1187-92 PMID: 8459213
  64. Binding to heparan sulfate or heparin enhances neutrophil responses to interleukin 8.
    Proc Natl Acad Sci U S A. 1993 Aug 1;90(15):7158-62 PMID: 8346230
  65. Heparin oligosaccharides bind L- and P-selectin and inhibit acute inflammation.
    Blood. 1993 Dec 1;82(11):3253-8 PMID: 7694675
  66. Neutrophils roll on E-selectin.
    J Immunol. 1993 Dec 1;151(11):6338-46 PMID: 7504018
  67. Traffic signals for lymphocyte recirculation and leukocyte emigration: the multistep paradigm.
    Cell. 1994 Jan 28;76(2):301-14 PMID: 7507411
  68. Drosophila syndecan: conservation of a cell-surface heparan sulfate proteoglycan.
    Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3334-8 PMID: 8159748
  69. The pro-inflammatory seven-transmembrane segment receptors of the leukocyte.
    Curr Opin Immunol. 1994 Feb;6(1):140-5 PMID: 8172674
Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.130.6.1473
Published
1995-09-00
Pages
1473-82
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2120570
Subset
IM
Grants
NIGMS NIH HHS · T32 GM007753 · United States
NIAMS NIH HHS · AR40929 · United States
NCI NIH HHS · CA31799 · United States
NIGMS NIH HHS · T32GM07753-11 · United States
External Links
PubMed source DOI: 10.1083/jcb.130.6.1473 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2