Home LiteratureArticle Details
PMID: 3038962 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Stimulated mobilization of monocyte Mac-1 and p150,95 adhesion proteins from an intracellular vesicular compartment to the cell surface.

The Journal of clinical investigation ·Vol. 80 ·No. 2 ·1987-08-00 ·Pages 535-44

Miller LJ, Bainton DF, Borregaard N, Springer TA

Abstract

Monocytes were stimulated to increase their cell surface quantity of leukocyte adhesion proteins p150,95 and Mac-1 by the chemoattractant formyl-methionyl-leucyl-phenylalanine, or other mediators such as platelet-derived growth factor, tumor necrosis factor, C5a, and leukotriene B4. Dose-response curves indicated variations in the sensitivity of monocytes and granulocytes to these mediators. These increases were independent of protein synthesis and half-maximal at 2 min. Human alveolar and murine peritoneal macrophages, cells that had previously diapedised, could not be induced to upregulate Mac-1 or p150,95. Detergent permeabilization studies in monocytes indicated that these proteins were stored in internal latent pools, which were reduced upon stimulation. Electron microscopy utilizing rabbit antiserum against p150,95 revealed these proteins on the plasma membrane, and in intracellular vesicles and peroxidase negative granules. Together with other functional studies, these findings suggest that the mobilization of Mac-1 and p150,95 from an intracellular compartment to the plasma membrane regulates the monocyte's ability to adhere and diapedese.

MeSH Terms
Animals Antigens, Surface/metabolism Cell Adhesion Cell Adhesion Molecules Cell Compartmentation Cell Membrane/metabolism Complement C5/pharmacology Complement C5a Glycoproteins/pharmacology Granulocytes/metabolism Humans Inflammation/pathology,physiopathology Intracellular Membranes/metabolism Leukotriene B4/pharmacology Macrophage-1 Antigen Macrophages/metabolism Membrane Proteins/metabolism Mice Molecular Weight Monocytes/metabolism N-Formylmethionine Leucyl-Phenylalanine/metabolism Platelet-Derived Growth Factor/pharmacology Receptors, Cell Surface/metabolism Tumor Necrosis Factor-alpha
Chemicals
Antigens, Surface Cell Adhesion Molecules Complement C5 Glycoproteins Macrophage-1 Antigen Membrane Proteins Platelet-Derived Growth Factor Receptors, Cell Surface Tumor Necrosis Factor-alpha Leukotriene B4 N-Formylmethionine Leucyl-Phenylalanine Complement C5a
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Miller L J
Bainton D F
Borregaard N
Springer T A
References (48)
48 references, click to expand
  1. Differentiation of human monocytes in bone marrow and blood. Sequential formation of two granule populations.
    Lab Invest. 1973 Jul;29(1):27-40 PMID: 4728355
  2. Leukocyte adhesion deficiency: an inherited defect in the Mac-1, LFA-1, and p150,95 glycoproteins.
    Annu Rev Med. 1987;38:175-94 PMID: 3555290
  3. Purification of human platelet-derived growth factor.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1809-13 PMID: 287022
  4. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody.
    Eur J Immunol. 1979 Apr;9(4):301-6 PMID: 89034
  5. Chemotaxis of monocytes and neutrophils to platelet-derived growth factor.
    J Clin Invest. 1982 Apr;69(4):1046-9 PMID: 7076844
  6. Antigens on human monocytes identified by monoclonal antibodies.
    J Immunol. 1981 Apr;126(4):1435-42 PMID: 6937561
  7. A novel lymphocyte function-associated antigen (LFA-1): cellular distribution, quantitative expression, and structure.
    J Immunol. 1981 Aug;127(2):596-602 PMID: 6788846
  8. Characterization of an anti-H-2 monoclonal antibody and its use in large-scale antigen purification.
    J Immunol. 1981 Sep;127(3):923-30 PMID: 6167633
  9. Monoclonal antibody to human platelet glycoprotein I. I. Immunological studies.
    Br J Haematol. 1981 Dec;49(4):501-9 PMID: 6459115
  10. Monocyte aggregation and superoxide anion release in response to formyl-methionyl-leucyl-phenylalanine (FMLP) and platelet-activating factor (PAF).
    J Immunol. 1982 May;128(5):1939-44 PMID: 6278021
  11. Complement receptor (CR1) deficiency in erythrocytes from patients with systemic lupus erythematosus.
    J Exp Med. 1982 May 1;155(5):1427-38 PMID: 6978375
  12. Studies on the interaction between GP-18-0-deficient neutrophils and vascular endothelium.
    Blood. 1982 Jul;60(1):160-5 PMID: 7044449
  13. Human neutrophil Fc gamma receptor distribution and structure.
    Proc Natl Acad Sci U S A. 1982 May;79(10):3275-9 PMID: 6808506
  14. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies.
    J Immunol. 1983 Jan;130(1):145-52 PMID: 6183346
  15. Increased expression of C3b receptors on polymorphonuclear leukocytes induced by chemotactic factors and by purification procedures.
    J Immunol. 1983 Jan;130(1):370-5 PMID: 6847888
  16. Severe recurrent bacterial infections associated with defective adherence and chemotaxis in two patients with neutrophils deficient in a cell-associated glycoprotein.
    J Pediatr. 1982 Dec;101(6):932-40 PMID: 7143170
  17. Three distinct antigens associated with human T-lymphocyte-mediated cytolysis: LFA-1, LFA-2, and LFA-3.
    Proc Natl Acad Sci U S A. 1982 Dec;79(23):7489-93 PMID: 6984191
  18. Subcellular localization of the b-cytochrome component of the human neutrophil microbicidal oxidase: translocation during activation.
    J Cell Biol. 1983 Jul;97(1):52-61 PMID: 6408102
  19. Definition of a common leukocyte cell-surface antigen (Lp95-150) associated with diverse cell-mediated immune functions.
    J Immunol. 1983 Dec;131(6):2913-8 PMID: 6644024
  20. A human leukocyte differentiation antigen family with distinct alpha-subunits and a common beta-subunit: the lymphocyte function-associated antigen (LFA-1), the C3bi complement receptor (OKM1/Mac-1), and the p150,95 molecule.
    J Exp Med. 1983 Dec 1;158(6):1785-1803 PMID: 6196430
  21. Redistribution of alpha-granules and their contents in thrombin-stimulated platelets.
    J Cell Biol. 1984 Feb;98(2):748-60 PMID: 6229546
  22. Both Fc receptors and lymphocyte-function-associated antigen 1 on human T gamma lymphocytes are required for antibody-dependent cellular cytotoxicity (killer cell activity).
    Eur J Immunol. 1984 Jun;14(6):518-23 PMID: 6610556
  23. Abnormalities of polymorphonuclear leukocyte function associated with a heritable deficiency of high molecular weight surface glycoproteins (GP138): common relationship to diminished cell adherence.
    J Clin Invest. 1984 Aug;74(2):536-51 PMID: 6746906
  24. Subcellular localization of the large subunit of Mo1 (Mo1 alpha; formerly gp 110), a surface glycoprotein associated with neutrophil adhesion.
    J Clin Invest. 1984 Oct;74(4):1280-90 PMID: 6480827
  25. Deficiency of a leukocyte surface glycoprotein (LFA-1) in two patients with Mo1 deficiency. Effects of cell activation on Mo1/LFA-1 surface expression in normal and deficient leukocytes.
    J Clin Invest. 1984 Oct;74(4):1291-300 PMID: 6237120
  26. Neutrophil specific granules: a fuse that ignites the inflammatory response.
    Clin Res. 1984 Sep;32(3):320-8 PMID: 6488698
  27. Neutrophil-endothelial cell interactions. Modulation of neutrophil adhesiveness induced by complement fragments C5a and C5a des arg and formyl-methionyl-leucyl-phenylalanine in vitro.
    J Clin Invest. 1984 Nov;74(5):1581-92 PMID: 6501563
  28. Inherited deficiency of the Mac-1, LFA-1, p150,95 glycoprotein family and its molecular basis.
    J Exp Med. 1984 Dec 1;160(6):1901-18 PMID: 6096477
  29. Rapid method for isolation of normal human peripheral blood eosinophils on discontinuous Percoll gradients and comparison with neutrophils.
    Blood. 1985 Feb;65(2):433-40 PMID: 3967087
  30. Leukocyte-endothelial interactions.
    Blood. 1985 Mar;65(3):513-25 PMID: 3918593
  31. The monoclonal antibodies alpha S-HCL 1 (alpha Leu-14) and alpha S-HCL 3 (alpha Leu-M5) allow the diagnosis of hairy cell leukemia.
    Blood. 1985 Apr;65(4):974-83 PMID: 3978236
  32. Abnormal adherence-related functions of neutrophils, monocytes, and Epstein-Barr virus-transformed B cells in a patient with C3bi receptor deficiency.
    Blood. 1985 Jun;65(6):1382-90 PMID: 2986743
  33. Human C5a modulates monocyte Fc and C3 receptor expression.
    J Immunol. 1985 Jul;135(1):465-70 PMID: 3158706
  34. The role of neutrophil membrane glycoprotein 150 (Gp-150) in neutrophil-mediated endothelial cell injury in vitro.
    J Immunol. 1985 Jul;135(1):537-43 PMID: 3889157
  35. Platelet-derived growth factor promotes human peripheral monocyte activation.
    Blood. 1985 Jul;66(1):179-83 PMID: 2988667
  36. Human monocyte adherence to cultured vascular endothelium: monoclonal antibody-defined mechanisms.
    J Immunol. 1985 Oct;135(4):2323-30 PMID: 4031492
  37. The severe and moderate phenotypes of heritable Mac-1, LFA-1 deficiency: their quantitative definition and relation to leukocyte dysfunction and clinical features.
    J Infect Dis. 1985 Oct;152(4):668-89 PMID: 3900232
  38. Chemoattractant receptors on phagocytic cells.
    Annu Rev Immunol. 1984;2:257-81 PMID: 6100474
  39. Interleukin 1 acts on cultured human vascular endothelium to increase the adhesion of polymorphonuclear leukocytes, monocytes, and related leukocyte cell lines.
    J Clin Invest. 1985 Nov;76(5):2003-11 PMID: 3877078
  40. Isolation of complement-fragment-iC3b-binding proteins by affinity chromatography. The identification of p150,95 as an iC3b-binding protein.
    Biochem J. 1985 Oct 1;231(1):233-6 PMID: 4062888
  41. p150,95, the third member of the Mac-1, LFA-1 human leukocyte adhesion glycoprotein family.
    J Immunol. 1986 Jan;136(1):240-5 PMID: 3510003
  42. The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules.
    Eur J Immunol. 1986 Mar;16(3):240-8 PMID: 3456894
  43. Interferon-gamma depresses binding of ligand by C3b and C3bi receptors on cultured human monocytes, an effect reversed by fibronectin.
    J Exp Med. 1986 May 1;163(5):1245-59 PMID: 2939170
  44. An endothelial cell surface factor(s) induced in vitro by lipopolysaccharide, interleukin 1, and tumor necrosis factor-alpha increases neutrophil adherence by a CDw18-dependent mechanism.
    J Immunol. 1986 Jun 15;136(12):4548-53 PMID: 3486903
  45. Contributions of the Mac-1 glycoprotein family to adherence-dependent granulocyte functions: structure-function assessments employing subunit-specific monoclonal antibodies.
    J Immunol. 1986 Jul 1;137(1):15-27 PMID: 3519773
  46. The importance of the Mac-1, LFA-1 glycoprotein family in monocyte and granulocyte adherence, chemotaxis, and migration into inflammatory sites: insights from an experiment of nature.
    Ciba Found Symp. 1986;118:102-26 PMID: 3525036
  47. Regulated expression of the Mac-1, LFA-1, p150,95 glycoprotein family during leukocyte differentiation.
    J Immunol. 1986 Nov 1;137(9):2891-900 PMID: 2428876
  48. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens-new tools for genetic analysis.
    Cell. 1978 May;14(1):9-20 PMID: 667938
Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI113102
Published
1987-08-00
Pages
535-44
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC442267
Subset
IM
Grants
NIADDK NIH HHS · AM-10486 · United States
NCI NIH HHS · CA-31799 · United States
External Links
PubMed source DOI: 10.1172/JCI113102 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2