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PMID: 2566624 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Cooperative interactions of LFA-1 and Mac-1 with intercellular adhesion molecule-1 in facilitating adherence and transendothelial migration of human neutrophils in vitro.

The Journal of clinical investigation ·Vol. 83 ·No. 6 ·1989-06-00 ·Pages 2008-17

Smith CW, Marlin SD, Rothlein R, Toman C, Anderson DC

Abstract

The adherence of human neutrophils to human umbilical vein endothelial cells (HUVEC) is partially dependent on the CD11/CD18 family of glycoproteins on the neutrophil and ICAM-1 on the HUVEC. The CD18 heterodimer involved in this adherence was evaluated in vitro using subunit-specific monoclonal antibodies (MAbs). The adherence of unstimulated neutrophils to IL-1-stimulated HUVEC was significantly inhibited by anti-CD11a but not CD11b MAbs, while the adherence of fMLP-stimulated neutrophils was significantly inhibited by both anti-CD11a and -CD11b. Anti-CD11a, but not anti-CD11b MAbs, reduced the adherence of unstimulated neutrophils on purified ICAM-1 to the same low level untreated neutrophils exhibited on a control protein, glycophorin. Stimulation with fMLP significantly increased neutrophil attachment to purified ICAM-1, but not to the control protein. Anti-CD11b MAbs reduced this chemotactically augmented adherence to that of unstimulated neutrophils, and in combination with anti-CD11a MAbs reduced adherence to that on the control protein. The results in this report indicate that unstimulated neutrophils exhibit LFA-1-dependent attachment to ICAM-1, and chemotactic stimulation enhances the attachment of human neutrophils to ICAM-1 by a Mac-1-dependent process.

MeSH Terms
Adult Antigens, Differentiation/immunology Antigens, Surface/immunology,isolation & purification CD18 Antigens Cell Adhesion Cell Adhesion Molecules Cell Communication Cell Movement Endothelium, Vascular/immunology,physiology Humans Lymphocyte Function-Associated Antigen-1 Macrophage-1 Antigen Membrane Glycoproteins/immunology,physiology N-Formylmethionine Leucyl-Phenylalanine/pharmacology Neutrophils/immunology,physiology
Chemicals
Antigens, Differentiation Antigens, Surface CD18 Antigens Cell Adhesion Molecules Lymphocyte Function-Associated Antigen-1 Macrophage-1 Antigen Membrane Glycoproteins N-Formylmethionine Leucyl-Phenylalanine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Smith C W
Speros P. Martel Laboratory of Leukocyte Biology, Baylor College of Medicine, Houston, Texas 77054.
Marlin S D
Rothlein R
Toman C
Anderson D C
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114111
Published
1989-06-00
Pages
2008-17
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC303925
Subset
IM
Grants
NIAID NIH HHS · AI-19031 · United States
NIAID NIH HHS · AI-23521 · United States
NCRR NIH HHS · RR-05425-25 · United States
External Links
PubMed source DOI: 10.1172/JCI114111 PMC Full Text View journal details
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