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PMID: 7556097 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Molecular characterization of the spliceosomal proteins U1A and U2B" from higher plants.

The EMBO journal ·Vol. 14 ·No. 18 ·1995-09-15 ·Pages 4540-50

Simpson GG, Clark GP, Rothnie HM, Boelens W, van Venrooij W, Brown JW

Abstract

In addition to their role in pre-mRNA splicing, the human spliceosomal proteins U1A and U2B" are important models of how RNP motif-containing proteins execute sequence-specific RNA binding. Genes encoding U1A and U2B" have been isolated from potato and thereby provide the only evolutionary comparison available for both proteins and represent the only full-length genes encoding plant spliceosomal proteins to have been cloned and characterized. In vitro RNA binding experiments revealed the ability of potato U2B" to interact with human U2A' to enhance sequence-specific binding and to distinguish cognate RNAs of either plant or animal origin. A comparison of the sequence of U1A and U2B" proteins indicated that multiple residues which could affect RNP motif conformation probably govern the specific distinction in RNA binding by these proteins. Since human U1A modulates polyadenylation in vertebrates, the possibility that plant U1A might be exploited in the characterization of this process in plants was examined. However, unlike vertebrate U1A, neither U1A from potato nor Arabidopsis bound their own mRNA and no evidence for binding to upstream efficiency elements in polyadenylation signals was obtained, suggesting that plant U1A is not involved in polyadenylation.

MeSH Terms
Amino Acid Sequence Autoantigens Base Sequence Genomic Library Humans Molecular Sequence Data Protein Binding Protein Biosynthesis RNA Precursors/metabolism RNA Processing, Post-Transcriptional RNA, Small Nuclear/metabolism RNA-Binding Proteins Ribonucleoprotein, U1 Small Nuclear/genetics,metabolism Ribonucleoprotein, U2 Small Nuclear/genetics,metabolism Ribonucleoproteins, Small Nuclear Sequence Analysis, DNA Sequence Homology, Amino Acid Solanum tuberosum/genetics Species Specificity Spliceosomes Transcription, Genetic snRNP Core Proteins
Chemicals
Autoantigens RNA Precursors RNA, Small Nuclear RNA-Binding Proteins Ribonucleoprotein, U1 Small Nuclear Ribonucleoprotein, U2 Small Nuclear Ribonucleoproteins, Small Nuclear SNRPB2 protein, human U1A protein U2A' protein, human snRNP Core Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Simpson G G
Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK.
Clark G P
Rothnie H M
Boelens W
van Venrooij W
Brown J W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1995-09-15
Pages
4540-50
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394546
Subset
IM
Databases
GENBANK
Z49990, Z49991
External Links
PubMed source PMC Full Text View journal details
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