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PMID: 7525274 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Decay-accelerating factor CD55 is identified as the receptor for echovirus 7 using CELICS, a rapid immuno-focal cloning method.

The EMBO journal ·Vol. 13 ·No. 21 ·1994-11-01 ·Pages 5070-4

Ward T, Pipkin PA, Clarkson NA, Stone DM, Minor PD, Almond JW

Abstract

Using an anti-receptor mAb that blocks the attachment of echovirus 7 and related viruses (echoviruses 13, 21, 29 and 33), we have isolated a complementary DNA clone that encodes the human decay-accelerating factor (CD55). Mouse cells transfected with the CD55 clone bind echovirus 7, and this binding is blocked by the anti-receptor mAb. The method used (CELICS) allows rapid and direct cloning of genes encoding cell surface receptors. It is based on episomal replication and high efficiency expression of complementary DNA clones in the vector pCDM8 in COS or WOP cells, in conjunction with a sensitive immuno-focal screen that uses antibody probes linked to beta-galactosidase. Receptor positive cells were identified by a colour change and isolated individually using a micromanipulator. DNA extracted from a small number of cells was then cloned directly in Escherichia coli.

MeSH Terms
Animals Antigens, CD/genetics CD55 Antigens Cells, Cultured Cloning, Molecular/methods Enterovirus B, Human Humans Intercellular Adhesion Molecule-1/genetics Membrane Glycoproteins/genetics Receptors, Virus/genetics Selection, Genetic Transfection
Chemicals
Antigens, CD CD55 Antigens Membrane Glycoproteins Receptors, Virus Intercellular Adhesion Molecule-1
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Ward T
School of Animal and Microbial Sciences, University of Reading, Whiteknights, UK.
Pipkin P A
Clarkson N A
Stone D M
Minor P D
Almond J W
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-11-01
Pages
5070-4
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395453
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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