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PMID: 1583722 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human immunodeficiency virus type 2 infection and fusion of CD4-negative human cell lines: induction and enhancement by soluble CD4.

Journal of virology ·Vol. 66 ·No. 6 ·1992-06-00 ·Pages 3531-7

Clapham PR, McKnight A, Weiss RA

Abstract

We describe human immunodeficiency type 2 (HIV-2) strains which induce cell-to-cell fusion and infect certain CD4- human cell lines. Soluble CD4 (sCD4) induces or enhances fusion by most HIV-2 strains tested. Soluble CD4-immunoglobulin G chimeras and conjugates of sCD4 and antibody to the third domain of CD4 block HIV-2 fusion of CD4- cells. We conclude that HIV-2 can enter CD4- cells via an alternative cell surface receptor to CD4. While some strains entered efficiently, others retained a dependency on an interaction with sCD4 to initiate changes in the virion envelope required for membrane fusion.

MeSH Terms
Acquired Immunodeficiency Syndrome/microbiology CD4 Antigens/metabolism Cell Fusion/drug effects,physiology Cell Line DNA Mutational Analysis HIV Envelope Protein gp120/metabolism HIV-2/pathogenicity Humans Receptors, HIV/metabolism Recombinant Fusion Proteins/metabolism Rhabdomyosarcoma/microbiology Tumor Cells, Cultured
Chemicals
CD4 Antigens HIV Envelope Protein gp120 Receptors, HIV Recombinant Fusion Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Clapham P R
Chester Beatty Laboratories, Institute of Cancer Research, London, England.
McKnight A
Weiss R A
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34 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.66.6.3531-3537.1992
Published
1992-06-00
Pages
3531-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC241134
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.66.6.3531-3537.1992 PMC Full Text View journal details
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