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PMID: 7523695 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Analysis of the primary T-cell response to Sendai virus infection in C57BL/6 mice: CD4+ T-cell recognition is directed predominantly to the hemagglutinin-neuraminidase glycoprotein.

Journal of virology ·Vol. 68 ·No. 11 ·1994-11-00 ·Pages 6863-70

Cole GA, Katz JM, Hogg TL, Ryan KW, Portner A, Woodland DL

Abstract

Sendai virus infection of C57BL/6 mice elicits a strong CD4+ and CD8+ T-cell response in the respiratory tract. To investigate the specificity of the CD4+ T-cell response, a panel of hybridomas was generated from cells recovered from the respiratory tracts of infected mice. Using vaccinia virus recombinants expressing individual Sendai virus proteins, we found that the majority of these hybridomas (34 of 37) were specific for the hemagglutinin-neuraminidase (HN) glycoprotein. The hybridomas were then analyzed for reactivity to a set of overlapping peptides spanning the entire length of the hemagglutinin-neuraminidase glycoprotein. At least five H-2 I-Ab-restricted epitopes were defined in HN. The strong bias toward recognition of class II epitopes derived from a single viral protein contrasts with T-cell recognition of epitopes of several proteins in influenza A virus as found previously by others.

MeSH Terms
Amino Acid Sequence Animals CD4-Positive T-Lymphocytes/immunology Epitopes Female H-2 Antigens/physiology HN Protein/immunology Hybridomas/immunology Mice Mice, Inbred C57BL Molecular Sequence Data Parainfluenza Virus 1, Human/immunology Paramyxoviridae Infections/immunology Peptide Fragments/immunology
Chemicals
Epitopes H-2 Antigens HN Protein Peptide Fragments
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Cole G A
Department of Immunology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105.
Katz J M
Hogg T L
Ryan K W
Portner A
Woodland D L
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.68.11.6863-6870.1994
Published
1994-11-00
Pages
6863-70
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237121
Subset
IM
Grants
NIAID NIH HHS · AI 31596 · United States
External Links
PubMed source DOI: 10.1128/JVI.68.11.6863-6870.1994 PMC Full Text View journal details
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