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PMID: 6238333 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Antigen-specific helper T-cell clone supernatant is sufficient to induce both polyclonal proliferation and differentiation of small resting B lymphocytes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 20 ·1984-10-00 ·Pages 6491-5

Leclercq L, Bismuth G, Thèze J

Abstract

Gradient-purified resting B lymphocytes can be polyclonally stimulated by antigen-specific major histocompatibility complex (MHC)-restricted helper T lymphocytes as well as by antigen-activated helper T-cell supernatant. In contrast to what has been described so far, we show that helper T-cell supernatant (in the absence of any other added stimulus, such as that provided by anti-mu antibodies) is sufficient to induce both proliferation of resting B cells and their differentiation into IgM-secreting cells. The stimulation induced by the helper T-cell supernatant takes place in serum-free medium and is not MHC-restricted. Our findings strongly support the existence of a B-cell activating factor acting on the resting B cell and causing it to enter the G1 phase of the cell cycle in a MHC-unrestricted manner.

MeSH Terms

Animals B-Lymphocytes/immunology Cell Differentiation Cells, Cultured Culture Media Lymphocyte Activation Major Histocompatibility Complex Mice Mice, Inbred Strains T-Lymphocytes, Helper-Inducer/immunology

Chemicals

Culture Media

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Leclercq L

Bismuth G

Thèze J

References (23)

23 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.81.20.6491

Published

1984-10-00

Pages

6491-5

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC391950

Subset

External Links

PubMed source DOI: 10.1073/pnas.81.20.6491 PMC Full Text View journal details

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Antigen-specific helper T-cell clone supernatant is sufficient to induce both polyclonal proliferation and differentiation of small resting B lymphocytes.

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