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PMID: 7504058 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Short consensus repeat-3 domain of recombinant decay-accelerating factor is recognized by Escherichia coli recombinant Dr adhesin in a model of a cell-cell interaction.

The Journal of experimental medicine ·Vol. 178 ·No. 6 ·1993-12-01 ·Pages 2115-21

Nowicki B, Hart A, Coyne KE, Lublin DM, Nowicki S

Abstract

A bacterial pathogen that is important in both urinary tract and intestinal infections is Escherichia coli which expresses Dr or related adhesins. In this report, we present a model for testing cell-cell interaction, using both molecularly characterized laboratory cells that express recombinant molecules of human decay-accelerating factor (DAF), and recombinant bacterial Dr colonization factors. Dr adhesin ligand was identified as DAF (CD55), a membrane protein that protects autologous tissues from damage due to the complement system. Structure-function studies mapped the adhesin-binding site on the DAF molecule. A single-point substitution in the third short consensus repeat domain, Ser165 to Leu, corresponding to the Dra to Drb allelic polymorphism, caused complete abolition of adhesin binding to DAF.

MeSH Terms
Adhesins, Escherichia coli Antigens, Bacterial/metabolism Antigens, CD/chemistry,metabolism Bacterial Adhesion Bacterial Outer Membrane Proteins/metabolism Binding Sites CD55 Antigens Cell Adhesion Humans In Vitro Techniques Ligands Membrane Glycoproteins/chemistry,metabolism Protein Binding Recombinant Proteins Structure-Activity Relationship
Chemicals
Adhesins, Escherichia coli Antigens, Bacterial Antigens, CD Bacterial Outer Membrane Proteins CD55 Antigens Ligands Membrane Glycoproteins Recombinant Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nowicki B
Department of Obstetrics and Gynecology, University of Texas Medical Branch, Galveston 77550.
Hart A
Coyne K E
Lublin D M
Nowicki S
References (30)
30 references, click to expand
  1. Inhibition of complement by a substance isolated from human erythrocytes. I. Extraction from human erythrocyte stromata.
    Immunochemistry. 1969 May;6(3):391-403 PMID: 5786933
  2. Mapping of epitopes, glycosylation sites, and complement regulatory domains in human decay accelerating factor.
    J Immunol. 1992 Nov 1;149(9):2906-13 PMID: 1383332
  3. New Method for isolation of immunologically pure pili from Escherichia coli.
    Infect Immun. 1980 Feb;27(2):569-75 PMID: 6103871
  4. Purification from guinea pig erythrocyte stroma of a decay-accelerating factor for the classical c3 convertase, C4b,2a.
    J Immunol. 1981 Nov;127(5):2035-9 PMID: 6913607
  5. Mannose-resistant haemagglutination and P antigen recognition are characteristic of Escherichia coli causing primary pyelonephritis.
    Lancet. 1981 Dec 19-26;2(8260-61):1366-9 PMID: 6171696
  6. Chronic diarrhea in infants caused by adherent enteropathogenic Escherichia coli.
    J Pediatr. 1982 Mar;100(3):358-61 PMID: 7038073
  7. Isolation of a human erythrocyte membrane glycoprotein with decay-accelerating activity for C3 convertases of the complement system.
    J Immunol. 1982 Jul;129(1):184-9 PMID: 6211481
  8. A possible null phenotype in the Cromer blood group complex.
    Transfusion. 1982 Sep-Oct;22(5):362-3 PMID: 7123631
  9. Affected erythrocytes of patients with paroxysmal nocturnal hemoglobinuria are deficient in the complement regulatory protein, decay accelerating factor.
    Proc Natl Acad Sci U S A. 1983 Aug;80(16):5066-70 PMID: 6576376
  10. Deficiency of an erythrocyte membrane protein with complement regulatory activity in paroxysmal nocturnal hemoglobinuria.
    Proc Natl Acad Sci U S A. 1983 Sep;80(17):5430-4 PMID: 6225118
  11. Genetic organization of the afimbrial adhesin operon and nucleotide sequence from a uropathogenic Escherichia coli gene encoding an afimbrial adhesin.
    J Bacteriol. 1985 Jun;162(3):1285-92 PMID: 2860096
  12. Distribution of decay-accelerating factor in the peripheral blood of normal individuals and patients with paroxysmal nocturnal hemoglobinuria.
    J Exp Med. 1985 Jul 1;162(1):75-92 PMID: 2409211
  13. Biosynthesis and glycosylation of the human complement regulatory protein decay-accelerating factor.
    J Immunol. 1986 Sep 1;137(5):1629-35 PMID: 2427577
  14. Expression of P, type-1, and type-1C fimbriae of Escherichia coli in the urine of patients with acute urinary tract infection.
    J Infect Dis. 1987 Oct;156(4):567-74 PMID: 2887621
  15. Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure.
    Proc Natl Acad Sci U S A. 1987 Nov;84(21):7413-7 PMID: 2823261
  16. A human cell-surface glycoprotein that carries Cromer-related blood group antigens on erythrocytes and is also expressed on leucocytes and platelets.
    Immunology. 1987 Oct;62(2):307-13 PMID: 3679286
  17. Molecular cloning of the Escherichia coli O75X adhesin.
    Infect Immun. 1987 Dec;55(12):3168-73 PMID: 2890586
  18. Antiphagocytic activity of streptococcal M protein: selective binding of complement control protein factor H.
    Proc Natl Acad Sci U S A. 1988 Mar;85(5):1657-61 PMID: 2964038
  19. A hemagglutinin of uropathogenic Escherichia coli recognizes the Dr blood group antigen.
    Infect Immun. 1988 May;56(5):1057-60 PMID: 2895740
  20. Uropathogenic Escherichia coli can express serologically identical pili of different receptor binding specificities.
    Mol Microbiol. 1988 Mar;2(2):255-63 PMID: 2898091
  21. Identification of human erythrocyte blood group antigens on decay-accelerating factor (DAF) and an erythrocyte phenotype negative for DAF.
    J Exp Med. 1988 Jun 1;167(6):1993-8 PMID: 2455016
  22. Use of the Dr hemagglutinin of uropathogenic Escherichia coli to differentiate normal from abnormal red cells in paroxysmal nocturnal hemoglobinuria.
    N Engl J Med. 1988 Nov 10;319(19):1289-90 PMID: 3054548
  23. Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells.
    J Bacteriol. 1989 Aug;171(8):4281-9 PMID: 2568985
  24. The Dr hemagglutinin, afimbrial adhesins AFA-I and AFA-III, and F1845 fimbriae of uropathogenic and diarrhea-associated Escherichia coli belong to a family of hemagglutinins with Dr receptor recognition.
    Infect Immun. 1990 Jan;58(1):279-81 PMID: 1967170
  25. Decay-accelerating factor and membrane cofactor protein.
    Curr Top Microbiol Immunol. 1990;153:123-45 PMID: 1688754
  26. pap-2-encoded fimbriae adhere to the P blood group-related glycosphingolipid stage-specific embryonic antigen 4 in the human kidney.
    Infect Immun. 1990 Dec;58(12):4055-62 PMID: 1979319
  27. Molecular structure of the Dr adhesin: nucleotide sequence and mapping of receptor-binding domain by use of fusion constructs.
    Infect Immun. 1991 Jan;59(1):261-8 PMID: 1670929
  28. Dr(a-) polymorphism of decay accelerating factor. Biochemical, functional, and molecular characterization and production of allele-specific transfectants.
    J Clin Invest. 1991 Jun;87(6):1945-52 PMID: 1710232
  29. Phospholipid-anchored and transmembrane versions of either decay-accelerating factor or membrane cofactor protein show equal efficiency in protection from complement-mediated cell damage.
    J Exp Med. 1991 Jul 1;174(1):35-44 PMID: 1711565
  30. Prevention of colonization of the urinary tract of mice with Escherichia coli by blocking of bacterial adherence with methyl alpha-D-mannopyranoside.
    J Infect Dis. 1979 Mar;139(3):329-32 PMID: 376757
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.178.6.2115
Published
1993-12-01
Pages
2115-21
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2191283
Subset
IM
Grants
NIAID NIH HHS · AI-15322 · United States
NIDDK NIH HHS · DK-42029 · United States
External Links
PubMed source DOI: 10.1084/jem.178.6.2115 PMC Full Text View journal details
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