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PMID: 1979319 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

pap-2-encoded fimbriae adhere to the P blood group-related glycosphingolipid stage-specific embryonic antigen 4 in the human kidney.

Infection and immunity ·Vol. 58 ·No. 12 ·1990-12-00 ·Pages 4055-62

Karr JF, Nowicki BJ, Truong LD, Hull RA, Moulds JJ, Hull SI

Abstract

A subtype of P fimbriae, encoded by the pap-2 gene cluster, has been analyzed for agglutination of erythrocytes and for binding to cryostat sections of the human kidney. We have demonstrated that pap-2-encoded fimbriae are capable of binding to erythrocytes from some animal species and to human erythrocytes which express globoside and the LKE (stage-specific embryonic antigen 4 [SSEA-4]) antigen. The pap-2 fimbriae bind to Bowman's capsule in the human kidney. Monoclonal antibodies directed against glycosphingolipids were used for the detection of specific P blood group-related antigens in the human kidney and on erythrocytes. Preincubation of kidney sections with monoclonal antibody MC813-70, which binds to the SSEA-4 antigen, inhibited adherence of purified pap-2-encoded fimbriae to Bowman's capsule. We suggest that one receptor for pap-2-encoded fimbriae is the antigen known as LKE (Luke) on human erythrocytes or SSEA-4 in the tissues.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens/analysis Bacterial Adhesion Carbohydrate Sequence Escherichia coli/genetics,immunology Fimbriae, Bacterial/immunology Glycosphingolipids/immunology Hemagglutination Humans In Vitro Techniques Kidney/microbiology Molecular Sequence Data P Blood-Group System Stage-Specific Embryonic Antigens
Chemicals
Antibodies, Monoclonal Antigens Glycosphingolipids P Blood-Group System Stage-Specific Embryonic Antigens stage-specific embryonic antigen-4
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Karr J F
Department of Microbiology and Immunology, Baylor College of Medicine, Houston, Texas 77030.
Nowicki B J
Truong L D
Hull R A
Moulds J J
Hull S I
References (43)
43 references, click to expand
  1. Frequency of gene sequences necessary for pyelonephritis-associated pili expression among isolates of Enterobacteriaceae from human extraintestinal infections.
    Infect Immun. 1984 Mar;43(3):1064-7 PMID: 6141999
  2. Monoclonal antibodies that bind to galactosylgloboside (SSEA-3 antigen).
    Arch Biochem Biophys. 1988 May 1;262(2):620-5 PMID: 3364985
  3. Localization of glycosphingolipids in human tissues by immunofluorescence.
    J Immunol. 1970 Jun;104(6):1530-9 PMID: 4986771
  4. Introital enterobacteria, urinary infection, and the urethral syndrome.
    Lancet. 1970 Dec 12;2(7685):1208-10 PMID: 4098657
  5. The periurethral aerobic bacterial flora in healthy boys and girls.
    Acta Paediatr Scand. 1976 Jan;65(1):74-80 PMID: 766562
  6. The periurethral aerobic flora in girls highly susceptible to urinary infections.
    Acta Paediatr Scand. 1976 Jan;65(1):81-7 PMID: 766563
  7. Studies of introital colonization in women with recurrent urinary infections. VII. The role of bacterial adherence.
    J Urol. 1977 Apr;117(4):472-6 PMID: 321809
  8. Uropathogenic Escherichia coli can express serologically identical pili of different receptor binding specificities.
    Mol Microbiol. 1988 Mar;2(2):255-63 PMID: 2898091
  9. Presence of the Dr receptor in normal human tissues and its possible role in the pathogenesis of ascending urinary tract infection.
    Am J Pathol. 1988 Oct;133(1):1-4 PMID: 3052090
  10. Molecular analysis and epidemiology of the Dr hemagglutinin of uropathogenic Escherichia coli.
    Infect Immun. 1989 Feb;57(2):446-51 PMID: 2643568
  11. LKE red cell antigen and its relationship to P1 and Pk: serological study of a large family.
    Vox Sang. 1988;55(4):237-40 PMID: 3218169
  12. Role of S- and common-type I-fimbriae of Escherichia coli in experimental upper and lower urinary tract infection.
    Microb Pathog. 1987 Mar;2(3):223-6 PMID: 2907084
  13. Binding specificities of wild-type and cloned Escherichia coli strains that recognize globo-A.
    Infect Immun. 1989 Nov;57(11):3389-94 PMID: 2572551
  14. Purified P fimbriae from two cloned gene clusters of a single pyelonephritogenic strain adhere to unique structures in the human kidney.
    Infect Immun. 1989 Nov;57(11):3594-600 PMID: 2572557
  15. Frequency and organization of pap homologous DNA in relation to clinical origin of uropathogenic Escherichia coli.
    J Infect Dis. 1990 Mar;161(3):518-24 PMID: 1968935
  16. AN AGGLUTININ ASSOCIATED WITH THE P AND THE ABO BLOOD GROUP SYSTEMS.
    Vox Sang. 1965 May-Jun;10:269-80 PMID: 14332927
  17. Isoantigenic expression of Forssman glycolipid in human gastric and colonic mucosa: its possible identity with "A-like antigen" in human cancer.
    Proc Natl Acad Sci U S A. 1977 Jul;74(7):3023-7 PMID: 268649
  18. Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.
    Eur J Immunol. 1978 Aug;8(8):539-51 PMID: 81133
  19. Aerobic and anaerobic urethral flora of healthy females in various physiological age groups and of females with urinary tract infections.
    J Clin Microbiol. 1980 Jun;11(6):654-9 PMID: 7000816
  20. Construction and expression of recombinant plasmids encoding type 1 or D-mannose-resistant pili from a urinary tract infection Escherichia coli isolate.
    Infect Immun. 1981 Sep;33(3):933-8 PMID: 6116675
  21. Occurrence of P-fimbriated Escherichia coli in urinary tract infections.
    Lancet. 1981 Dec 19-26;2(8260-61):1369-72 PMID: 6171697
  22. Distribution of murine stage-specific embryonic antigens in the kidneys of three rodent species.
    Exp Cell Res. 1982 Aug;140(2):331-9 PMID: 6126385
  23. A novel pentaglycosyl ceramide containing di-beta-N-acetylgalactos-aminyl residue (Para-Forssman glycolipid) isolated from human erythrocyte membrane.
    Adv Exp Med Biol. 1982;152:71-81 PMID: 6814203
  24. Monoclonal antibody to murine embryos defines a stage-specific embryonic antigen expressed on mouse embryos and human teratocarcinoma cells.
    Cell. 1982 Oct;30(3):697-705 PMID: 6183004
  25. Contribution of adhesion to bacterial persistence in the mouse urinary tract.
    Infect Immun. 1983 Apr;40(1):265-72 PMID: 6131870
  26. Role of type 1 fimbriae in the pathogenesis of ascending urinary tract infection induced by escherichia coli in mice.
    Infect Immun. 1983 Mar;39(3):1307-15 PMID: 6132876
  27. New globoseries glycosphingolipids in human teratocarcinoma reactive with the monoclonal antibody directed to a developmentally regulated antigen, stage-specific embryonic antigen 3.
    J Biol Chem. 1983 Jul 25;258(14):8934-42 PMID: 6863318
  28. Stage-specific embryonic antigens (SSEA-3 and -4) are epitopes of a unique globo-series ganglioside isolated from human teratocarcinoma cells.
    EMBO J. 1983;2(12):2355-61 PMID: 6141938
  29. Blood group A glycolipid (Ax) with globo-series structure which is specific for blood group A1 erythrocytes: one of the chemical bases for A1 and A2 distinction.
    Biochem Biophys Res Commun. 1984 Oct 30;124(2):523-9 PMID: 6497890
  30. Structural characterization of a blood group A heptaglycosylceramide with globo-series structure. The major glycolipid based blood group A antigen of human kidney.
    FEBS Lett. 1985 Jan 1;179(1):165-72 PMID: 3965299
  31. Serotypes, hemolysin production, and receptor recognition of Escherichia coli strains associated with neonatal sepsis and meningitis.
    Infect Immun. 1985 May;48(2):486-91 PMID: 2580792
  32. Specificity of binding of a strain of uropathogenic Escherichia coli to Gal alpha 1----4Gal-containing glycosphingolipids.
    J Biol Chem. 1985 Jul 15;260(14):8545-51 PMID: 3891756
  33. Immunochemical studies on blood groups. The combining site specificities of mouse monoclonal hybridoma anti-A and anti-B.
    J Biol Chem. 1985 Oct 25;260(24):13208-17 PMID: 2414276
  34. The specific glycosphingolipid composition of human ureteral epithelial cells.
    J Biochem. 1985 Nov;98(5):1169-80 PMID: 4086474
  35. A murine monoclonal IgM antibody specific for blood group P antigen (globoside)
    Br J Haematol. 1986 May;63(1):35-46 PMID: 2423110
  36. Red cell antigens P (globoside) and Luke: identification by monoclonal antibodies defining the murine stage-specific embryonic antigens -3 and -4 (SSEA-3 and SSEA-4).
    Vox Sang. 1986;51(1):53-6 PMID: 2874662
  37. Identification and characterization of a uroepithelial cell adhesin from a uropathogenic isolate of Proteus mirabilis.
    Infect Immun. 1986 Oct;54(1):43-9 PMID: 2875952
  38. Separation of blood group A-active oligosaccharides by high-pressure liquid affinity chromatography using a monoclonal antibody bound to concanavalin A silica.
    Anal Biochem. 1987 Feb 15;161(1):140-3 PMID: 3034096
  39. The PapG protein is the alpha-D-galactopyranosyl-(1----4)-beta-D-galactopyranose-binding adhesin of uropathogenic Escherichia coli.
    Proc Natl Acad Sci U S A. 1987 Aug;84(16):5898-902 PMID: 2886993
  40. Glycosphingolipids of human umbilical vein endothelial cells and smooth muscle cells.
    Arch Biochem Biophys. 1987 Aug 1;256(2):435-45 PMID: 3304165
  41. Nucleotide and translated amino acid sequences of cDNA coding for the variable regions of the light and heavy chains of mouse hybridoma antibodies to blood group A and B substances.
    J Biol Chem. 1987 Oct 5;262(28):13579-83 PMID: 3115981
  42. Escherichia coli urethritis in women with symptoms of acute urinary tract infection.
    J Infect Dis. 1988 Jan;157(1):196-9 PMID: 3275723
  43. The urethra and its relationship to urinary tract infection: the flora of the normal female urethra.
    South Med J. 1966 May;59(5):621-6 PMID: 5326960
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.12.4055-4062.1990
Published
1990-12-00
Pages
4055-62
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC313776
Subset
IM
Grants
NIAID NIH HHS · AI21009 · United States
External Links
PubMed source DOI: 10.1128/iai.58.12.4055-4062.1990 PMC Full Text View journal details
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