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PMID: 2890586 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning of the Escherichia coli O75X adhesin.

Infection and immunity ·Vol. 55 ·No. 12 ·1987-12-00 ·Pages 3168-73

Nowicki B, Barrish JP, Korhonen T, Hull RA, Hull SI

Abstract

The uropathogenic strain Escherichia coli IH11128 (O75:K5:H-) exhibits a mannose-resistant O75X adhesin. The genes encoding the O75X adhesin were cloned from a clinical strain and transferred to E. coli K-12 derivatives. The recombinant plasmids were found to express a 15-kilodalton fimbrial subunit protein, a fimbrialike extracellular structure, and mannose-resistant hemagglutination. An indirect immunofluorescence assay was used to study attachment of the clone and purified adhesin to frozen sections of human kidney. The clone bound selectively to the interstitial areas and notably to Bowman's capsule. The purified adhesin bound to the basement membrane of the tubules and to Bowman's capsule.

MeSH Terms
Adhesins, Escherichia coli Bacterial Adhesion Bacterial Proteins/genetics Basement Membrane/microbiology Cloning, Molecular Escherichia coli/genetics Fimbriae, Bacterial Genes, Bacterial Hemagglutination Hemagglutinins/genetics Kidney/microbiology Molecular Weight Plasmids
Chemicals
Adhesins, Escherichia coli Bacterial Proteins Hemagglutinins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Nowicki B
Department of Microbiology and Immunology, Baylor College of Medicine, Texas Medical Center, Houston.
Barrish J P
Korhonen T
Hull R A
Hull S I
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.55.12.3168-3173.1987
Published
1987-12-00
Pages
3168-73
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260044
Subset
IM
Grants
NIAID NIH HHS · AI18462 · United States
NIAID NIH HHS · AI21009 · United States
External Links
PubMed source DOI: 10.1128/iai.55.12.3168-3173.1987 PMC Full Text View journal details
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