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PMID: 7494336 Published · ppublish English Case Reports Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Complete genetic characterization and analysis of isolation of Sin Nombre virus.

Journal of virology ·Vol. 69 ·No. 12 ·1995-12-00 ·Pages 8132-6

Chizhikov VE, Spiropoulou CF, Morzunov SP, Monroe MC, Peters CJ, Nichol ST

Abstract

This study reports completion of the genetic characterization of the entire genome of Sin Nombre (SN) virus (NMH10) detected in autopsy tissues from a patient who died of hantavirus pulmonary syndrome (HPS). The large (L) genome segment was found to be 6,562 nucleotides in length and encoded a putative L polymerase that was 2,153 amino acids in length. No evidence of segment reassortment with other well-characterized hantaviruses was obtained. The sequence of the entire S, M, and L genome segments of SN virus (strain NMR11) isolated from a mouse (trapped in the residence of the patient infected with SN virus [NMH10]) by passage two times in Peromyscus maniculatus and then by five passages in E6 Vero cells was determined and compared with that of the virus detected in autopsy tissues. Only 16 nucleotide differences were detected between the virus genomes, and none of these resulted in virus protein amino acid substitutions. Determination of the exact 5'- and 3'-terminal sequences of all genome segments of SN virus and representatives of other serologic groups in the Hantavirus genus, family Bunyaviridae, showed the existence of conserved nucleotide domains that may be involved in important regulatory mechanisms, such as RNA encapsidation, polymerase binding, and control of transcription and replication.

MeSH Terms
Amino Acid Sequence Animals Autopsy Base Sequence Bunyaviridae/classification,genetics Chlorocebus aethiops Fatal Outcome Genome, Viral Hantavirus/classification,genetics,isolation & purification Hantavirus Pulmonary Syndrome/virology Humans Mice Molecular Sequence Data Peromyscus Phylogeny Sequence Homology, Nucleic Acid Vero Cells
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Chizhikov V E
Special Pathogens Branch, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30333, USA.
Spiropoulou C F
Morzunov S P
Monroe M C
Peters C J
Nichol S T
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.69.12.8132-8136.1995
Published
1995-12-00
Pages
8132-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC189768
Subset
IM
Grants
NIAID NIH HHS · AI 12680 · United States
Databases
GENBANK
L37901, L37902, L37903, L37904
External Links
PubMed source DOI: 10.1128/JVI.69.12.8132-8136.1995 PMC Full Text View journal details
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