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PMID: 1697911 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Anti-mRNAs in La Crosse bunyavirus-infected cells.

Journal of virology ·Vol. 64 ·No. 10 ·1990-10-00 ·Pages 5051-7

Hacker D, Rochat S, Kolakofsky D

Abstract

Unlike some members of the family Bunyaviridae which contain ambisense genomes, all La Crosse virus reading frames are translated from antigenome sense mRNAs. Nevertheless, La Crosse virus genome sense mRNAs or anti-mRNAs are initiated from antigenome templates. These are characterized by the same range of capped, nontemplated sequences at their 5' ends as mRNAs, but their 3' ends are presumed to be heterogenous, as they were not seen on RNA blots. The anti-mRNAs are estimated to be 15 to 30 times less abundant than mRNAs, but remarkably, this ratio is similar to that of functional genome sense mRNAs made from other bona fide ambisense segments. A role for these anti-mRNAs during infection is unclear.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Bunyaviridae/genetics Cell Line Cell Transformation, Viral Cloning, Molecular Genes, Viral Molecular Sequence Data Molecular Weight Oligonucleotide Probes RNA/genetics RNA, Antisense RNA, Messenger/antagonists & inhibitors,genetics,isolation & purification Sequence Homology, Nucleic Acid
Chemicals
Oligonucleotide Probes RNA, Antisense RNA, Messenger RNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hacker D
Department of Microbiology, University of Geneva School of Medicine, Switzerland.
Rochat S
Kolakofsky D
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35 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.10.5051-5057.1990
Published
1990-10-00
Pages
5051-7
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC247996
Subset
IM
Databases
GENBANK
M36502
External Links
PubMed source DOI: 10.1128/JVI.64.10.5051-5057.1990 PMC Full Text View journal details
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