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PMID: 7479833 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The nuclear matrix protein NMP-1 is the transcription factor YY1.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 92 ·No. 23 ·1995-11-07 ·Pages 10526-30

Guo B, Odgren PR, van Wijnen AJ, Last TJ, Nickerson J, Penman S, Lian JB, Stein JL, Stein GS

Abstract

NMP-1 was initially identified as a nuclear matrix-associated DNA-binding factor that exhibits sequence-specific recognition for the site IV regulatory element of a histone H4 gene. This distal promoter domain is a nuclear matrix interaction site. In the present study, we show that NMP-1 is the multifunctional transcription factor YY1. Gel-shift and Western blot analyses demonstrate that NMP-1 is immunoreactive with YY1 antibody. Furthermore, purified YY1 protein specifically recognizes site IV and reconstitutes the NMP-1 complex. Western blot and gel-shift analyses indicate that YY1 is present within the nuclear matrix. In situ immunofluorescence studies show that a significant fraction of YY1 is localized in the nuclear matrix, principally but not exclusively associated with residual nucleoli. Our results confirm that NMP-1/YY1 is a ubiquitous protein that is present in both human cells and in rat osteosarcoma ROS 17/2.8 cells. The finding that NMP-1 is identical to YY1 suggests that this transcriptional regulator may mediate gene-matrix interactions. Our results are consistent with the concept that the nuclear matrix may functionally compartmentalize the eukaryotic nucleus to support regulation of gene expression.

MeSH Terms
Animals Antibody Specificity Base Sequence Binding Sites Blotting, Western Cell Compartmentation Cell Nucleolus/chemistry,ultrastructure Cell Nucleus/chemistry,ultrastructure Cross Reactions DNA/metabolism DNA-Binding Proteins/genetics,immunology,isolation & purification,metabolism Erythroid-Specific DNA-Binding Factors HeLa Cells Humans Molecular Sequence Data Nuclear Matrix/chemistry Oligodeoxyribonucleotides/metabolism Protein Binding Rats Recombinant Proteins/isolation & purification,metabolism Transcription Factors/genetics,immunology,isolation & purification,metabolism YY1 Transcription Factor
Chemicals
DNA-Binding Proteins Erythroid-Specific DNA-Binding Factors Oligodeoxyribonucleotides Recombinant Proteins Transcription Factors YY1 Transcription Factor YY1 protein, human Yy1 protein, rat DNA
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Guo B
Department of Cell Biology, University of Masachusetts Medical Center, Worcester 01655, USA.
Odgren P R
van Wijnen A J
Last T J
Nickerson J
Penman S
Lian J B
Stein J L
Stein G S
References (53)
53 references, click to expand
  1. Chromosomal loop anchorage of the kappa immunoglobulin gene occurs next to the enhancer in a region containing topoisomerase II sites.
    Cell. 1986 Jan 31;44(2):273-82 PMID: 3002631
  2. RFP is a DNA binding protein associated with the nuclear matrix.
    Nucleic Acids Res. 1992 Oct 25;20(20):5305-10 PMID: 1437549
  3. Equipotent mouse ribosomal protein promoters have a similar architecture that includes internal sequence elements.
    Genes Dev. 1989 Nov;3(11):1789-800 PMID: 2606348
  4. Specific interactions of histone H1 and a 45 kilodalton nuclear protein with a putative matrix attachment site in the distal promoter region of a cell cycle-regulated human histone gene.
    J Cell Physiol. 1989 May;139(2):320-8 PMID: 2715190
  5. A pancreatic islet cell-specific enhancer-like element in the glucagon gene contains two domains binding distinct cellular proteins.
    J Biol Chem. 1990 May 25;265(15):8725-35 PMID: 2160464
  6. Transcriptional repression by YY1, a human GLI-Krüppel-related protein, and relief of repression by adenovirus E1A protein.
    Cell. 1991 Oct 18;67(2):377-88 PMID: 1655281
  7. Higher level organization of individual gene transcription and RNA splicing.
    Science. 1993 Feb 26;259(5099):1326-30 PMID: 8446901
  8. DNA-RNA complexes that might represent transient attachment sites of nuclear DNA to the matrix.
    J Cell Sci. 1990 Apr;95 ( Pt 4):667-74 PMID: 1696580
  9. Identification of a nuclear matrix-associated region of the c-myc protooncogene and its recognition by a nuclear protein in the human leukemia HL-60 cell line.
    Cancer Res. 1990 Jun 1;50(11):3199-206 PMID: 2185883
  10. The sequence-specific nuclear matrix binding factor F6 is a chicken GATA-like protein.
    Mol Gen Genet. 1993 Apr;238(3):309-14 PMID: 8492798
  11. Sequence-specific DNA-binding proteins are components of a nuclear matrix-attachment site.
    Proc Natl Acad Sci U S A. 1992 May 1;89(9):4178-82 PMID: 1570345
  12. TATA-binding protein-independent initiation: YY1, TFIIB, and RNA polymerase II direct basal transcription on supercoiled template DNA.
    Cell. 1994 Mar 25;76(6):1115-21 PMID: 8137426
  13. Tissue specific and position independent expression of the complete gene domain for chicken lysozyme in transgenic mice.
    EMBO J. 1990 Sep;9(9):2843-8 PMID: 2390972
  14. Estrogen receptors in the nuclear matrix: direct demonstration using monoclonal antireceptor antibody.
    Endocrinology. 1987 May;120(5):1851-7 PMID: 3569116
  15. Subnuclear distribution of the vitamin D receptor.
    J Cell Biochem. 1994 Apr;54(4):494-500 PMID: 8014199
  16. V-myc- and c-myc-encoded proteins are associated with the nuclear matrix.
    Mol Cell Biol. 1985 Jan;5(1):114-26 PMID: 3872410
  17. Nuclear factor 1 is a component of the nuclear matrix.
    J Cell Biochem. 1994 Jun;55(2):252-63 PMID: 8089200
  18. The nuclear matrix: a heuristic model for investigating genomic organization and function in the cell nucleus.
    J Cell Biochem. 1991 Oct;47(2):109-23 PMID: 1757479
  19. SnRNP core protein enrichment in the nuclear matrix.
    Biochem Biophys Res Commun. 1988 May 16;152(3):1383-7 PMID: 2967700
  20. A matrix/scaffold attachment region binding protein: identification, purification, and mode of binding.
    Cell. 1991 Jan 11;64(1):123-35 PMID: 1846084
  21. The nuclear matrix: defining structural and functional roles.
    Crit Rev Eukaryot Gene Expr. 1991;1(2):127-43 PMID: 1724928
  22. A tissue-specific MAR/SAR DNA-binding protein with unusual binding site recognition.
    Cell. 1992 Aug 21;70(4):631-45 PMID: 1505028
  23. A nuclear DNA attachment element mediates elevated and position-independent gene activity.
    Nature. 1989 Sep 28;341(6240):343-5 PMID: 2797152
  24. The nucleus: a black box being opened.
    J Cell Biochem. 1991 Dec;47(4):311-6 PMID: 1795015
  25. Chromatin architecture and nuclear RNA.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):177-81 PMID: 2911567
  26. Nuclear matrix localization and specific matrix DNA binding by receptor binding factor 1 of the avian oviduct progesterone receptor.
    Biochemistry. 1991 Oct 1;30(39):9516-22 PMID: 1892850
  27. Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold.
    Cell. 1984 Nov;39(1):223-32 PMID: 6091913
  28. Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
    Nucleic Acids Res. 1983 Mar 11;11(5):1475-89 PMID: 6828386
  29. U1 and U2 small nuclear RNAs are present in nuclear speckles.
    Proc Natl Acad Sci U S A. 1992 Jan 1;89(1):305-8 PMID: 1530887
  30. Interaction between transcription factors Sp1 and YY1.
    Nature. 1993 Sep 30;365(6445):462-4 PMID: 8003102
  31. DNA bending and orientation-dependent function of YY1 in the c-fos promoter.
    Genes Dev. 1993 Dec;7(12B):2497-509 PMID: 8276234
  32. An analysis of genes regulated by the multi-functional transcriptional regulator Yin Yang-1.
    Nucleic Acids Res. 1994 Dec 11;22(24):5151-5 PMID: 7816599
  33. The nonchromatin substructures of the nucleus: the ribonucleoprotein (RNP)-containing and RNP-depleted matrices analyzed by sequential fractionation and resinless section electron microscopy.
    J Cell Biol. 1986 May;102(5):1654-65 PMID: 3700470
  34. The retinoblastoma gene product is a cell cycle-dependent, nuclear matrix-associated protein.
    Proc Natl Acad Sci U S A. 1994 Jan 4;91(1):418-22 PMID: 8278403
  35. Cell structure and DNA organization.
    Crit Rev Eukaryot Gene Expr. 1991;1(4):355-85 PMID: 1802114
  36. Nuclear protein matrix: association with newly synthesized DNA.
    Science. 1975 Jul 25;189(4199):291-3 PMID: 1145202
  37. DNA supercoiling in chromatin structure and gene expression.
    Crit Rev Eukaryot Gene Expr. 1992;2(2):165-209 PMID: 1581628
  38. "Numatrin," a nuclear matrix protein associated with induction of proliferation in B lymphocytes.
    J Biol Chem. 1987 Aug 15;262(23):11389-97 PMID: 3301855
  39. Nuclear architecture supports integration of physiological regulatory signals for transcription of cell growth and tissue-specific genes during osteoblast differentiation.
    J Cell Biochem. 1994 May;55(1):4-15 PMID: 8083299
  40. The chicken lysozyme 5' matrix attachment region increases transcription from a heterologous promoter in heterologous cells and dampens position effects on the expression of transfected genes.
    Mol Cell Biol. 1990 May;10(5):2302-7 PMID: 2325653
  41. Major nucleolar proteins shuttle between nucleus and cytoplasm.
    Cell. 1989 Feb 10;56(3):379-90 PMID: 2914325
  42. Identification and characterization of an immunologically conserved adenovirus early region 11,000 Mr protein and its association with the nuclear matrix.
    J Mol Biol. 1982 Dec 15;162(3):565-83 PMID: 7166756
  43. Relief of YY1-induced transcriptional repression by protein-protein interaction with the nucleolar phosphoprotein B23.
    J Biol Chem. 1994 Mar 4;269(9):6506-10 PMID: 8120001
  44. Human-specific nuclear protein that associates with the polar region of the mitotic apparatus: distribution in a human/hamster hybrid cell.
    Cell. 1980 Nov;22(2 Pt 2):489-99 PMID: 7004645
  45. The Yin and the Yang of mammalian transcription.
    Curr Biol. 1992 Mar;2(3):152-4 PMID: 15335993
  46. Scaffold-attached regions from the human interferon beta domain can be used to enhance the stable expression of genes under the control of various promoters.
    Biochemistry. 1991 Feb 5;30(5):1264-70 PMID: 1991107
  47. Functional organization of the nuclear envelope.
    Annu Rev Cell Biol. 1988;4:335-74 PMID: 2461721
  48. Osteocalcin gene promoter-binding factors are tissue-specific nuclear matrix components.
    Proc Natl Acad Sci U S A. 1993 Apr 15;90(8):3162-6 PMID: 8475055
  49. Quantitative electrotransfer of proteins from sodium dodecyl sulfate-polyacrylamide gels onto positively charged nylon membranes.
    Anal Biochem. 1987 May 1;162(2):389-98 PMID: 2440346
  50. Inhibition of transcriptional regulator Yin-Yang-1 by association with c-Myc.
    Science. 1993 Dec 17;262(5141):1889-92 PMID: 8266081
  51. Nuclear matrix association of multiple sequence-specific DNA binding activities related to SP-1, ATF, CCAAT, C/EBP, OCT-1, and AP-1.
    Biochemistry. 1993 Aug 24;32(33):8397-402 PMID: 8357791
  52. Binding of a nuclear protein to the cyclic-AMP response element of the somatostatin gene.
    Nature. 1987 Jul 9-15;328(6126):175-8 PMID: 2885756
  53. Domains of the human androgen receptor and glucocorticoid receptor involved in binding to the nuclear matrix.
    J Cell Biochem. 1995 Mar;57(3):465-78 PMID: 7768981
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.92.23.10526
Published
1995-11-07
Pages
10526-30
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC40644
Subset
IM
Grants
NIAMS NIH HHS · AR42262 · United States
NIGMS NIH HHS · GM32010 · United States
External Links
PubMed source DOI: 10.1073/pnas.92.23.10526 PMC Full Text View journal details
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