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PMID: 7340837 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The alpha-adrenergic-mediated activation of Ca2+ influx into cardiac mitochondria. A possible mechanism for the regulation of intramitochondrial free CA2+.

The Biochemical journal ·Vol. 200 ·No. 2 ·1981-11-15 ·Pages 379-88

Kessar P, Crompton M

Abstract

Mitochondria isolated from rat hearts perfused with adrenaline, and from hearts excised from adrenaline-treated rats, showed an enhanced rate of respiration-dependent Ca2+ uptake. Adrenaline pretreatment did not change the activity of the Na+/Ca2+-antiporter of isolated heart mitochondria. Simultaneous measurements of the membrane potential revealed that perfusion with adrenaline has no significant effect on this parameter during Ca2+ accumulation. The activation of Ca2+ uptake was induced also by the alpha-adrenergic agonist, methoxamine, but not by the beta-adrenergic agonist, isoprenaline. Methoxamine pretreatment also increased the sensitivity of alpha-oxoglutarate dehydrogenase in intact mitochondria to 10 nM--300 nM extramitochondrial Ca2+ during steady-state Ca2+ recycling across the inner membrane. Possible implications of these data for the adrenergic regulation of oxidative metabolism are discussed.

MeSH Terms
Animals Biological Transport/drug effects Calcium/metabolism Epinephrine/pharmacology In Vitro Techniques Isoproterenol/pharmacology Membrane Potentials/drug effects Methoxamine/pharmacology Mitochondria, Heart/drug effects,metabolism Perfusion Rats Rats, Inbred Strains Sodium/metabolism
Chemicals
Sodium Methoxamine Isoproterenol Calcium Epinephrine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kessar P
Crompton M
References (32)
32 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2000379
Published
1981-11-15
Pages
379-88
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1163546
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2000379 PMC Full Text View journal details
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