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PMID: 7396840 Published · ppublish English Journal Article

The regulation of brain mitochondrial calcium-ion transport. The role of ATP in the discrimination between kinetic and membrane-potential-dependent calcium-ion efflux mechanisms.

The Biochemical journal ·Vol. 186 ·No. 3 ·1980-03-15 ·Pages 833-9

Nicholls DG, Scott ID

Abstract

Mitochondria from guinea-pig cerebral cortex incubated in the presence of Pi or acetate are unable to regulate the extramitochondrial free Ca2+ at a steady-state which is independent of the Ca2+ accumulated in the matrix. This is due to the superimposition on kinetically regulated Ca2+ cycling of a membrane-potential-dependent reversal of the Ca2+ uniporter. The latter efflux is a consequence of a low membrane potential, which correlates with a loss of adenine nucleotide loss from the matrix, enable the mitochondria to maintain a high membrane potential and allow the mitochondria to buffer the extramitochondrial free Ca2+ precisely when up to 200 nmol of Ca2+/mg of protein is accumulated in the matrix. The steady-state extramitochondrial free Ca2+ is maintained as low as 0.3 microM. The Na+-activated efflux pathway is functional in the presence of ATP and oligomycin and accounts precisely for the change in steady-state free Ca2+ induced by Na+ addition. The need to distinguish carefully between kinetic and membrane-potential-dependent efflux pathways is emphasized and the competence of brain mitochondria to regulate cytosolic free Ca2+ concentrations in vivo is discussed.

MeSH Terms
Adenosine Triphosphate/pharmacology,physiology Animals Atractyloside/pharmacology Biological Transport/drug effects Calcium/metabolism Cerebral Cortex/drug effects,metabolism Female Guinea Pigs In Vitro Techniques Kinetics Male Membrane Potentials/drug effects Mitochondria/drug effects,metabolism Oligomycins/pharmacology Sodium/pharmacology
Chemicals
Oligomycins Atractyloside Adenosine Triphosphate Sodium Calcium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Nicholls D G
Scott I D
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38 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1860833
Published
1980-03-15
Pages
833-9
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1161720
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1860833 PMC Full Text View journal details
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