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PMID: 7050077 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Expression of a copy number control gene (copB) of plasmid R1 is constitutive and growth rate dependent.

Journal of bacteriology ·Vol. 151 ·No. 3 ·1982-09-00 ·Pages 1129-35

Light J, Molin S

Abstract

The copy number control gene copB from plasmid R1 was fused to the lacZ gene in vitro, resulting in expression of a fused polypeptide consisting of the first 53 amino acids of the CopB polypeptide and the beta-galactosidase polypeptide minus its first 8 amino acids. Based on measurements of specific activities of this fused protein under various conditions, it was concluded that expression of copB is gene dosage dependent, unregulated by plasmid-coded functions, and proportional to growth rate between 0.4 and 2.0 doublings per h. The rate of expression of the copB gene is surprisingly high compared with other known cases of regulatory proteins.

MeSH Terms

Bacterial Proteins/genetics DNA Replication DNA, Recombinant Escherichia coli/genetics,growth & development Gene Expression Regulation Genes, Regulator Operon R Factors

Chemicals

Bacterial Proteins DNA, Recombinant

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Light J

Molin S

References (27)

27 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.151.3.1129-1135.1982

Published

1982-09-00

Pages

1129-35

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC220388

Subset

External Links

PubMed source DOI: 10.1128/jb.151.3.1129-1135.1982 PMC Full Text View journal details

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Expression of a copy number control gene (copB) of plasmid R1 is constitutive and growth rate dependent.

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