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PMID: 7045074 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Effects of the ssb-1 and ssb-113 mutations on survival and DNA repair in UV-irradiated delta uvrB strains of Escherichia coli K-12.

Journal of bacteriology ·Vol. 151 ·No. 1 ·1982-07-00 ·Pages 186-92

Wang TC, Smith KC

Abstract

The molecular defect in DNA repair caused by ssb mutations (single-strand binding protein) was studied by analyzing DNA synthesis and DNA double-strand break production in UV-irradiated Escherichia coli delta uvrB strains. The presence of the ssb-113 mutation produced a large inhibition of DNA synthesis and led to the formation of double-strand breaks, whereas the ssb-1 mutation produced much less inhibition of DNA synthesis and fewer double-strand breaks. We suggest that the single-strand binding protein plays an important role in the replication of damaged DNA, and that it functions by protecting single-stranded parental DNa opposite daughter-strand gaps from nuclease attack.

MeSH Terms
Cell Survival/radiation effects DNA Repair DNA Replication/radiation effects Dose-Response Relationship, Radiation Escherichia coli/genetics,radiation effects Genotype Kinetics Mutation Species Specificity Ultraviolet Rays
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Wang T C
Smith K C
References (29)
29 references, click to expand
  1. Three loci in Escherichia coli K-12 that control the excision of pyrimidine dimers and certain other mutagen products from DNA.
    Genetics. 1966 Jun;53(6):1119-36 PMID: 5335128
  2. Discontinuities in the DNA synthesized in an excision-defective strain of Escherichia coli following ultraviolet irradiation.
    J Mol Biol. 1968 Jan 28;31(2):291-304 PMID: 4865486
  3. DNA repair.
    Annu Rev Biochem. 1968;37:175-200 PMID: 4875714
  4. Dark recovery processes in Escherichia coli irradiated with ultraviolet light. I. Effect of rec mutations on liquid holding recovery.
    J Bacteriol. 1968 Aug;96(2):365-73 PMID: 4877124
  5. Studies on radiation-sensitive mutants of E. coli. II. Breakage and repair of ultraviolet irradiated intracellular DNA of phage lambda.
    Mol Gen Genet. 1968 May 3;101(3):245-56 PMID: 4879098
  6. The pitfalls of measuring DNA synthesis kinetics as exemplifed in ultraciolet radiation studies.
    Biochim Biophys Acta. 1968 Dec 17;169(2):430-8 PMID: 4883324
  7. DNA synthesis in sensitive and resistant mutants of Escherichia coli B after ultraviolet irradiation.
    Mutat Res. 1969 Nov-Dec;8(3):481-95 PMID: 4905740
  8. Deoxyribonucleic acid replication in UV-damaged bacteria revisited.
    Mutat Res. 1971 Jun;12(2):121-8 PMID: 4937686
  9. Exchanges between DNA strands in ultraviolet-irradiated Escherichia coli.
    J Mol Biol. 1971 Oct 14;61(1):25-44 PMID: 4947693
  10. exrB: a malB-linked gene in Escherichia coli B involved in sensitivity to radiation and filament formation.
    Genet Res. 1974 Apr;23(2):175-84 PMID: 4607513
  11. Conversion of phiX174 viral DNA to double-stranded form by purified Escherichia coli proteins.
    Proc Natl Acad Sci U S A. 1974 Oct;71(10):4120-4 PMID: 4610569
  12. Enzymatic production of deoxyribonucleic acid double-strand breaks after ultraviolet irradiation of Escherichia coli K-12.
    J Bacteriol. 1975 Feb;121(2):511-7 PMID: 1089633
  13. The deoxyribonucleic acid unwinding protein of Escherichia coli. Properties and functions in replication.
    J Biol Chem. 1975 Mar 25;250(6):1972-80 PMID: 1090613
  14. Reversion studies with exrB in Escherichia coli.
    Genet Res. 1975 Apr;25(2):109-17 PMID: 1102387
  15. Genetic control of multiple pathways of post-replicational repair in uvrB strains of Escherichia coli K-12.
    J Bacteriol. 1976 Jan;125(1):102-10 PMID: 1107301
  16. A mechanism of duplex DNA replication revealed by enzymatic studies of phage phi X174: catalytic strand separation in advance of replication.
    Proc Natl Acad Sci U S A. 1977 Jan;74(1):193-7 PMID: 138139
  17. Repair of DNA double-strand breaks in Escherichia coli, which requires recA function and the presence of a duplicate genome.
    J Mol Biol. 1977 Oct 15;116(1):81-98 PMID: 338918
  18. Genetic mapping of the lexC-113 mutation.
    Mol Gen Genet. 1977 Nov 29;157(1):91-7 PMID: 340916
  19. Reconstitution of an Escherichia coli repair endonuclease activity from the separated uvrA+ and uvrB+/uvrC+ gene products.
    Proc Natl Acad Sci U S A. 1978 Jun;75(6):2569-73 PMID: 351611
  20. An Escherichia coli mutant defective in single-strand binding protein is defective in DNA replication.
    Proc Natl Acad Sci U S A. 1979 Apr;76(4):1702-5 PMID: 221903
  21. Mutant single-strand binding protein of Escherichia coli: genetic and physiological characterization.
    J Bacteriol. 1979 Oct;140(1):14-9 PMID: 227832
  22. A temperature-sensitive single-stranded DNA-binding protein from Escherichia coli.
    J Biol Chem. 1980 Apr 10;255(7):2897-901 PMID: 6244299
  23. Linkage map of Escherichia coli K-12, edition 6.
    Microbiol Rev. 1980 Mar;44(1):1-56 PMID: 6997720
  24. Synthesis of recA protein and induction of bacteriophage lambda in single-strand deoxyribonucleic acid-binding protein mutants of Escherichia coli.
    J Bacteriol. 1980 Nov;144(2):489-98 PMID: 6448838
  25. Effect of ssbA1 and lexC113 mutations on lambda prophage induction, bacteriophage growth, and cell survival.
    J Bacteriol. 1980 Aug;143(2):887-96 PMID: 6451615
  26. The effects of lexA101, recB21, recF143 and uvrD3 mutations on liquid-holding recovery in ultraviolet-irradiated Escherichia coli K12 recA56.
    Mutat Res. 1981 Jan;80(1):15-25 PMID: 7010131
  27. Repair of DNA double-strand breaks in Escherichia coli cells requires synthesis of proteins that can be induced by UV light.
    Proc Natl Acad Sci U S A. 1981 Jun;78(6):3450-3 PMID: 7022449
  28. DNA repair in E. coli strains deficient in single-strand DNA binding protein.
    Mol Gen Genet. 1981;183(2):341-7 PMID: 6276685
  29. Variable expression of the ssb--1 allele in different strains of Escherichia coli K12 and B: differential suppression of its effects on DNA replication, DNA repair and ultraviolet mutagenesis.
    Mol Gen Genet. 1981;183(2):348-55 PMID: 6276686
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.151.1.186-192.1982
Published
1982-07-00
Pages
186-92
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC220225
Subset
IM
Grants
NCI NIH HHS · CA-02896 · United States
NCI NIH HHS · CA-10372 · United States
External Links
PubMed source DOI: 10.1128/jb.151.1.186-192.1982 PMC Full Text View journal details
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