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PMID: 7035333 Published · ppublish English Journal Article Review

The application of DNA recombinant technology to the analysis of the human genome and genetic disease.

Human genetics ·Vol. 58 ·No. 4 ·1981-00-00 ·Pages 351-7

Davies KE

Abstract

Recombinant DNA technology permits the isolation of libraries of DNA sequences corresponding to either the whole genome of an individual or the expressed sequences of a given cell type. Gene-specific probes isolated from these libraries may be used for the identification of DNA sequences in the genome necessary for normal gene function and for the study of the consequences of mutations and rearrangements in these sequences which give rise to the clinical symptoms in genetic disease. DNA sequence polymorphisms can be used to construct a genetic linkage map of the entire human genome. This allows the development of antenatal diagnoses for monogenic diseases even in the absence of an understanding of the biochemical defect.

MeSH Terms
Base Sequence Chromosome Aberrations Chromosome Mapping Chromosomes, Human/ultrastructure Containment of Biohazards DNA/analysis DNA, Recombinant Genes Genetic Diseases, Inborn/genetics Humans
Chemicals
DNA, Recombinant DNA
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Davies K E
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55 references, click to expand
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Article Info
Journal
Human genetics
Abbr.
Hum Genet
ISSN
0340-6717
DOI
10.1007/BF00282814
Published
1981-00-00
Pages
351-7
Language
English
Region
Germany
NLM ID
7613873
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00282814 View journal details
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