Home LiteratureArticle Details
PMID: 7021538 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Molecular mechanisms of pyrimidine dimer excision in Saccharomyces cerevisiae: excision of dimers in cell extracts.

Journal of bacteriology ·Vol. 147 ·No. 2 ·1981-08-00 ·Pages 705-8

Reynolds RJ, Love JD, Friedberg EC

Abstract

Cell-free extracts prepared from rad1-19, rd2-2, rad3-1, rad4-3, rad7-1, rad10-1, rd14-1, rad16-1, and cyc1-1 (rad7) mutants of Saccharomyces cerevisiae all catalyze the preferential excision of thymine-containing pyrimidine dimers from ultraviolet-irradiated DNA specifically incised with M. luteus ultraviolet deoxyribonucleic acid incising activity.

MeSH Terms
Cell-Free System DNA, Fungal/metabolism Mutation Pyrimidine Dimers/metabolism Saccharomyces cerevisiae/genetics,metabolism Thymine/metabolism Ultraviolet Rays
Chemicals
DNA, Fungal Pyrimidine Dimers Thymine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Reynolds R J
Love J D
Friedberg E C
References (23)
23 references, click to expand
  1. Three additional genes involved in pyrimidine dimer removal in Saccharomyces cerevisiae: RAD7, RAD14 and MMS19.
    Mol Gen Genet. 1979 Nov;176(3):351-9 PMID: 392238
  2. Ultraviolet-endonuclease activity in cell extracts of Saccharomyces cerevisiae mutants defective in excision of pyrimidine dimers.
    J Bacteriol. 1980 Apr;142(1):322-4 PMID: 6246068
  3. A new mutant of the yeast Saccharomyces cerevisiae defective in excision of UV-damaged sites in DNA.
    Mol Gen Genet. 1980 Feb;177(3):541-4 PMID: 6990205
  4. Cleavage of pyrimidine dimers in specific DNA sequences by a pyrimidine dimer DNA-glycosylase of M. luteus.
    Nature. 1980 Jun 26;285(5767):634-41 PMID: 6248789
  5. Molecular mechanisms of pyrimidine dimer excision in Saccharomyces cerevisiae: incision of ultraviolet-irradiated deoxyribonucleic acid in vivo.
    J Bacteriol. 1981 May;146(2):692-704 PMID: 7012136
  6. DNA repair in bacteria and mammalian cells.
    Annu Rev Biochem. 1979;48:783-836 PMID: 382997
  7. Deletions of the iso-1-cytochrome c and adjacent genes of yeast: discovery of the OSM1 gene controlling osmotic sensitivity.
    Genetics. 1978 Aug;89(4):653-65 PMID: 211068
  8. Saccharomyces cerevisiae cell cycle mutant cdc9 is defective in DNA ligase.
    Nature. 1978 Aug 31;274(5674):891-3 PMID: 355897
  9. Removal of pyrimidine dimers from Saccharomyces cerevisiae nuclear DNA under nongrowth conditions as detected by a sensitive, enzymatic assay.
    Mutat Res. 1978 Apr;50(1):43-56 PMID: 347280
  10. Repair of pyrimidine dimers in radiation-sensitive mutants rad3, rad4, rad6 and rad9 of Saccharomyces cerevisiae.
    Mutat Res. 1977 Oct;45(1):13-20 PMID: 335235
  11. Defective thymine dimer excision in radiation-sensitive mutants rad10 and rad16 of Saccharomyces cerevisiae.
    Mol Gen Genet. 1977 Apr 29;152(3):125-8 PMID: 327268
  12. A simple method for the large-scale preparation of mitochondria from microorganisms.
    Anal Biochem. 1977 Jan;77(1):110-21 PMID: 137681
  13. Repair of pyrimidine dimers in nuclear and mitochondrial DNA of yeast irradiated with low doses of ultraviolet light.
    J Mol Biol. 1975 Nov 15;98(4):781-95 PMID: 1104879
  14. Action of exonuclease V (the recBC enzyme) on ultraviolet-irradiated DNA.
    Biochim Biophys Acta. 1975 Mar 10;383(2):178-87 PMID: 1091299
  15. Deoxyribonucleic acid polymerase III of Escherichia coli. Characterization of associated exonuclease activities.
    J Biol Chem. 1975 Jan 25;250(2):470-8 PMID: 163228
  16. The disappearance of ultraviolet-induced pyrimidine dimers from the nuclear DNA of exponential and stationary phase cells of Saccharomyces cerevisiae following various post-irradiation treatments.
    Biochim Biophys Acta. 1974 Jul 24;353(4):407-19 PMID: 4605235
  17. Exonuclease VII of Escherichia coli. Mechanism of action.
    J Biol Chem. 1974 Jul 25;249(14):4553-61 PMID: 4602030
  18. Excision of thymine dimers in vitro by extracts of bacteriophage-infected Escherichia coli.
    J Virol. 1974 May;13(5):953-9 PMID: 4596298
  19. The excision of pyrimidine dimers from DNA of ultraviolet irradiated yeast.
    Mol Gen Genet. 1971;113(4):359-62 PMID: 4947372
  20. Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae.
    Mol Gen Genet. 1979;172(3):249-58 PMID: 45608
  21. The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast.
    Mutat Res. 1968 Jul-Aug;6(1):37-55 PMID: 5708072
  22. Mutants of yeast sensitive to ultraviolet light.
    J Bacteriol. 1967 Sep;94(3):571-5 PMID: 6035260
  23. Repair of pyrimidine dimer damage induced in yeast by ultraviolet light.
    J Bacteriol. 1972 Mar;109(3):979-86 PMID: 4551759
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.147.2.705-708.1981
Published
1981-08-00
Pages
705-8
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC216098
Subset
IM
Grants
NCI NIH HHS · CA-06441 · United States
NCI NIH HHS · CA-12428 · United States
External Links
PubMed source DOI: 10.1128/jb.147.2.705-708.1981 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2