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PMID: 45608 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Decreased UV mutagenesis in cdc8, a DNA replication mutant of Saccharomyces cerevisiae.

Molecular & general genetics : MGG ·Vol. 172 ·No. 3 ·1979-00-00 ·Pages 249-58

Prakash L, Hinkle D, Prakash S

Abstract

A DNA replication mutant of yeast, cdc8, was found to decrease UV-induced reversion of lys2-1, arg4-17, tyr1 and ura1. This effect was observed with all three alleles of cdc8 tested. Survival curves obtained following UV irradiation in cdc8 rad double mutants show that cdc8 is epistatic to rad6, as well as to rad1; cdc8 rad51 double mutants seem to be more sensitive than the single mutants. Since UV-induced reversion in cdc8 rad1 and cdc8 rad51 double mutants is like that of the cdc8 single mutants, we conclude that CDC8 plays a direct role in error-prone repair. To test whether CDC8 codes for a DNA polymerase, we have purified both DNA polymerase I and DNA polymerase II from cdc8 and CDC+ cells. The purified DNA polymerases from cdc8 were no more heat labile than those from CDC+, suggesting that CDC8 is not a structural gene for either enzyme.

MeSH Terms
Alleles DNA Replication/genetics DNA, Fungal/biosynthesis,genetics,radiation effects DNA-Directed DNA Polymerase/genetics,isolation & purification Dose-Response Relationship, Radiation Genes, Fungal/radiation effects Mutagenesis Radiation Tolerance/genetics Saccharomyces cerevisiae/genetics,radiation effects Ultraviolet Rays
Chemicals
DNA, Fungal DNA-Directed DNA Polymerase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Prakash L
Department of Radiation Biology and Biophysics, University of Rochester, School of Medicine and Dentistry, New York 14642.
Hinkle D
Prakash S
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00271724
Published
1979-00-00
Pages
249-58
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM 19261 · United States
NIGMS NIH HHS · KO4 GM-00004 · United States
NIGMS NIH HHS · KO4 GM-0021 · United States
External Links
PubMed source DOI: 10.1007/BF00271724
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