A new mutant of the yeast Saccharomyces cerevisiae defective in excision of UV-damaged sites in DNA.

Molecular & general genetics : MGG ·Vol. 177 ·No. 3 ·1980-02-00 ·Pages 541-4

Bekker ML, Kaboev OK, Koval'tsova SV

Abstract

An UV-sensitive yeast mutant, uvs12, with almost unchanged sensitivity to gamma-irradiation and methylmethane sulphonate was obtained. uvs12, non allelic to any of the known UV-sensitive mutants from radI to rad21 is defective in early steps of excision repair. This inference is based on the fact that after 4-5 h post-irradiation incubation unexcised pyrimidine dimers are retained in nuclear DNA, which follows from two independent tests: the retention of UV-endonuclease-sensitive sites and enhanced survival after photoreactivation.

MeSH Terms

DNA Repair/radiation effects DNA, Fungal/genetics,radiation effects Gamma Rays Light Mutation Phenotype Pyrimidine Dimers/radiation effects Saccharomyces cerevisiae/genetics Ultraviolet Rays

Chemicals

DNA, Fungal Pyrimidine Dimers

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bekker M L

Kaboev O K

Koval'tsova S V

References (8)

8 references, click to expand

Defective thymine dimer excision in radiation-sensitive mutants rad10 and rad16 of Saccharomyces cerevisiae.
Mol Gen Genet. 1977 Apr 29;152(3):125-8 PMID: 327268
Interactions among genes controlling sensitivity to radiation and alkylation in yeast.
Mol Gen Genet. 1973 Sep 12;125(3):197-216 PMID: 4359562
SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
The isolation, genetics and survival characteristics of ultraviolet light-sensitive mutants in yeast.
Mutat Res. 1968 Jul-Aug;6(1):37-55 PMID: 5708072
Repair of pyrimidine dimers in radiation-sensitive mutants rad3, rad4, rad6 and rad9 of Saccharomyces cerevisiae.
Mutat Res. 1977 Oct;45(1):13-20 PMID: 335235
Sedimentation properties of yeast chromosomal DNA.
Proc Natl Acad Sci U S A. 1972 May;69(5):1188-91 PMID: 4556456
Repair systems in Saccharomyces.
Mutat Res. 1974 Aug;26(4):257-64 PMID: 4605044
Repair of pyrimidine dimers in nuclear and mitochondrial DNA of yeast irradiated with low doses of ultraviolet light.
J Mol Biol. 1975 Nov 15;98(4):781-95 PMID: 1104879

Article Info

Journal

Molecular & general genetics : MGG

Abbr.

Mol Gen Genet

ISSN

0026-8925

DOI

10.1007/BF00271495

Published

1980-02-00

Pages

541-4

Language

English

Region

Germany

NLM ID

0125036

Subset

External Links

PubMed source DOI: 10.1007/BF00271495

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A new mutant of the yeast Saccharomyces cerevisiae defective in excision of UV-damaged sites in DNA.

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