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PMID: 6997489 Published · ppublish English Journal Article Review

The use of isolated membrane vesicles to study epithelial transport processes.

The Journal of membrane biology ·Vol. 55 ·No. 2 ·1980-07-15 ·Pages 81-95

Murer H, Kinne R

Abstract

Epithelia are multicompartment and multicomponent systems performing transcellular and paracellular transport in a very complex manner. One way to get a deeper understanding of the function of such a complex system is to dissect it into the single components and then, after having defined the components under well-controlled conditions, to try to describe the behavior of the whole system on the basis of the properties of the single components. This article deals with the analysis of isolated plasma membranes derived from the luminal and contraluminal face of epithelial cells, predominantly renal proximal tubular and small intestinal cells. It is aimed to give an overview of methods used to isolate and separate plasma membranes, to study their transport properties as membrane vesicles, and also to address the question of how information gained with the isolated membranes corresponds to observations made in the intact cell using other, notably electrophysiological, measurements. The review also critically evaluates the limitations of the approach and thereby tries to set the work on isolated membranes in the proper perspective within the field of transport physiology.

MeSH Terms
Animals Biological Transport Cell Membrane/metabolism Cell-Free System Epithelium/metabolism Intestinal Mucosa/metabolism Kidney Tubules/metabolism Kinetics Microvilli/metabolism Models, Biological Sodium/metabolism
Chemicals
Sodium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Murer H
Kinne R
References (84)
84 references, click to expand
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Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01871151
Published
1980-07-15
Pages
81-95
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01871151 View journal details
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