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PMID: 435284 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

L-proline transport by newborn rat kidney brush-border membrane vesicles.

The Biochemical journal ·Vol. 178 ·No. 1 ·1979-01-15 ·Pages 253-6

Goldmann DR, Roth KS, Langfitt TW, Segal S

Abstract

The transport of L-proline was studied in brush-border membrane vesicles isolated from the kidneys of newborn rats. In contrast with the rapid initial uptake with an 'overshoot' observed in adult vesicles, uptake by the newborn vesicle was slow, showed no 'overshoot', and proline continued to accumulate at a time when the adult vesicle had already equilibrated. L-Proline transport in the newborn rat occurs by Na+-dependent and independent mechanisms. There appeared to be essentially no uptake by anti-luminal vesicles isolated from newborn rat kidney. These observations may help to explain the prolinuria that occurs in the newborn animal.

MeSH Terms

Animals Animals, Newborn/metabolism Biological Transport In Vitro Techniques Kidney Cortex/metabolism Microvilli/metabolism Proline/metabolism Rats Sodium/metabolism

Chemicals

Proline Sodium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Goldmann D R

Roth K S

Langfitt T W

Segal S

References (12)

12 references, click to expand

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Transport of amino acids in renal brush border membrane vesicles. Uptake of L-proline.
J Biol Chem. 1977 Jan 25;252(2):591-5 PMID: 833146
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Isolation and characterization of the brush border fraction from newborn rat renal proximal tubule cells.
Biochim Biophys Acta. 1976 Jan 21;419(2):251-60 PMID: 2293
Metabolism and uptake of l-proline by human kidney cortex.
Pediatr Res. 1973 Oct;7(10):818-25 PMID: 4747312
Separate transport systems for sugars and amino acids in developing rat kidney cortex.
Proc Natl Acad Sci U S A. 1971 Feb;68(2):372-6 PMID: 5277088
The ontogeny of amino acid transport in rat kidney. II. Kinetics of uptake and effect of anoxia.
Biochim Biophys Acta. 1971 Dec 3;249(2):364-72 PMID: 5134189
The ontogeny of amino acid transport in rat kidney. I. Effect on distribution ratios and intracellular metabolism of proline and glycine.
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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1780253

Published

1979-01-15

Pages

253-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1186506

Subset

External Links

PubMed source DOI: 10.1042/bj1780253 PMC Full Text View journal details

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L-proline transport by newborn rat kidney brush-border membrane vesicles.

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