The polarity of the proximal tubule cell in rat kidney. Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings.

The Journal of cell biology ·Vol. 54 ·No. 2 ·1972-08-00 ·Pages 232-45

Heidrich HG, Kinne R, Kinne-Saffran E, Hannig K

Abstract

Two different membrane fractions were obtained from a brush-border fraction of rat kidney cortex by using their different electrical surface charges in preparative free-flow electrophoresis. One membrane fraction contained only morphologically intact microvilli and was characterized by a high specific activity of alkaline phosphatase. The other fraction morphologically resembled classical plasma membranes by possessing junctional complexes and a high Na-K-ATPase activity The contamination of the isolated membrane fractions by other cell organelles was extremely low These two fractions represent the apical (luminal) and the basal (interstitial) area of the renal proximal tubule cell membrane and clearly demonstrate the polarity of this cell.

MeSH Terms

Adenosine Triphosphatases/metabolism Alkaline Phosphatase/metabolism Animals Biological Transport, Active Cell Membrane/enzymology,physiology Intercellular Junctions Kidney Tubules/cytology,physiology Male Membrane Potentials Microscopy, Electron Potassium Rats Sodium

Chemicals

Sodium Alkaline Phosphatase Adenosine Triphosphatases Potassium

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Heidrich H G

Kinne R

Kinne-Saffran E

Hannig K

References (13)

13 references, click to expand

Combined biochemical and morphological study of particulate fractions from rat liver. Analysis of preparations enriched in lysosomes or in particles containing urate oxidase, D-amino acid oxidase, and catalase.
J Cell Biol. 1965 Jul;26(1):219-43 PMID: 4379260
On the localization of some phosphatases in three different segments of the proximal tubules in the rat kidney.
J Histochem Cytochem. 1967 Aug;15(8):456-69 PMID: 4293010
The role of sodium-potassium-activated adenosine triphosphatase in the reabsorption of sodium by the kidney.
J Clin Invest. 1967 Dec;46(12):1999-2011 PMID: 4294572
Rat intestinal microvillus membranes. Purification and biochemical characterization.
Biochem J. 1968 Jan;106(2):381-90 PMID: 4295331
Brush border particulates of renal tissue.
Science. 1968 Nov 29;162(3857):1009-11 PMID: 4301646
[Protein absorption droplets and lysosomes in the proximal convoluted tubule of the rat kidney. Combined ectronmicroscopic and enzyme-cytochemical studies using intratubular injections with ferritin].
Virchows Arch B Cell Pathol. 1970;5(2):124-43 PMID: 4986582
The isolation of a brush border membrane fraction from rat kidney.
J Biol Chem. 1970 Nov 25;245(22):6106-12 PMID: 4320796
Isolation and biochemical characterization of brush borders from rabbit kidney.
J Cell Biol. 1970 Dec;47(3):637-45 PMID: 4250612
The localization of the Na + -K + -ATPase in the cells of rat kidney cortex. A study on isolated plasma membranes.
Pflugers Arch. 1971;329(3):191-206 PMID: 4333831
Studies on the arrangement of aminopeptidase and alkaline phosphatase in the microvilli of isolated brush border of rat kidney.
Biochim Biophys Acta. 1972 Jan 17;255(1):114-25 PMID: 5010988
[Morphological and biochemical studies on the surface structure of the rat kidney brush-border membrane].
Pflugers Arch. 1972;333(4):297-313 PMID: 4115672
A new method for the preparation of rat liver lysosomes. Separation of cell organelles of rat liver by carrier-free continuous electrophoresis.
J Cell Biol. 1970 Sep;46(3):576-91 PMID: 4349132
ENZYMATIC BASIS FOR ACTIVE TRANSPORT OF NA+ AND K+ ACROSS CELL MEMBRANE.
Physiol Rev. 1965 Jul;45:596-617 PMID: 14337569

Article Info

Journal

The Journal of cell biology

Abbr.

J Cell Biol

ISSN

0021-9525

DOI

10.1083/jcb.54.2.232

Published

1972-08-00

Pages

232-45

Language

English

Region

United States

NLM ID

0375356

PMCID

PMC2108866

Subset

External Links

PubMed source DOI: 10.1083/jcb.54.2.232 PMC Full Text View journal details

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The polarity of the proximal tubule cell in rat kidney. Different surface charges for the brush-border microvilli and plasma membranes from the basal infoldings.

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