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PMID: 6975237 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for two populations of B-L (Ia-like) molecules encoded by the chicken MHC.

Immunogenetics ·Vol. 13 ·No. 5 ·1981-00-00 ·Pages 381-91

Crone M, Jensenius J, Koch C

Abstract

Two specific alloantisera detecting B-L (Ia-like) antigens on chicken lymphocytes of the B6 and B15 haplotypes were found to cross-react strongly. Anti-B-L6 and anti-B-L15 alloantisera both reacted with B-L molecules on B6 and B15 lymphocytes as demonstrated by immunofluorescence and SDS-PAGE analysis of 125I-labeled B-L antigens isolated by incubation with anti-B-L alloantisera. Absorption studies showed that the anti-B-L alloantisera reacted with at least two kinds of antigenic determinant, one set shared by B-L6 and B-L15 molecules and another set specific for each haplotype. In spite of the absence of genetic evidence for more than one B-L locus in the chicken B complex, it was shown by sequential antibody incubations that these two different B-L antigenic determinants are associated with at least two separate species of B-L molecules, indicating the presence of at least two B-L loci within the MHC of the chicken.

MeSH Terms
Animals B-Lymphocytes/immunology Chickens/genetics Chromosome Mapping Electrophoresis, Polyacrylamide Gel Genes Histocompatibility Antigens Class II/genetics Major Histocompatibility Complex T-Lymphocytes/immunology
Chemicals
Histocompatibility Antigens Class II
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Crone M
Jensenius J
Koch C
References (29)
29 references, click to expand
  1. Structural and serological properties of murine Ia alloantigens.
    Transplant Rev. 1976;30:236-70 PMID: 59974
  2. The H-2 major histocompatibility complex and the I immune response region: genetic variation, function, and organization.
    Adv Immunol. 1975;20:125-95 PMID: 47219
  3. Transplantation in miniature swine. V. Characterization of Ia antigens.
    J Immunol. 1979 Feb;122(2):623-7 PMID: 368245
  4. Two-gene control of the expression of a murine Ia antigen.
    J Exp Med. 1978 Oct 1;148(4):925-39 PMID: 100572
  5. T lymphocyte-enriched murine peritoneal exudate cells. III. Inhibition of antigen-induced T lymphocyte Proliferation with anti-Ia antisera.
    J Immunol. 1976 Aug;117(2):531-40 PMID: 1084902
  6. Monoclonal antibody against an Ir gene product?
    J Exp Med. 1980 Oct 1;152(4):1085-101 PMID: 6158546
  7. Labeling characteristics and separation of Ia antigen subunits.
    J Immunol. 1978 Feb;120(2):671-5 PMID: 621402
  8. Evidence for an association between immune responsiveness of chicken to (T, G)-A--L and the major histocompatibility system.
    Eur J Immunol. 1974 Aug;4(8):548-53 PMID: 4413330
  9. Structural characteristics of the alloantigens determined by the major histocompatibility complex of the guinea pig.
    J Exp Med. 1976 Mar 1;143(3):541-58 PMID: 55459
  10. Analysis of H-2 and Ia molecules by two-dimensional gel electrophoresis.
    J Exp Med. 1977 Nov 1;146(5):1261-79 PMID: 411873
  11. The mixed leukocyte reaction in chickens. Evidence for control by the major histocompatibility complex.
    Eur J Immunol. 1974 Jun;4(6):397-401 PMID: 4278328
  12. Sharing of Ia antigens between species. II. Molecular localization of shared Ia determinants implies the existence of more than one I sublocus of the rat MHC.
    J Immunol. 1978 Aug;121(2):637-40 PMID: 79611
  13. Linkage disequilibrium of MHC genes in the chicken. I. The B-F and B-G loci.
    Immunogenetics. 1980;10(2):103-12 PMID: 7419245
  14. Genetic regions of controlling immune responsiveness in primates.
    Transplant Proc. 1977 Mar;9(1):837-45 PMID: 68605
  15. Structural studies on the murine Ia alloantigens. VI. Evidence that both subunits of the I-A alloantigen are encoded by the I-A subregion.
    J Immunol. 1979 Dec;123(6):2799-803 PMID: 501089
  16. Ia-like alloantigens in the chicken: Serologic characterization an ontogeny of cellular expression.
    Immunogenetics. 1978 Dec;7(1):521-35 PMID: 21312021
  17. Organization of the immune response genes.
    Science. 1979 Oct 19;206(4416):292-7 PMID: 113876
  18. Recombination between genes coding for immune response and the serologically determined antigens in the chickenB system.
    Immunogenetics. 1978 Dec;7(1):25-33 PMID: 21302053
  19. A micromethod of chicken MLR: technical aspects and genetic control.
    Tissue Antigens. 1980 Apr;15(4):373-80 PMID: 6451047
  20. Structural studies on the murine Ia alloantigens--III. Tryptic peptide comparisons of allelic products of the I-E/C sub-region.
    Mol Immunol. 1979 Jan;16(1):29-35 PMID: 447366
  21. Structure of Ia antigens from the rat. Mouse alloantisera demonstrate at least two distinct molecular species.
    Eur J Immunol. 1980 Feb;10(2):145-51 PMID: 6154583
  22. Allo-aggression in chickens: analysis of the B-complex by means of GVH splenomegaly and by inhibitory antibodies.
    Adv Exp Med Biol. 1977;88:267-74 PMID: 21545
  23. Inhibition of immune responses in vitro by specific antiserums to Ia antigens.
    Science. 1975 Apr 18;188(4185):268-70 PMID: 1118728
  24. On multiple alleles effecting cellular antigens in the chicken.
    Genetics. 1950 Nov;35(6):633-52 PMID: 14793708
  25. Marek's disease: effects of B histocompatibility alloalleles in resistant and susceptible chicken lines.
    Science. 1977 Jan 14;195(4274):193-5 PMID: 831269
  26. Restricted expression of an MHC alloantigen in cells of the erythroid series: a specific marker for erythroid differentiation.
    J Supramol Struct. 1980;13(3):395-400 PMID: 7206712
  27. The guinea pig I region. I. A structural and genetic analysis.
    J Exp Med. 1977 Aug 1;146(2):547-60 PMID: 69004
  28. Sharing of Ia antigens between species. I. Detection of Ia specificities shared by rats and mice.
    J Exp Med. 1977 Aug 1;146(2):381-93 PMID: 68999
  29. The elusive T cell receptor.
    Transplant Rev. 1972;10:36-56 PMID: 4403900
Article Info
Journal
Immunogenetics
Abbr.
Immunogenetics
ISSN
0093-7711
DOI
10.1007/BF00346019
Published
1981-00-00
Pages
381-91
Language
English
Region
United States
NLM ID
0420404
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00346019 View journal details
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