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PMID: 6961458 Published · ppublish English Journal Article

12-O-tetradecanoylphorbol 13-acetate-inducible proteins are synthesized at an increased rate in Bloom syndrome fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 24 ·1982-12-00 ·Pages 7886-90

Mallick U, Rahmsdorf HJ, Yamamoto N, Ponta H, Wegner RD, Herrlich P

Abstract

A set of proteins, which in normal fibroblasts were barely, if at all, detectable, were synthesized at an increased rate in fibroblasts from patients with Bloom syndrome (BS). The same set of proteins was induced in normal human fibroblasts by treatment with 12-O-tetradecanoylphorbol 13-acetate (TPA). In BS cells, TPA caused a further 2-fold increase in the rate of synthesis. Production of these proteins was inhibited by the addition of fluocinolone acetonide to the culture medium. One of the proteins (XHF1) present at high levels in BS fibroblasts and in TPA-treated cells was also induced by irradiation with ultraviolet light. This protein was secreted into the culture medium. Most other TPA-inducible proteins were cytoplasmic. Among other human mutants prone to chromosome aberrations we found one of three tested cases of Fanconi anemia and one case of ataxia-telangiectasia that showed increased spontaneous rates of synthesis of the TPA-inducible proteins. In these cases, however, the induction by TPA was like that seen in healthy fibroblasts.

MeSH Terms
Bloom Syndrome/genetics Cells, Cultured Cytoplasm/metabolism Humans Isoelectric Point Molecular Weight Phorbols/pharmacology Proteins/genetics Sister Chromatid Exchange Tetradecanoylphorbol Acetate/pharmacology Ultraviolet Rays
Chemicals
Phorbols Proteins Tetradecanoylphorbol Acetate
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Mallick U
Rahmsdorf H J
Yamamoto N
Ponta H
Wegner R D
Herrlich P
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.24.7886
Published
1982-12-00
Pages
7886-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC347454
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.79.24.7886 PMC Full Text View journal details
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