Home LiteratureArticle Details
PMID: 6956858 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Partial purification and characterization of the mRNA for human thymidine kinase and hypoxanthine/guanine phosphoribosyltransferase.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 14 ·1982-07-00 ·Pages 4290-4

Lin PF, Yamaizumi M, Murphy PD, Egg A, Ruddle FH

Abstract

We used direct microinjection of poly(A)+RNA into individual hypoxanthine/guanine phosphoribosyltransferase-deficient or thymidine kinase-deficient cells and detected the specific in vivo translation products as an assay for human hypoxanthine/guanine phosphoribosyltransferase or thymidine kinase mRNAs. The incorporation of [3H]hypoxanthine or [3H]thymidine into cells in response to injected mRNA was assayed in situ by autoradiography. Methylmercuric hydroxide/agarose gel analysis showed that human hypoxanthine/guanine phosphoribosyltransferase mRNA contains approximately 1,530 nucleotides, which is twice the number required for its protein coding capacity. The mRNA for human cytoplasmic thymidine kinase is estimated to be approximately the same length; thus, the size of the cytosol thymidine kinase subunit can be predicted to be approximately 47,000 daltons, if the full coding capacity of its mRNA is utilized.

MeSH Terms
Biological Assay Humans Hypoxanthine Phosphoribosyltransferase/genetics Molecular Weight Protein Biosynthesis RNA, Messenger/isolation & purification Thymidine Kinase/genetics
Chemicals
RNA, Messenger Hypoxanthine Phosphoribosyltransferase Thymidine Kinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Lin P F
Yamaizumi M
Murphy P D
Egg A
Ruddle F H
References (42)
42 references, click to expand
  1. Possible peptide chain termination mutants in thymide kinase gene of a mammalian virus, herpes simplex virus.
    Proc Natl Acad Sci U S A. 1975 Oct;72(10):4081-4 PMID: 172894
  2. "Early" simian-virus-40-specific RNA contains information for tumor antigen formation and chromatin replication.
    Proc Natl Acad Sci U S A. 1976 Feb;73(2):366-70 PMID: 174105
  3. Molecular-weight determination of animal-cell RNA by electrophoresis in formamide under fully denaturing conditions on exponential polyacrylamide gels.
    Eur J Biochem. 1976 Feb 16;62(2):313-22 PMID: 1253794
  4. Methylmercury as a reversible denaturing agent for agarose gel electrophoresis.
    Anal Biochem. 1976 Jan;70(1):75-85 PMID: 1259158
  5. Human deoxythymidine kinase. I. Purification and general properties of the cytoplasmic and mitochondrial isozymes derived from blast cells of acute myelocytic leukemia.
    J Biol Chem. 1976 May 10;251(9):2600-4 PMID: 1063125
  6. Thymidine kinase, DNA synthesis and cancer.
    Mol Cell Biochem. 1976 Jun 15;11(3):161-82 PMID: 940549
  7. Molecular analysis of the histone gene cluster of Psammechinus miliaris: I. Fractionation and identification of five individual histone mRNAs.
    Cell. 1976 Aug;8(4):455-69 PMID: 954099
  8. Microinjection studies of duck globin messenger RNA translation in human and avian cells.
    Cell. 1976 Dec;9(4 PT 2):725-32 PMID: 1035136
  9. Messenger RNA in heat-shocked Drosophila cells.
    J Mol Biol. 1977 Feb 5;109(4):559-87 PMID: 403287
  10. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  11. Detection of herpes simplex virus thymidine kinase polypeptides in cells labeled with 35S-methionine.
    Intervirology. 1978;9(3):162-72 PMID: 201586
  12. Thymidine kinase activity in synchronized HeLa cell cultures.
    Biochem Biophys Res Commun. 1965 Aug 16;20(4):535-8 PMID: 5860172
  13. Variations in phosphokinase activities during the cell cycle in synchronous populations of HeLa cells.
    Nature. 1965 Jul 10;207(993):176-7 PMID: 5886110
  14. Enzyme defect associated with a sex-linked human neurological disorder and excessive purine synthesis.
    Science. 1967 Mar 31;155(3770):1682-4 PMID: 6020292
  15. Mammalian cell fusion: studies on the regulation of DNA synthesis and mitosis.
    Nature. 1970 Jan 10;225(5228):159-64 PMID: 5409962
  16. Assignment of nucleoside phosphorylase to D-14 and localization of X-linked loci in man by somatic cell genetics.
    Nat New Biol. 1973 Feb 7;241(110):180-2 PMID: 4512579
  17. Properties of mitochondrial thymidine kinases of parental and enzyme-deficient HeLa cells.
    Arch Biochem Biophys. 1973 Oct;158(2):503-13 PMID: 4361106
  18. Gel electrophoresis and isoelectric focusing of mitochondrial and viral-induced thymidine kinases.
    Int J Cancer. 1974 Feb 15;13(2):203-18 PMID: 4817574
  19. Herpes simplex virus-specific polypeptides studied by polyacrylamide gel electrophoresis of immune precipitates.
    J Gen Virol. 1974 Feb;22(2):171-85 PMID: 4362401
  20. Subunit molecular weight of human hypoxanthine-guanine phosphoribosyltransferase.
    J Biol Chem. 1974 Jul 10;249(13):4038-40 PMID: 4854516
  21. Assignment of the gene for galactokinase to human chromosome 17 and its regional localisation to band q21-22.
    Nature. 1974 Oct 18;251(5476):633-6 PMID: 4371022
  22. Expression of thymidine kinase variants is a function of the replicative state of cells.
    Cell. 1974 Jun;2(2):113-7 PMID: 4455378
  23. Units of transcription and translation: sequence components of heterogeneous nuclear RNA and messenger RNA.
    Cell. 1975 Feb;4(2):77-93 PMID: 1125979
  24. Human mitochondrial thymidine kinase is coded for by a gene on chromosome 16 of the nucleus.
    Somatic Cell Genet. 1977 May;3(3):237-45 PMID: 605384
  25. Human hypoxanthine-guanine phosphoribosyltransferase. Evidence for tetrameric structure.
    J Biol Chem. 1978 Jun 25;253(12):4459-63 PMID: 659426
  26. Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme.
    Nucleic Acids Res. 1978 Jul;5(7):2333-44 PMID: 209407
  27. Human and mouse hypoxanthine-guanine phosphoribosyltransferase: dimers and tetramers.
    Science. 1979 Jan 12;203(4376):174-6 PMID: 569362
  28. The HPRT locus.
    Cell. 1979 Jan;16(1):1-9 PMID: 369702
  29. Cloning of the active thymidine kinase gene of herpes simplex virus type 1 in Escherichia coli K-12.
    Proc Natl Acad Sci U S A. 1979 Aug;76(8):3755-9 PMID: 226978
  30. Biological detection of specific mRNA molecules by microinjection.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4503-6 PMID: 291982
  31. Microinjection of early SV40 DNA fragments and T antigen.
    Methods Enzymol. 1980;65(1):816-25 PMID: 6246377
  32. Isolation of the chicken thymidine kinase gene by plasmid rescue.
    Nature. 1980 May 22;285(5762):207-10 PMID: 6246445
  33. Heterogeneity of poly(I) x poly(C)-induced human fibroblast interferon mRNA species.
    Nature. 1980 Nov 6;288(5786):95-7 PMID: 6159547
  34. Human liver thymidine kinase. Purification and some properties of the enzyme.
    J Biol Chem. 1980 Dec 10;255(23):11290-5 PMID: 7192285
  35. Biochemical transformation of thymidine kinase (TK)-deficient mouse cells by herpes simplex virus type 1 DNA fragments purified from hybrid plasmids.
    Nucleic Acids Res. 1980 Nov 25;8(22):5233-53 PMID: 6258139
  36. The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.
    Nucleic Acids Res. 1980 Dec 20;8(24):5949-64 PMID: 6258156
  37. Nucleotide sequence of the thymidine kinase gene of herpes simplex virus type 1.
    Proc Natl Acad Sci U S A. 1981 Mar;78(3):1441-5 PMID: 6262799
  38. Photoengraving of coverslips and slides to facilitate monitoring of micromanipulated cells or chromosome spreads.
    Exp Cell Res. 1981 Aug;134(2):485-8 PMID: 7023960
  39. Cloned cDNA sequences of the hypoxanthine/guanine phosphoribosyltransferase gene from a mouse neuroblastoma cell line found to have amplified genomic sequences.
    Proc Natl Acad Sci U S A. 1982 Mar;79(6):1950-4 PMID: 6952245
  40. Chemical suppression of the immune response.
    Pharmacol Rev. 1963 Jun;15:365-405 PMID: 13954804
  41. DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. I. PURIFICATION AND SOME PROPERTIES OF THE ENZYME.
    J Biol Chem. 1964 Jan;239:269-74 PMID: 14114853
  42. DEOXYTHYMIDINE KINASE OF ESCHERICHIA COLI. II. KINETICS AND FEEDBACK CONTROL.
    J Biol Chem. 1964 Jan;239:275-84 PMID: 14114854
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.14.4290
Published
1982-07-00
Pages
4290-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346656
Subset
IM
Grants
NIA NIH HHS · 5 R01 AGD1940-02 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.14.4290 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2