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PMID: 6953428 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

beta-globin transcript found in induced murine erythroleukemia cells is homologous to the beta h0 and beta h1 genes.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 79 ·No. 9 ·1982-05-00 ·Pages 2753-7

Brown BA, Padgett RW, Hardies SC, Hutchison CA, Edgell MH

Abstract

RNA transcripts complementary to at least one of the four beta-globin homologous genes (beta h) are found in high concentration in the murine erythroleukemic (MEL) cell line GM979 after butyric acid induction. Hybridization data indicate that the gene expressed is Hbb-beta h0 or Hbb-beta h1, or both. The level of beta h0/1 transcripts in the MEL cell is similar to the level of adult transcripts. The Hbb-beta h0/1 transcript is about 800 nucleotides in length. In addition, there is a larger beta h0/1 transcript of the same size and relative intensity as the adult beta-globin precursor. We also report significant levels of embryonic gene Hbb-y transcripts in induced GM979 cells. We have determined that the GM979 cell line has the [Hbb]s haplotype on the basis of an examination of its globin DNA restriction pattern. An additional MEL cell line of haplotype [Hbb]d (DBA/2 line 6A11A) was examined and found to contain no significant level of Hbb-beta h0, Hbb-beta h1, Hbb-beta h2, or Hbb-y gene transcripts.

MeSH Terms
Animals Base Sequence Cell Line Genes Globins/genetics Leukemia, Erythroblastic, Acute/genetics Mice Nucleic Acid Hybridization RNA, Messenger/genetics Transcription, Genetic
Chemicals
RNA, Messenger Globins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Brown B A
Padgett R W
Hardies S C
Hutchison C A
Edgell M H
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38 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.79.9.2753
Published
1982-05-00
Pages
2753-7
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC346284
Subset
IM
Grants
NIAID NIH HHS · AI08998 · United States
NIGMS NIH HHS · GM21313 · United States
External Links
PubMed source DOI: 10.1073/pnas.79.9.2753 PMC Full Text View journal details
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