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PMID: 6893198 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Shape and volume changes in erythrocyte ghosts and spectrin-actin networks.

The Journal of cell biology ·Vol. 86 ·No. 2 ·1980-08-00 ·Pages 371-6

Johnson RM, Taylor G, Meyer DB

Abstract

In response to changes in electrolyte concentration and pH, erythrocyte ghosts can exhibit some of the characteristic shapes seen in the intact erythrocyte. These shape changes are accompanied by volume changes; both are reversible, not energy dependent, and not inhibited by sulfhydryl reagents. The volume reduction can also be seen in isolated Triton-free spectrin-actin lattices, showing that this network is capable of reversible contraction. The results suggest that reversible changes in size of the underlying cytoskeleton of the erythrocyte membrane can control cell shape.

MeSH Terms
Actins/blood,metabolism Adenosine Triphosphate/pharmacology Cytoskeleton/ultrastructure Erythrocyte Membrane/ultrastructure Erythrocytes/ultrastructure Humans Hydrogen-Ion Concentration Membrane Proteins/metabolism Polyethylene Glycols/pharmacology Protein Binding Salts/pharmacology Sodium Chloride/pharmacology Spectrin/metabolism
Chemicals
Actins Membrane Proteins Salts Spectrin Polyethylene Glycols Sodium Chloride Adenosine Triphosphate
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Johnson R M
Taylor G
Meyer D B
References (26)
26 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.86.2.371
Published
1980-08-00
Pages
371-6
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2111495
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.86.2.371 PMC Full Text View journal details
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