Home LiteratureArticle Details
PMID: 490624 Published · ppublish English Journal Article

Hemoglobin-depleted human erythrocyte ghosts: characterization of morphology and transport functions.

The Journal of membrane biology ·Vol. 48 ·No. 1 ·1979-06-29 ·Pages 43-67

Bjerrum PJ

Abstract

A method of preparing hemoglobin-depleted resealed ghosts with an extremely low hemoglobin content is described. The membrane morphology, the crossed immunoelectrophoresis pattern of the membrane proteins, and the transport function of these ghosts have been examined. Electron microscopic examination of the ghosts on hydrophilic as well as hydrophobic grid surfaces revealed a faint filamentous network (spectrin) associated with the membrane. The ghosts were found to have permeabilities towards small polar molecules (water and mannitol) and ions (chloride, sodium, and potassium) which are quantitatively very close to those of intact red cells. It is concluded that white ghosts prepared by the present method are well suited for simultaneous studies of morphology, membrane biochemistry, and membrane transport functions.

MeSH Terms
Biological Transport, Active Cell Fractionation/methods Erythrocyte Membrane/metabolism,ultrastructure Erythrocytes/metabolism Hemoglobins/metabolism Humans Immunoelectrophoresis Membrane Proteins/metabolism Microscopy, Electron, Scanning
Chemicals
Hemoglobins Membrane Proteins
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Bjerrum P J
References (34)
34 references, click to expand
  1. Factors controlling the resealing of the membrane of human erythrocyte ghosts after hypotonic hemolysis.
    J Membr Biol. 1972;8(1):1-26 PMID: 4628383
  2. Chemical modification of membrane proteins in relation to inhibition of anion exchange in human red blood cells.
    J Cell Physiol. 1975 Dec;86(3 Pt 1):471-94 PMID: 1202029
  3. Solubilization of membranes by detergents.
    Biochim Biophys Acta. 1975 Mar 25;415(1):29-79 PMID: 1091302
  4. Is hemoglobin an essential structural component of human erythrocyte membranes?
    J Clin Invest. 1963 Apr;42:581-8 PMID: 13999462
  5. Determination of sodium, potassium, and water in human red blood cells. Elimination of sources of error in the development of a flame photometric method.
    Scand J Clin Lab Invest. 1966;18(2):151-66 PMID: 5914741
  6. The sub-membrane reticulum of the human erythrocyte: a scanning electron microscope study.
    J Supramol Struct. 1977;6(3):301-11 PMID: 592817
  7. A manual of quantitative immunoelectrophoresis. Methods and applications. 1. General remarks on principles, equipment, reagents and procedures.
    Scand J Immunol Suppl. 1973;1:15-35 PMID: 4129691
  8. The preparation and chemical characteristics of hemoglobin-free ghosts of human erythrocytes.
    Arch Biochem Biophys. 1963 Jan;100:119-30 PMID: 14028302
  9. Evidence of high stability of the glucose transport carrier function in human red cell ghosts extensively washed in various media.
    Arch Biochem Biophys. 1971 Sep;146(1):215-26 PMID: 5004123
  10. Characteristics of chloride transport in human red blood cells.
    J Gen Physiol. 1973 Feb;61(2):185-206 PMID: 4688320
  11. The function of calcium in the potassium permeability of human erythrocytes.
    Biochim Biophys Acta. 1958 Dec;30(3):653-4 PMID: 13618284
  12. A direct method for the quantitative measurement of red cell dimensions.
    J Lab Clin Med. 1961 May;57:819-24 PMID: 13784610
  13. The kinetics of resealing of washed erythrocyte ghosts.
    J Membr Biol. 1975 Jul 24;22(3-4):231-53 PMID: 808630
  14. The immunochemical approach to the characterization of membrane proteins. Human erythrocyte membrane proteins analysed as a model system.
    Biochim Biophys Acta. 1976 Nov 11;455(1):66-89 PMID: 990330
  15. A multipurpose specimen-carrier for handling small biological objects through critical point drying.
    J Microsc. 1975 Sep;105(1):107-13 PMID: 813001
  16. Chloride transport in human erythrocytes and ghosts: a quantitative comparison.
    J Physiol. 1976 Nov;262(3):679-98 PMID: 13204
  17. Effects of bicarbonate on lithium transport in human red cells.
    J Gen Physiol. 1978 Jun;71(6):721-46 PMID: 670928
  18. Size distribution of erythrocytes.
    Ann N Y Acad Sci. 1962 Jun 29;99:242-61 PMID: 13872710
  19. Crossed immunoelectrophoresis of human erythrocyte membrane proteins. Immunoprecipitation patterns for fresh and stored samples of membranes extensively solubilized with non-ionic detergents.
    Biochim Biophys Acta. 1974 Mar 14;342(1):69-80 PMID: 4856866
  20. Temperature-dependent changes of chloride transport kinetics in human red cells.
    J Gen Physiol. 1977 Sep;70(3):283-306 PMID: 19556
  21. Water-soluble proteins of the human red cell membrane.
    J Membr Biol. 1970 Dec;3(1):156-72 PMID: 24174191
  22. Preparation and properties of human erythrocyte ghosts.
    Mol Cell Biochem. 1973 Dec 15;2(2):197-218 PMID: 4272551
  23. Temperature dependence of chloride, bromide, iodide, thiocyanate and salicylate transport in human red cells.
    J Physiol. 1972 Aug;224(3):583-610 PMID: 5071931
  24. Water permeability of thin lipid membranes.
    J Gen Physiol. 1967 Jul;50(6):1765-84 PMID: 6034767
  25. Polyol permeability of the human red cell. Interpretation of glucose transport in terms of a pore.
    Biochim Biophys Acta. 1977 Apr 1;466(1):68-83 PMID: 856270
  26. Some effects of low pH on chloride exchange in human red blood cells.
    J Gen Physiol. 1975 Jun;65(6):731-49 PMID: 459
  27. On the ultrastructure of the plasma membrane as determined by the electron microscope.
    J Cell Comp Physiol. 1953 Oct;42(2):203-47 PMID: 13096528
  28. Synthesis of tritiated 4,4'-diisothiocyano-2,2'-stilbene disulfonic acid ([3H]DIDS) and its covalent reaction with sites related to anion transport in human red blood cells.
    J Membr Biol. 1977 May 12;33(3-4):311-23 PMID: 864693
  29. A study of the relationship between inhibition of anion exchange and binding to the red blood cell membrane of 4,4'-diisothiocyano stilbene-2,2'-disulfonic acid (DIDS) and its dihydro derivative (H2DIDS).
    J Membr Biol. 1976 Oct 20;29(1-2):147-77 PMID: 978716
  30. Properties of water channels in human red cells.
    Biomembranes. 1972;3:331-40 PMID: 4666514
  31. Effect of ouabain on gluclose metabolism and on fluxes of sodium and potassium of human blood cells.
    Acta Physiol Scand. 1967 Sep;71(1):113-24 PMID: 6056954
  32. The organization of proteins in the human red blood cell membrane. A review.
    J Cell Biol. 1974 Jul;62(1):1-19 PMID: 4600883
  33. Membrane proteins related to anion permeability of human red blood cells. I. Localization of disulfonic stilbene binding sites in proteins involved in permeation.
    J Membr Biol. 1974;15(3):207-26 PMID: 4838037
  34. Polypeptide analysis of individual immunoprecipitates from crossed immunoelectrophoresis.
    Anal Biochem. 1977 Aug;81(2):432-41 PMID: 562077
Article Info
Journal
The Journal of membrane biology
Abbr.
J Membr Biol
ISSN
0022-2631
DOI
10.1007/BF01869256
Published
1979-06-29
Pages
43-67
Language
English
Region
United States
NLM ID
0211301
Subset
IM
External Links
PubMed source DOI: 10.1007/BF01869256 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2